Japanese Journal of Infectious Diseases, 2008 Human metapneumovirus (hMPV) is an etiologic agent of respiratory tract infections. In this study, we compared the sensitivity and specificity of real-time reverse transcription (RT)-polymerase chain reaction (PCR), conventional RT-PCR, and nested PCR in detecting hMPV genes.
Atsushi, Kaida +4 more
openaire +2 more sources
Comparison of CBNAAT and conventional real time RT PCR for HIV 1 viral load testing
Indian Journal of Medical Microbiology, 2021 HIV viral load testing is now recommended for monitoring of anti-retroviral treatment failure in PLHIV. Xpert® HIV-1 Viral Load is a fully automated CB-NAAT. A reduced turnaround time leads to prompt clinical management. Hence the current study was undertaken to compare Xpert® HIV-1 Viral Load with the routinely used conventional real time RT PCR.The ...
Nayana A, Ingole, Gita, Nataraj
openaire +2 more sources
International Journal of Veterinary Science and Medicine, 2014 This study was carried out to compare between conventional cultural isolation methods and real time polymerase chain reaction (RT-PCR) technique for the detection of Salmonella in broiler chicks.
Waleed A. Ibrahim +3 more
doaj +1 more source
A microfluidic processor for gene expression profiling of single human embryonic stem cells [PDF]
, 2008 The gene expression of human embryonic stem cells (hESC) is a critical aspect for understanding the normal and pathological development of human cells and tissues.
Chen, Yan +6 more
core +1 more source
Alternative polyadenylation of ZEB1 promotes its translation during genotoxic stress in pancreatic cancer cells [PDF]
, 2017 Pancreatic ductal adenocarcinoma (PDAC) is characterized by extremely poor prognosis. The standard chemotherapeutic drug, gemcitabine, does not offer significant improvements for PDAC management due to the rapid acquisition of drug resistance by patients.
Bielli, Pamela +7 more
core +2 more sources
Anoxic nitrification in marine sediments [PDF]
, 2004 Nitrate peaks are found in pore-water profiles in marine sediments at depths considerably below the conventional zone of oxic nitrification. These have been interpreted to represent nonsteady- state effects produced by the activity of nitrifying ...
Anschutz, P. +8 more
core +1 more source
Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology
International Journal of Medical Students, 2013 The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B. A. Saeed, Nusreen S. Ahmad
doaj +3 more sources
Timed and targeted differential regulation of nitric oxide synthase (NOS) and anti-NOS genes by reward conditioning leading to long-term memory formation [PDF]
, 2005 In a number of neuronal models of learning, signaling by the neurotransmitter nitric oxide (NO), synthesized by the enzyme neuronal NO synthase (nNOS), is essential for the formation of long-term memory (LTM). Using the molluscan model system Lymnaea, we
Benjamin, Paul R +6 more
core +1 more source
Reduced tillage, but not organic matter input, increased nematode diversity and food web stability in European long‐term field experiments [PDF]
, 2019 Soil nematode communities and food web indices can inform about the complexity, nutrient flows and decomposition pathways of soil food webs, reflecting soil quality.
De Caceres M. +22 more
core +1 more source
A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System. [PDF]
PLoS ONE, 2016 Human enteric viruses are recognized as the main causes of food- and waterborne diseases worldwide. Sensitive and quantitative detection of human enteric viruses is typically achieved through quantitative RT-PCR (RT-qPCR).
Coralie Coudray-Meunier +5 more
doaj +1 more source