Results 41 to 50 of about 259,520 (336)

Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a

Scientific Reports, 2022
The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi, Ritsuko Maki, Satsuki Kitano, Hitoshi Miyachi, Fumiko Toyoshima   +4 more
doaj   +1 more source

CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development

Viruses, 2018
The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches.
Arinze Okoli, Malachy I. Okeke, Morten Tryland, Ugo Moens   +3 more
doaj   +1 more source

Prevention of Avian Retrovirus Infection in Chickens Using CRISPR-Cas9 Delivered by Marek’s Disease Virus

Molecular Therapy: Nucleic Acids, 2020
Reticuloendotheliosis virus (REV) is an avian retrovirus that causes an oncogenic, immunosuppressive, and runting-stunting syndrome in avian hosts. The co-infection of REV and Marek’s disease virus (MDV), an oncogenic herpesvirus in chickens, further ...
Kai Li, Yongzhen Liu, Zengkun Xu, Yu Zhang, Yongxiu Yao, Venugopal Nair, Changjun Liu, Yanping Zhang, Yulong Gao, Xiaole Qi, Hongyu Cui, Li Gao, Xiaomei Wang   +12 more
doaj   +1 more source

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source

Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System

Frontiers in Genome Editing, 2022
The nuclease activity of the CRISPR-Cas9 system relies on the delivery of a CRISPR-associated protein 9 (Cas9) and a single guide RNA (sgRNA) against the target gene.
Karim E. Shalaby, Karim E. Shalaby, Mustapha Aouida, Vijay Gupta, Simona S. Ghanem, Omar M. A. El-Agnaf, Omar M. A. El-Agnaf   +6 more
doaj   +1 more source

PinAPL-Py: A comprehensive web-application for the analysis of CRISPR/Cas9 screens. [PDF]

, 2017
Large-scale genetic screens using CRISPR/Cas9 technology have emerged as a major tool for functional genomics. With its increased popularity, experimental biologists frequently acquire large sequencing datasets for which they often do not have an easy ...
Bath, Tyler, Esko, Jeffrey D, Harismendy, Olivier, Kim, Jihoon, Lewis, Nathan E, Spahn, Philipp N, Weiss, Ryan J   +6 more
core   +2 more sources

CRISPR/Cas9-Based Antiviral Strategy: Current Status and the Potential Challenge

Molecules, 2019
From its unexpected discovery as a bacterial adaptive immune system to its countless applications as one of the most versatile gene-editing tools, the CRISPR/Cas9 system has revolutionized every field of life science.
Choongho Lee
doaj   +1 more source

Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides

Genome Biology, 2020
Background CRISPR-Cas9 has been developed as a therapeutic agent for various infectious and genetic diseases. In many clinically relevant applications, constitutively active CRISPR-Cas9 is delivered into human cells without a temporal control system ...
Yan-ru Cui, Shao-jie Wang, Jun Chen, Jie Li, Wenzhang Chen, Shuyue Wang, Bing Meng, Wei Zhu, Zhuhong Zhang, Bei Yang, Biao Jiang, Guang Yang, Peixiang Ma, Jia Liu   +13 more
doaj   +1 more source

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Frontiers in Genetics, 2021
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises
Li Duan, Li Duan, Li Duan, Kan Ouyang, Xiao Xu, Xiao Xu, Limei Xu, Limei Xu, Caining Wen, Caining Wen, Xiaoying Zhou, Xiaoying Zhou, Zhuan Qin, Zhuan Qin, Zhiyi Xu, Zhiyi Xu, Wei Sun, Yujie Liang   +17 more
doaj   +1 more source

Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.). [PDF]

, 2019
Pomegranate (Punica granatum L.) trees are woody perennials that bear colorful and nutritious fruits rich in phenolic metabolites, e.g., hydrolyzable tannins (HTs) and flavonoids.
Chang, Lijing, Tian, Li, Wu, Sheng
core   +1 more source