Results 51 to 60 of about 80,578 (289)

Crucial parameters for precise copy number variation detection in formalin‐fixed paraffin‐embedded solid cancer samples

Molecular Oncology, EarlyView.
This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris, Vasiliki Siozopoulou, Léon C van Kempen, Anne Sieben, Ella Roelant, Stig Hellemans, Elyne Backx, Laure Sorber, Koen De Winne, Senada Koljenović, Karen Zwaenepoel   +10 more
wiley   +1 more source

Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer

Molecular Oncology, EarlyView.
PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta, Yang Song, Madeleine Ndahayo, Anirudh Saxena, Theresa Guo, Dylan Z. Kelley, Jessica Gore, Andrew Hennigan, Alexa Anderson, John C. Papadimitriou, Daria A. Gaykalova   +10 more
wiley   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Microarray-based optimization to detect genomic deletion mutations

Genomics Data, 2014
We performed array comparative genome hybridization (aCGH) analyses of five Arabidopsis thaliana mutants with genomic deletions ranging in size from 4 bp to >5 kb.
Eric J. Belfield, Carly Brown, Xiangchao Gan, Caifu Jiang, Dilair Baban, Aziz Mithani, Richard Mott, Jiannis Ragoussis, Nicholas P. Harberd   +8 more
doaj   +1 more source

Impact of a senior research thesis on students' perceptions of scientific inquiry in distinct student populations

FEBS Open Bio, EarlyView.
This study addressed how a senior research thesis is perceived by undergraduate students. It assessed students' perception of research skills, epistemological beliefs, and career goals in Biochemistry (science) and BDC (science‐business) students. Completing a thesis improved confidence in research skills, resilience, scientific identity, closed gender‐
Celeste Suart, Haley L. Zubyk, Michelle Ogrodnik, Caitlin E. Mullarkey, Felicia Vulcu   +4 more
wiley   +1 more source

Genomic alterations detected by comparative genomic hybridization in ovarian endometriomas

Brazilian Journal of Medical and Biological Research, 2010
Endometriosis is a complex and multifactorial disease. Chromosomal imbalance screening in endometriotic tissue can be used to detect hot-spot regions in the search for a possible genetic marker for endometriosis. The objective of the present study was to
L.C. Veiga-Castelli, J.C. Rosa e Silva, J. Meola, R.A. Ferriani, M. Yoshimoto, S.A. Santos, J.A. Squire, L. Martelli   +7 more
doaj  

First Trials of Genome Analyses in Some Onobrychis Species using Dot-Blot and Genomic in situ Hybridization Techniques

Journal of Advanced Research in Natural and Applied Sciences
The origin and genome composition of tetraploid cultivated Onobrychis viciifolia (2n = 4x = 28) were analyzed using dot-blot and genomic in situ hybridization (GISH) techniques.
Gülru Yücel, Bozena Kolano, Evren Cabi, Metin Tuna   +3 more
doaj   +1 more source

arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays

BMC Bioinformatics, 2005
Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered and accelerated the use of several platforms for analysis of DNA ...
Moreau Yves, Vermeesch Joris, van Vooren Steven, De Paepe Anne, Mortier Geert, Buysse Karen, Michels Evi, Robbrecht Piet, De Preter Katleen, Pattyn Filip, Menten Björn, De Moor Bart, Vermeulen Stefan, Speleman Frank, Vandesompele Jo   +14 more
doaj   +1 more source

Comparative genomic hybridization of postirradiation sarcomas [PDF]

Cancer, 2001
Radiotherapy is a known risk factor for sarcoma development. Postirradiation sarcomas arise within the radiation field after a latency period of several years and usually are highly malignant. Very little is yet known about their genetic changes.Twenty-seven postirradiation sarcomas were analyzed by comparative genomic hybridization, which allows ...
M, Tarkkanen, T A, Wiklund, M J, Virolainen, M L, Larramendy, N, Mandahl, F, Mertens, C P, Blomqvist, E J, Tukiainen, M M, Miettinen, A I, Elomaa, Y S, Knuutila   +10 more
openaire   +2 more sources

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source