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Recent Advances in Cryo Electron Microscopy and Tomography [PDF]

Structural Dynamics
In the recent years, cryo-electron microscopy (cryo-EM) single-particle analysis (SPA) has greatly benefited from a multitude of technological advances cumulating in breaking the atomic-resolution barrier for biological macromolecules.
Eliza Nieweglowska, Marc M. H. Storms, Matt Joens   +2 more
doaj   +5 more sources

Cryo-electron microscopy-based drug design [PDF]

Frontiers in Molecular Biosciences
Structure-based drug design (SBDD) has gained popularity owing to its ability to develop more potent drugs compared to conventional drug-discovery methods.
Ecenur Cebi, Joohyun Lee, Vinod Kumar Subramani, Nayeon Bak, Changsuk Oh, Kyeong Kyu Kim   +5 more
doaj   +2 more sources

Assembly mechanism of the pleomorphic immature poxvirus scaffold

Nature Communications, 2022
Immature poxviruses are characterized by nonicosahedral semiordered protein scaffolds critical for morphogenesis. Here, the authors use cryo-EM structures of Vaccinia virus D13 scaffold intermediates to explain their assembly mechanism.
Jaekyung Hyun, Hideyuki Matsunami, Tae Gyun Kim, Matthias Wolf   +3 more
doaj   +1 more source

Cryo-electron microscopy structures of capsids and in situ portals of DNA-devoid capsids of human cytomegalovirus

Nature Communications, 2023
The portal-scaffold complex is believed to nucleate the assembly of herpesvirus procapsids. During capsid maturation, two events occur: scaffold expulsion and DNA incorporation. The portal-scaffold interaction and the conformational changes that occur to
Zhihai Li, Jingjing Pang, Rongchao Gao, Qingxia Wang, Maoyan Zhang, Xuekui Yu   +5 more
doaj   +1 more source

Structural insights into RNA bridging between HIV-1 Vif and antiviral factor APOBEC3G

Nature Communications, 2023
Great effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV’s counteraction mechanism to evade antiviral innate immune response.
Takahide Kouno, Satoshi Shibata, Megumi Shigematsu, Jaekyung Hyun, Tae Gyun Kim, Hiroshi Matsuo, Matthias Wolf   +6 more
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COVID-19 serological survey using micro blood sampling

Scientific Reports, 2021
During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of ...
Melissa M. Matthews, Tae Gyun Kim, Satoshi Shibata, Noriko Shibata, Christian Butcher, Jaekyung Hyun, Keon Young Kim, Theodore Robb, Siang Sheng Jheng, Masashi Narita, Tomoari Mori, Mary Collins, Matthias Wolf   +12 more
doaj   +1 more source

Temporal dynamics of charge buildup in cryo-electron microscopy

Journal of Structural Biology: X, 2023
It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation of charge affects imaging parameters and sample stability in transmission electron microscopy is poorly understood.
Makoto Tokoro Schreiber, Alan Maigné, Marco Beleggia, Satoshi Shibata, Matthias Wolf   +4 more
doaj   +1 more source

Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy

Cell Discovery, 2022
Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals. Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical ...
Aojie Wang, Feng Zhou, Congcong Liu, Dongsheng Gao, Ruxi Qi, Yiheng Yin, Sheng Liu, Yuanzhu Gao, Lutang Fu, Yinhe Xia, Yawei Xu, Chuanqing Wang, Zheng Liu   +12 more
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Structural basis for genome packaging, retention, and ejection in human cytomegalovirus

Nature Communications, 2021
Human cytomegalovirus (HCMV) is the prototypical member of the β-herpesvirinae subfamily and the leading viral cause of congenital infections that can lead to birth defects and it can also cause life-threatening disease in immunocompromised individuals ...
Zhihai Li, Jingjing Pang, Lili Dong, Xuekui Yu   +3 more
doaj   +1 more source

Coassembly Generates Peptide Hydrogel with Wound Dressing Material Properties [PDF]

, 2020
Multicomponent self-assembly of peptides is a powerful strategy to fabricate novel functional materials with synergetic properties that can be used for several nanobiotechnological applications.
Berger, Allison A., Böttcher, Christoph, Elomaa, Laura, Koksch, Beate, Nie, Chaunxiong, Thota, Chaitanya Kumar   +5 more
core   +1 more source