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CRISPR-Cas systems in multicellular cyanobacteria [PDF]
RNA Biology, 2018 Novel CRISPR-Cas systems possess substantial potential for genome editing and manipulation of gene expression. The types and numbers of CRISPR-Cas systems vary substantially between different organisms. Some filamentous cyanobacteria harbor > 40 different putative CRISPR repeat-spacer cassettes, while the number of cas gene instances is much lower ...
Shengwei Hou +6 more
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CASPredict: a web service for identifying Cas proteins [PDF]
PeerJ, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated (Cas) proteins constitute the CRISPR-Cas systems, which play a key role in prokaryote adaptive immune system against invasive foreign elements.
Shanshan Yang, Jian Huang, Bifang He
doaj +2 more sources
Mechanisms regulating the CRISPR-Cas systems
Frontiers in Microbiology, 2023 The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats- CRISPR associated proteins) is a prokaryotic system that enables sequence specific recognition and cleavage of nucleic acids. This is possible due to cooperation between CRISPR array which contains short fragments of DNA called spacers that are complimentary to the targeted ...
Marta Zakrzewska +3 more
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Application of the CRISPR/Cas System in Pathogen Detection: A Review
Molecules, 2022 Early and rapid diagnosis of pathogens is important for the prevention and control of epidemic disease. The polymerase chain reaction (PCR) technique requires expensive instrument control, a special test site, complex solution treatment steps and ...
Bowei Yuan +4 more
semanticscholar +1 more source
Carrier strategies boost the application of CRISPR/Cas system in gene therapy
Exploration, 2022 Emerging clustered regularly interspaced short palindromic repeat/associated protein (CRISPR/Cas) genome editing technology shows great potential in gene therapy.
Zunkai Xu +10 more
semanticscholar +1 more source
CRISPR-Cas systems in Proteus mirabilis
Infection, Genetics and Evolution, 2021 The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a bacterial defense mechanism against bacteriophages composed of two different parts: the CRISPR array and the Cas genes. The spacer acquisition is done by the adaptation module consisting of the hallmark Cas1 Cas2 proteins, which inserts new spacers into the CRISPR array.
Mahnaz Shafaei, Fallah +3 more
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Insight into the molecular mechanism of the transposon-encoded type I-F CRISPR-Cas system
Journal of Genetic Engineering and Biotechnology, 2023 CRISPR-Cas9 is a popular gene-editing tool that allows researchers to introduce double-strand breaks to edit parts of the genome. CRISPR-Cas9 system is used more than other gene-editing tools because it is simple and easy to customize.
Amnah Alalmaie +2 more
doaj +1 more source
Frontiers in Plant Science, 2022 Different genome editing approaches have been used to engineer resistance against plant viruses. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas; CRISPR/Cas) systems to create pinpoint genetic ...
Fredy D. A. Silva +1 more
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CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
Frontiers in Cell and Developmental Biology, 2021 The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas).
Nasir Javaid, Sangdun Choi
semanticscholar +1 more source
Using CRISPR-Cas systems as antimicrobials [PDF]
Current Opinion in Microbiology, 2017 Although CRISPR-Cas systems naturally evolved to provide adaptive immunity in bacteria and archaea, Cas nucleases can be co-opted to target chromosomal sequences rather than invasive genetic elements. Although genome editing is the primary outcome of self-targeting using CRISPR-based technologies in eukaryotes, self-targeting by CRISPR is typically ...
Bikard, David, Barrangou, Rodolphe
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