Results 1 to 10 of about 242,920 (141)

Clinical Evaluation of Direct Reverse Transcription PCR for Detection of SARS-CoV-2 Compared to Conventional RT-PCR in Patients with Positive Rapid Antigen Test Results during Circulation of Emerging Viral Variants [PDF]

Diagnostics, 2023
The emergence of the Omicron (B.1.1.529) variant of SARS-CoV-2 has precipitated a new global wave of the COVID-19 pandemic. The rapid identification of SARS-CoV-2 infection is imperative for the effective mitigation of transmission. Diagnostic modalities
Ming-Jr Jian, Chi-Sheng Chen, Hsing-Yi Chung, Chih-Kai Chang, Cherng-Lih Perng, Hung-Sheng Shang   +5 more
doaj   +2 more sources

A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories. [PDF]

PLoS ONE, 2013
Rabies is a lethal and notifiable zoonotic disease for which diagnostics have to meet the highest standards. In recent years, an evolution was especially seen in molecular diagnostics with a wide variety of different detection methods published ...
Melina Fischer, Kerstin Wernike, Conrad M Freuling, Thomas Müller, Orhan Aylan, Bernard Brochier, Florence Cliquet, Sonia Vázquez-Morón, Peter Hostnik, Anita Huovilainen, Mats Isaksson, Engbert A Kooi, Jean Mooney, Mihai Turcitu, Thomas B Rasmussen, Sandra Revilla-Fernández, Marcin Smreczak, Anthony R Fooks, Denise A Marston, Martin Beer, Bernd Hoffmann   +20 more
doaj   +21 more sources

Improved Detection Sensitivity of Spring Viremia of Carp Virus by Substituting a Two-Step with a One-Step Nested Reverse Transcription Polymerase Chain Reaction Method [PDF]

Microorganisms
Spring viremia of carp (SVC) is a highly contagious disease that affects cyprinids, resulting in systemic hemorrhage, abdominal distension, exophthalmia, and high mortality in juveniles.
Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon   +6 more
doaj   +2 more sources

Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]

Veterinary World, 2021
Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid, Eman I. M. Beleta, Mohamed A. Kelany, Rania I. Ismail, Nadia A. Shalaby, Manal H. M. Khafagi   +5 more
doaj   +1 more source

Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato

Pathogens, 2022
In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature
Marta Luigi, Ariana Manglli, Antonio Tiberini, Sabrina Bertin, Luca Ferretti, Anna Taglienti, Francesco Faggioli, Laura Tomassoli   +7 more
doaj   +1 more source

A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types

Journal of Integrative Agriculture, 2020
To develop a rapid and high-sensitivity method for detection of grapevine virus E (GVE), a SYBR Green based real-time fluorescence quantitative RT-PCR method (RT-qPCR) was established.
Fang REN, Zun-ping ZHANG, Xu-dong FAN, Guo-jun HU, Meng-yan ZHANG, Ya-feng DONG   +5 more
doaj   +1 more source

Performance of Simplexa dengue molecular assay compared to conventional and SYBR green RT-PCR for detection of dengue infection in Indonesia. [PDF]

PLoS ONE, 2014
Diagnostic tests based on detection of dengue virus (DENV) genome are available with varying sensitivities and specificities. The Simplexa Dengue assay (Focus Diagnostics) is a newly developed real-time RT-PCR method designed to detect and serotype DENV ...
R Tedjo Sasmono, Aryati Aryati, Puspa Wardhani, Benediktus Yohan, Hidayat Trimarsanto, Sukmal Fahri, Tri Y Setianingsih, Febrina Meutiawati   +7 more
doaj   +1 more source

Comparison of 16S rRNA Gene Quantification with Selected Inflammatory Markers and Culture Method in Septic Arthritis

Journal of Pure and Applied Microbiology, 2023
This study aims to determine the types of causative organism, the utility of synovial procalcitonin (PCT), C-Reactive Protein (CRP) and bacterial 16S rRNA gene-based RT-PCR and their comparison with conventional culture results in patients with ...
Rana Abdulrahman Hamood Al-Masoodi, Uday Younis Hussein Abdullah, Tengku Muzaffar Bin Tengku Mohd Shihabudin, Ahmad Fadzli Bin Solong, Salwani Binti Ismail, Azian Harun   +5 more
doaj   +1 more source

Establishment of reverse transcription recombinase–aided amplification-lateral-flow dipstick and real-time fluorescence–based reverse transcription recombinase–aided amplification methods for detection of the Newcastle disease virus in chickens

Poultry Science, 2020
Newcastle disease is an acute and highly contagious disease of poultry caused by Newcastle disease virus infection, which does great harm to the poultry industry all over the world.
Wenjing Wang, Chunguang Wang, Yun Bai, Peng Zhang, Shanshan Yao, Jingru Liu, Tie Zhang   +6 more
doaj   +1 more source

A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test

Scientific Reports, 2021
Shortages of reverse transcriptase (RT)-polymerase chain reaction (PCR) reagents and related equipment during the COVID-19 pandemic have demonstrated the need for alternative, high-throughput methods for severe acute respiratory syndrome coronavirus-2 ...
Jonas Schmidt, Sandro Berghaus, Frithjof Blessing, Folker Wenzel, Holger Herbeck, Josef Blessing, Peter Schierack, Stefan Rödiger, Dirk Roggenbuck   +8 more
doaj   +1 more source