Results 51 to 60 of about 2,267 (171)
mBio, 2022 The relationship between spatiotemporal distribution of HIV-1 proviruses and their transcriptional activity is not well understood. To elucidate the intranuclear positions of transcriptionally active HIV-1 proviruses, we utilized an RNA fluorescence in ...
Ryan C. Burdick +5 more
doaj +1 more source
The CPSF6-binding interface determines HIV-1 nuclear entry requirements.
, 2012 (A–B) CPSF6 residue F321 is critical for interaction with HIV-1 CAN. (A) HeLa cells expressing empty vector (white bar), HA-tagged CPSF6-358 (black bar) or CPSF6-358 bearing mutation F321N (striped bar) were infected with GFP-encoding VSV-G pseudotyped ...
Vineet N. KewalRamani (138643) +8 more
core +1 more source
Frontiers in Pharmacology, 2023 Background: 5-Fluorouracil (5-FU) is a widely used chemotherapeutic drug in clinical cancer treatment, including hepatocellular carcinoma (HCC). A correct understanding of the mechanisms leading to a low or lack of sensitivity of HCC to 5-FU-based ...
Xinyu Gu +4 more
doaj +1 more source
CPSF6 deficiency inhibits virus replication.
(A) Immunoblot analyses knocking-out efficiency of CPSF6 in A549 and L929 cells. (B) qRT–PCR analyses of VSV mRNA abundance in WT and CPSF6-/- A549 and L929 cells infected with VSV-eGFP at indicated time points.
Rong Chen (29176) +12 more
core +2 more sources
CPSF6 deficiency promotes IFN-I signalling activation.
(A, B) The heatmap shows the mRNA abundance of IFNB and ISGs in WT, Cpsf6-/-L929PLVX and Cpsf6-/- L929CPSF6 L929 cells infected with VSV-eGFP (A) or stimulated with poly (I:C) or Poly (dA:dT) (B) at indicated time points.
Rong Chen (29176) +12 more
core +2 more sources
Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus
Animal Research and One Health, EarlyView.The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary +7 more
wiley +1 more source
Tape‐Stripped Proteomics: A Comparative Analysis of Different Sample Collection Approaches
PROTEOMICS, Volume 26, Issue 11, Page 35-39, November 2026.ABSTRACT Tape‐stripping of the stratum corneum is a promising non‐invasive approach for proteomic analysis of skin lesions, but the effects of sampling design and disc pressure on proteome readout remain poorly defined. Here, we assessed how inclusion of surrounding normal‐looking skin and variation in adhesive disc pressure influence protein detection
Ali Azimi +3 more
wiley +1 more source
MxB sensitivity of HIV-1 is determined by a highly variable and dynamic capsid surface
eLife, 2020 The type one interferon induced restriction factor Myxovirus resistance B (MxB) restricts HIV-1 nuclear entry evidenced by inhibition of 2-LTR but not linear forms of viral DNA.
Richard J Miles +5 more
doaj +1 more source
CRISPR/Cas9-Induced Mutagenesis Corroborates the Role of Transportin-SR2 in HIV-1 Nuclear Import
Microbiology Spectrum, 2021 To infect nondividing cells, HIV-1 needs to cross the nuclear membrane. The importin transportin-SR2 (TRN-SR2 or transportin-3) has been proposed to mediate HIV-1 nuclear import, but the detailed mechanism remains unresolved.
Julie Janssens +10 more
doaj +1 more source
Stoichiometric Binding of Cyclophilin‐A to The HIV‐1 Capsid Modulates Its Mechanoelastic Properties
Small, Volume 22, Issue 61, 2 November 2026.The HIV‐1 capsid is a protein nanocapsule that must balance rigidity and flexibility to enter the cell nucleus. Atomic force microscopy simulations reveal that binding of the host protein Cyclophilin A tunes this mechanical response: partial coverage preserves deformability, while excessive binding induces brittleness and failure.
Juan S. Rey +2 more
wiley +1 more source