Results 11 to 20 of about 243,069 (278)

Noroviruses Surrogate Detection Using Loop-Mediated Isothermal Amplification (LAMP), Conventional RT-PCR and Quantitative RT-PCR in Sg. Tekala and Sg. Gabai Stream Water [PDF]

MATEC Web of Conferences, 2017
A preliminary study was carried out in order to evaluate a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detecting Noroviruses (NoV) RNA surrogates as external standard for NoV.The detection limit of the NoV RT-LAMP ...
Khairuddin Dzulaikha, Mohd Yusof Nurul Yuziana, Jani Jan Maizatulriah, Ab. Wahid Marfiah   +3 more
doaj   +2 more sources

Gel express: a novel frugal method quantifies gene relative expression in conventional RT-PCR [PDF]

Beni-Suef University Journal of Basic and Applied Sciences, 2022
Background Real-time PCR system is a valuable scientific mainstream needed for quantifying specific gene expression. Nevertheless, compared with conventional PCR, the real-time PCR system is extremely expensive and not affordable for limited or mid ...
Mohamed Hazman
doaj   +2 more sources

Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR [PDF]

Virology Journal, 2011
Background Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on
Lou Sai, Li Zhu, Zhang Guoyu, Chen Jinghong, Qiu Jianming, Han Qunying, Liu Zhengwen, Zhang Ni, Li Na   +8 more
doaj   +4 more sources

Human rhinovirus screening conventional RT-PCR ("Gama assay") v2 [PDF]

, 2018
This is a very handy, previously published [Ref 1 and 2], rhinovirus (RV) screening assay which I and many others have used on many sample extracts, mostly originating fromacutely ill paediatric patients, spanning well over a decade's worth of collection dates.
Ian M Mackay, Katherine E. Arden
  +7 more sources

Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A Comparação entre as técnicas de RT-PCR convencional e RT-PCR em tempo real para a detecção do metapneumovírus aviários subtipo A

Ciência Rural, 2009
Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV.
Helena Lage Ferreira, Fernando Rosado Spilki, Márcia Mercês Aparecida Bianchi dos Santos, Renata Servan de Almeida, Clarice Weis Arns   +4 more
doaj   +1 more source

Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR. [PDF]

Braz J Microbiol, 2020
In March 2020, WHO declared a pandemic state due to SARS-CoV-2 having spread. TaqMan-based real-time RT-qPCR is currently the gold standard for COVID-19 diagnosis. However, it is a high-cost assay, inaccessible for the majority of laboratories around the world, making it difficult to diagnose on a large scale.
Dorlass EG, Monteiro CO, Viana AO, Soares CP, Machado RRG, Thomazelli LM, Araujo DB, Leal FB, Candido ED, Telezynski BL, Valério CA, Chalup VN, Mello R, Almeida FJ, Aguiar AS, Barrientos ACM, Sucupira C, De Paulis M, Sáfadi MAP, Silva DGBP, Sodré JJM, Soledade MP, Matos SF, Ferreira SR, Pinez CMN, Buonafine CP, Pieroni LNF, Malta FM, Santana RAF, Souza EC, Fock RA, Pinho JRR, Ferreira LCS, Botosso VF, Durigon EL, Oliveira DBL.   +35 more
europepmc   +4 more sources

Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay. [PDF]

Biomed Res Int, 2018
Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection.
Ma L, Zeng F, Huang B, Cong F, Huang R, Ma J, Guo P.   +6 more
europepmc   +4 more sources

DETECTION SENSITIVITY AND QUANTITATION OF PLASMODIUM FALCIPARUM VAR GENE TRANSCRIPTS BY REAL-TIME RT-PCR IN COMPARISON WITH CONVENTIONAL RT-PCR [PDF]

The American Journal of Tropical Medicine and Hygiene, 2006
Antigenic variation in Plasmodium falciparum erythrocyte membrane protein 1, caused by a switch in transcription of the encoding var gene, is an important feature of malaria. In this study, we quantified the relative abundance of var gene transcripts present in P.
Gatton, Michelle L., Peters, Jennifer M., Gresty, Karryn, Fowler, Elizabeth V., Chen, Nanhua, Cheng, Qin   +5 more
openaire   +2 more sources

Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A [PDF]

Ciência Rural, 2009
Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR ...
Ferreira, Helena Lage, Spilki, Fernando Rosado, Santos, Márcia Mercês Aparecida Bianchi dos, Almeida, Renata Servan de, Arns, Clarice Weis   +4 more
openaire   +3 more sources

Optimization of RT-PCR methods for enterovirus detection in groundwater

Heliyon, 2023
Enteroviruses (EVs), which belong to the Picornaviridae family, infect individuals asymptomatically or cause mild symptoms (fever, runny nose, cough, skin rash, sneezing, mouth blister).
Boeun Yoo, Mi-Gyeong Kim, A Young Min, Doo Won Seo, Seung Hwan Kim, Soon Han Kim   +5 more
doaj   +1 more source