Results 11 to 20 of about 132,225 (219)

Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers

Nature Communications, 2023
The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral
Rafael Ayala, Andrey V. Moiseenko, Ting-Hua Chen, Eugene E. Kulikov, Alla K. Golomidova, Philipp S. Orekhov, Maya A. Street, Olga S. Sokolova, Andrey V. Letarov, Matthias Wolf   +9 more
doaj   +1 more source

Measuring the effects of ice thickness on resolution in single particle cryo-EM

Journal of Structural Biology: X, 2023
Ice thickness is a critical parameter in single particle cryo-EM – too thin ice can break during imaging or exclude the sample of interest, while ice that is too thick contributes to more inelastic scattering that precludes obtaining high resolution ...
Kasahun Neselu, Bing Wang, William J. Rice, Clinton S. Potter, Bridget Carragher, Eugene Y.D. Chua   +5 more
doaj   +1 more source

Best practice: setting up and operating a mid-sized cryo-EM facility

Frontiers in Molecular Biosciences, 2023
Ever since the resolution revolution in 2013, cryo-electron microscopy (cryo-EM) has become a powerful methodology in structural biology that is especially suited to study the structure of large flexible molecular complexes.
Xing Meng, Xing Meng, Ishara Ratnayake, Ishara Ratnayake, Martha L. Escobar Galvis, Jason Kotecki, Zack Ramjan, Gongpu Zhao, Gongpu Zhao   +8 more
doaj   +1 more source

Cryo-electron microscopy of viruses [PDF]

, 1984
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions.
A Fukami, Alasdair W. McDowall, AW McDowall, B Cartwright, C Devaux, CA Angell, D Branton, FA Eislering, H Fernandez-Moran, H Söderlund, HP Erickson, J Dubochet, J Dubochet, J Dubochet, J Dubochet, J Lepault, J Lepault, J-J Chang, Jacques Dubochet, Jean Lepault, K Simons, KA Taylor, KE Richards, KR Leonard, L Kääriäinen, LA Amos, Marc Adrian, MF Moody, P Brüggeller, PNT Unwin, PR Smith, SB Hayward, U Aebi, WC Earnshaw, WC Earnshaw, WC Earnshaw   +35 more
core   +1 more source

Cryo-EM map interpretation and protein model-building using iterative map segmentation. [PDF]

, 2020
A procedure for building protein chains into maps produced by single-particle electron cryo-microscopy (cryo-EM) is described. The procedure is similar to the way an experienced structural biologist might analyze a map, focusing first on secondary ...
Afonine PV, Kleywegt GJ, Liebschner D, Terwilliger TC, Terwilliger TC, Terwilliger TC, Terwilliger TC, Terwilliger TC, Terwilliger TC, Terwilliger TC   +9 more
core   +2 more sources

Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography [PDF]

, 2014
Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualised in three-dimensional detail.
Hackstadt T., Louis C., Matsumoto A., Matsumoto A., Newhall W.J., Scidmore M.A., Ward M.E.   +6 more
core   +1 more source

3D ultrastructural organization of whole Chlamydomonas reinhardtii cells studied by nanoscale soft x-ray tomography [PDF]

, 2012
The complex architecture of their structural elements and compartments is a hallmark of eukaryotic cells. The creation of high resolution models of whole cells has been limited by the relatively low resolution of conventional light microscopes and the ...
Frangakis, Achilleas S., Guttmann, Peter, Hummel, Eric, Kunz, Michael, Schneider, Gerd, Tarek, Basel, Werner, Stephan, Westermann, Benedikt   +7 more
core   +10 more sources

Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes

Cell Reports, 2021
Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang, Matthew J. Belousoff, Yi-Lynn Liang, Radostin Danev, Patrick M. Sexton, Denise Wootten   +5 more
doaj   +1 more source

`Cryo-EM': electron cryomicroscopy, cryo electron microscopy or something else?

IUCrJ, 2023
Structural biology continues to benefit from an expanding toolkit, which is helping to gain unprecedented insight into the assembly and organization of multi-protein machineries, enzyme mechanisms and ligand/inhibitor binding.
Richard Henderson, Samar Hasnain
doaj   +1 more source

A saposin-lipoprotein nanoparticle system for membrane proteins. [PDF]

, 2016
A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with ...
Armache, Jean-Paul, Briggs, John AG, Cheng, Yifan, Flayhan, Ali, Frauenfeld, Jens, Garoff, Henrik, Guettou, Fatma, Jegerschöld, Caroline, Löving, Robin, Löw, Christian, Moberg, Per, Nordlund, Pär, Sonnen, Andreas F-P, Zhu, Lin   +13 more
core   +2 more sources