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Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]
, 2018 Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson +22 more
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BioMed Research International, 2022 CRISPR/Cas9 technology has become the most examined gene editing technology in recent years due to its simple design, yet low cost, high efficiency, and simple operation, which can also achieve simultaneous editing of multiple loci. It can also be carried out without using plasmids, saving lots of troubles caused by plasmids.
openaire +2 more sources
CRISPR/Cas9, a universal tool for genomic engineering
Вавиловский журнал генетики и селекции, 2016 The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we
A. V. Smirnov +3 more
doaj +1 more source
Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish. [PDF]
PLoS ONE, 2017 Although the CRISPR/Cas9 has been successfully applied in zebrafish, considerable variations in efficiency have been observed for different gRNAs. The workload and cost of zebrafish mutant screening is largely dependent on the mutation rate of injected ...
Yunru Chen +9 more
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PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens. [PDF]
, 2017 Large-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool for functional genomics. With its increased popularity, experimental biologists frequently acquire large sequencing datasets for which they often do not have an easy ...
Bath, Tyler +6 more
core +2 more sources
Advancements and Obstacles of CRISPR-Cas9 Technology in Translational Research
Molecular Therapy: Methods & Clinical Development, 2019 The expanding CRISPR-Cas9 technology is an easily accessible, programmable, and precise gene-editing tool with numerous applications, most notably in biomedical research.
Liting You +5 more
doaj +1 more source
mSphere, 2020 Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic,
Timea Marton +3 more
doaj +1 more source
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
Frontiers in Plant Science, 2020 For the past 5 years, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology has appeared in the molecular biology research spotlight.
Yuan Zhang +3 more
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CRISPR/Cas9 system: recent applications in immuno-oncology and cancer immunotherapy
Experimental Hematology & Oncology, 2023 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is essentially an adaptive immunity weapon in prokaryotes against foreign DNA.
Chen Chen, Zehua Wang, Yanru Qin
doaj +1 more source