Results 61 to 70 of about 2,100 (181)
Frontiers in Pharmacology, 2023 Background: 5-Fluorouracil (5-FU) is a widely used chemotherapeutic drug in clinical cancer treatment, including hepatocellular carcinoma (HCC). A correct understanding of the mechanisms leading to a low or lack of sensitivity of HCC to 5-FU-based ...
Xinyu Gu +4 more
doaj +1 more source
CPSF6 deficiency inhibits virus replication.
(A) Immunoblot analyses knocking-out efficiency of CPSF6 in A549 and L929 cells. (B) qRT–PCR analyses of VSV mRNA abundance in WT and CPSF6-/- A549 and L929 cells infected with VSV-eGFP at indicated time points.
Rong Chen (29176) +12 more
core +2 more sources
Advanced Healthcare Materials, EarlyView.This systematic review quantitatively compares conventional mechanical stimulation strategies in cartilage tissue engineering across 85 heterogeneous in vitro studies. Applying standardized effect measures, meta‐analysis reveals that combined compression and shear loading optimally promotes cartilage matrix development.
Jiaqi K. Shen +7 more
wiley +1 more source
MxB sensitivity of HIV-1 is determined by a highly variable and dynamic capsid surface
eLife, 2020 The type one interferon induced restriction factor Myxovirus resistance B (MxB) restricts HIV-1 nuclear entry evidenced by inhibition of 2-LTR but not linear forms of viral DNA.
Richard J Miles +5 more
doaj +1 more source
Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus
Animal Research and One Health, EarlyView.The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary +7 more
wiley +1 more source
In vitro-derived CPSF6-resistant virus may alter CPSF6 binding.
, 2014 (A) HeLa cells stably transduced with the control vector LPCX or one overexpressing CPSF6-358 were infected with VSV-G-pseudotyped GFP reporter viruses. Results are one representative experiment of at least three independent experiments. (B) WT, RKLM and
Matthew S. Henning (507871) +4 more
core +1 more source
The CPSF6-binding interface determines HIV-1 nuclear entry requirements.
, 2012 (A–B) CPSF6 residue F321 is critical for interaction with HIV-1 CAN. (A) HeLa cells expressing empty vector (white bar), HA-tagged CPSF6-358 (black bar) or CPSF6-358 bearing mutation F321N (striped bar) were infected with GFP-encoding VSV-G pseudotyped ...
Vineet N. KewalRamani (138643) +8 more
core +1 more source
CRISPR/Cas9-Induced Mutagenesis Corroborates the Role of Transportin-SR2 in HIV-1 Nuclear Import
Microbiology Spectrum, 2021 To infect nondividing cells, HIV-1 needs to cross the nuclear membrane. The importin transportin-SR2 (TRN-SR2 or transportin-3) has been proposed to mediate HIV-1 nuclear import, but the detailed mechanism remains unresolved.
Julie Janssens +10 more
doaj +1 more source
PROTEOMICS, EarlyView.ABSTRACT Protein S‐palmitoylation, a reversible lipid modification, plays critical roles in regulating protein function and localization. However, its comprehensive role in the rapid reprogramming of macrophages during early immune responses remains incompletely understood.
Hyojung Kim +4 more
wiley +1 more source
Nature Communications, 2020 Early steps of HIV infection of primary human cells remain poorly understood. Here, Francis et al. show that early viral replication complexes accumulate within nuclear speckles, in reliance on viral capsid/host CPSF6 interactions, and preferentially ...
Ashwanth C. Francis +10 more
doaj +1 more source