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CRISPR-Cas systems in prokaryotes [PDF]

Polish Journal of Microbiology, 2015
Prokaryotic organisms possess numerous strategies that enable survival in hostile conditions. Among others, these conditions include the invasion of foreign nucleic acids such as bacteriophages and plasmids.
Burmistrz, Michał, Pyrć, Krzysztof
core   +5 more sources

Advances in Engineering the Fly Genome with the CRISPR-Cas System [PDF]

Genetics, 2017
Drosophila has long been a premier model for the development and application of cutting-edge genetic approaches. The CRISPR-Cas system now adds the ability to manipulate the genome with ease and precision, providing a rich toolbox to interrogate ...
E. Bier, Melissa M. Harrison, Kate M. O’Connor-Giles, Jill Wildonger   +3 more
semanticscholar   +4 more sources

Insight into the natural regulatory mechanisms and clinical applications of the CRISPR-Cas system [PDF]

Heliyon
CRISPR-Cas, the adaptive immune system exclusive to prokaryotes, confers resistance against foreign mobile genetic elements. The CRISPR-Cas system is now being exploited by scientists in a diverse range of genome editing applications.
Hui Cheng, Haoyue Deng, Dongdao Ma, Mengyuan Gao, Zhihan Zhou, Heng Li, Shejuan Liu, Tieshan Teng   +7 more
doaj   +2 more sources

CRISPR-Cas System in Antibiotic Resistance Plasmids in Klebsiella pneumoniae

Frontiers in Microbiology, 2020
CRISPR-Cas (clustered regularly interspersed short palindromic repeats-CRISPR-associated protein) is a microbial adaptive immune system involved in defense against different types of mobile genetic elements.
Muhammad Kamruzzaman, Jonathan R. Iredell, Jonathan R. Iredell   +2 more
doaj   +2 more sources

Prevalence of the CRISPR-cas system and its association with antibiotic resistance in clinical Klebsiella pneumoniae isolates [PDF]

BMC Infectious Diseases
Background and objective(s) CRISPR-Cas is a prokaryotic adaptive immune system that protects bacteria and archaea against mobile genetic elements (MGEs) such as bacteriophages plasmids, and transposons. In this study, we aimed to assess the prevalence of
Hiva Kadkhoda, Pourya Gholizadeh, Reza Ghotaslou, Tahereh Pirzadeh, Mohammad Ahangarzadeh Rezaee, Edris Nabizadeh, Hadi Feizi, Hossein Samadi Kafil, Mohammad Aghazadeh   +8 more
doaj   +2 more sources

Analysis of genetic structure and function of clustered regularly interspaced short palindromic repeats loci in 110 Enterococcus strains

Frontiers in Microbiology, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) are an adaptive immune system involved in specific defenses against the invasion of foreign mobile genetic elements, such as plasmids and phages.
Shuan Tao, Shuan Tao, Dongdong Zhou, Huimin Chen, Na Li, Lin Zheng, Yewei Fang, Yao Xu, Qi Jiang, Wei Liang   +9 more
doaj   +1 more source

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases

Diagnostics, 2022
The CRISPR/Cas system is a protective adaptive immune system against attacks from foreign mobile genetic elements. Since the discovery of the excellent target-specific sequence recognition ability of the CRISPR/Cas system, the CRISPR/Cas system has shown
Junwei Li, Yuexia Wang, Bin Wang, Juan Lou, Peng Ni, Yuefei Jin, Shuaiyin Chen, Guangcai Duan, Rongguang Zhang   +8 more
doaj   +1 more source

An Endogenous Staphylococcus aureus CRISPR-Cas System Limits Phage Proliferation and Is Efficiently Excised from the Genome as Part of the SCCmec Cassette

Microbiology Spectrum, 2023
CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-Cas; however, in the human pathogen Staphylococcus aureus, CRISPR-Cas loci are less common and often studied ...
Kasper Mikkelsen, Janine Zara Bowring, Yong Kai Ng, Frida Svanberg Frisinger, Julie Kjærsgaard Maglegaard, Qiuchun Li, Raphael N. Sieber, Andreas Petersen, Paal Skytt Andersen, Jakob T. Rostøl, Nina Molin Høyland-Kroghsbo, Hanne Ingmer   +11 more
doaj   +1 more source

Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing.

Molecules and Cells, 2021
Compact and versatile CRISPR-Cas systems will enable genome engineering applications through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient miniature Cas system (CasMINI) engineered from the type V-F Cas12f (Cas14 ...
Xiaoshu Xu, Augustine Chemparathy, Leiping Zeng, Hannah R. Kempton, Stephen Shang, Muneaki Nakamura, Lei S. Qi   +6 more
semanticscholar   +1 more source

The Application of the CRISPR-Cas System in Antibiotic Resistance

Infection and Drug Resistance, 2022
The emergence and global epidemic of antimicrobial resistance (AMR) poses a serious threat to global public health in recent years. AMR genes are shared between bacterial pathogens mainly via horizontal gene transfer (HGT) on mobile genetic elements ...
Shuan Tao, Huimin Chen, Na Li, Wei Liang
semanticscholar   +1 more source