Results 171 to 180 of about 1,688,140 (295)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Mechanistic insights into complement inhibition

Doctor of PhilosophyBiochemistry and Molecular Biophysics Interdepartmental ProgramBrian V. GeisbrechtThe complement system is a fundamental component of innate immunity, acting as a first line of defense against pathogens, maintaining immune homeostasis,
Duan, Huiquan
core  

Complement Inhibition Therapy in Myasthenic Crisis-A Multicentre Retrospective Analysis of 17 Cases From Germany. [PDF]

Eur J Neurol
Gerischer L, Stein M, Schneider A, Tangen H, Loris J, Glaubitz S, Zschüntzsch J, Oy UH, Schubert C, Heesen C, Schlag M, Hagenacker T, Oeztuerk M, Ruck T, Doksani P, Dusemund C, Herdick M, Herzig-Nichtweiß J, Mergenthaler P, Stascheit F, Suboh A, Schwarz L, Hoffmann S, Meisel A, Lehnerer S.   +24 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

How Severe is Severe Enough? Organ Failure Rather Than Anemia Severity as a Potential Threshold for Complement Inhibition in Pediatric Paroxysmal Cold Hemoglobinuria: A Case Report. [PDF]

Case Rep Pediatr
Yu C, Rane Z, Nedelcu E, Matsunaga A.
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Complement Inhibition for Acute Neuromyelitis Optica Spectrum Disorder Attacks: Insights From an International Case Series. [PDF]

Neurol Neuroimmunol Neuroinflamm
Rommer PS, Jiang W, Nolte JP, Mikami T, De Seze J, Sánchez P, Harel A, Alkabie S, Kaneko K, Bilodeau PA, Misu T, Kremer L, Bigaut K, Leypoldt F, Aktas O, Ringelstein M, Siffrin V, Zhang LJ, Cong H, Lowe M, Barreras P, Chen H, Piquet AL, Sotirchos ES, Kammeyer R, Zook J, Bennett JL, Berger T, Fujihara K, Levy M, Shi FD, Paul F.   +31 more
europepmc   +1 more source

Complement Inhibition in Kidney Transplantation: Where Are We Now? [PDF]

BioDrugs, 2023
Vonbrunn E, Büttner-Herold M, Amann K, Daniel C.   +3 more
europepmc   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Case report: Sequential complement inhibition and BAFF/APRIL blockade in progressive IgA nephropathy and IgA vasculitis nephritis: a report of two cases. [PDF]

Front Immunol
Tian X, Hou W, Yu X, Jiang L, Zhang X, Zhang X, Wang S, Tan Y, Liu L.   +8 more
europepmc   +1 more source