Results 21 to 30 of about 243,069 (278)

Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay [PDF]

The Plant Pathology Journal, 2022
Cymbidium mosaic virus (CymMV) is one of economically important viruses that cause significant losses of orchids in the world. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow ...
Do-Hyun Kim, Rae-Dong Jeong, Sena Choi, Ho-Jong Ju, Ju-Yeon Yoon   +4 more
doaj   +1 more source

Performance of Chip Based Real Time RT- PCR (TrueNat) and Conventional Real Time RT-PCR for Detection of SARS-CoV-2

JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2021
Introduction: Coronavirus Disease-2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is ravaging the globe due to its rapid spread. Since providing fast results is of critical importance in a time of shortage of medical personnel and beds in isolation wards and to ensure timely treatment for patients, developing
AMIYABALA SAHOO, ANURADHA SHULANIA, MALA CHHABRA, STUTI KANSRA, ARVIND ACHRA, KIRTI NIRMAL, SUPRIYA KATIYAR, NANDINI DUGGAL   +7 more
openaire   +2 more sources

Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR [PDF]

Journal of Virological Methods, 2016
Graft-indexing of an advanced selection from the University of Florida strawberry breeding program produced virus-like symptoms on Fragaria vesca. However; RT-PCR testing of the material did not detect the presence of any of 16 strawberry virus species or members of virus groups for which strawberries are routinely indexed.
Thanuja Thekke Veetil, Thien Ho, Catalina Moyer, Vance M. Whitaker, Ioannis E. Tzanetakis   +4 more
openaire   +2 more sources

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR [PDF]

Journal of Visualized Experiments, 2018
The aim of this method is to facilitate the fast, sensitive and specific detection of Tilapia Lake Virus (TiLV) in tilapia tissues. This protocol can be used as part of surveillance programs, biosecurity measures and in TiLV basic research laboratories.
Pamela, Nicholson, Pattarasuda, Rawiwan, Win, Surachetpong   +2 more
openaire   +2 more sources

Noninvasive prenatal testing of beta-thalassemia for common Pakistani mutations: a comparative study using cell-free fetal DNA from maternal plasma and chorionic villus sampling

Hematology, 2022
Background The discovery of circulating cell-free fetal DNA (cff-DNA) in maternal plasma has inspired the noninvasive prenatal testing (NIPT) approaches for various genetic fetal screening including rhesus D typing, sex determination, aneuploidies, and ...
Muhammad Afzal, Muhammad Abdul Naeem, Suhaib Ahmed, Nayyar Amin, Amena Rahim, Manazza Munawar, Mansoor Ishaq, Ali Rathore, K. Maria   +8 more
doaj   +1 more source

Comparison of the conventional multiplex RT–PCR, real time RT–PCR and Luminex xTAG^® RVP fast assay for the detection of respiratory viruses [PDF]

Journal of Medical Virology, 2015
Detection of respiratory viruses using polymerase chain reaction (PCR) is sensitive, specific and cost effective, having huge potential for patient management. In this study, the performance of an in‐house developed conventional multiplex RT–PCR (mRT–PCR), real time RT–PCR (rtRT–PCR) and Luminex xTAG® RVP fast assay (Luminex Diagnostics, Toronto ...
Manohar L, Choudhary, Siddharth P, Anand, Shamal A, Tikhe, Atul M, Walimbe, Varsha A, Potdar, Mandeep S, Chadha, Akhilesh C, Mishra   +6 more
openaire   +2 more sources

YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs. [PDF]

, 2017
Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles.
Honda, Shozo, Kirino, Yohei, Loher, Phillipe, Rigoutsos, Isidore, Shigematsu, Megumi, Telonis, Aristeidis G.   +5 more
core   +2 more sources

Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]

, 2005
Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T., Cloak O.M., Hani E.C., Hunt J.M., Linton D., Manser P.A., Munroe D., Ng L.-K., Penner J.L., Totten P.A., Wilson D.L.   +10 more
core   +1 more source

Quick assessment of influenza a virus infectivity with a long-range reverse-transcription quantitative polymerase chain reaction assay

BMC Infectious Diseases, 2020
Background The polymerase chain reaction (PCR) is commonly used to detect viral pathogens because of its high sensitivity and specificity. However, conventional PCR methods cannot determine virus infectivity.
Yuki Nakaya, Takashi Fukuda, Hiroki Ashiba, Masato Yasuura, Makoto Fujimaki   +4 more
doaj   +1 more source

Multi-annual performance evaluation of laboratories in post-mortem diagnosis of animal rabies: Which techniques lead to the most reliable results in practice?

PLoS Neglected Tropical Diseases, 2021
Rabies diagnosis proficiency tests on animal specimens using four techniques (FAT, RTCIT, conventional RT-PCR and real-time RT-PCR) were organised over 10 years (2009-2019). Seventy-three laboratories, of which 59% were from Europe, took part.
Emmanuelle Robardet, Alexandre Servat, Jonathan Rieder, Evelyne Picard-Meyer, Florence Cliquet   +4 more
doaj   +1 more source