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Bringing Structure to Cell Biology with Cryo-Electron Tomography [PDF]
Annual Review of Biophysics, 2023 Recent advances in cryo-electron microscopy have marked only the beginning of the potential of this technique. To bring structure into cell biology, the modality of cryo-electron tomography has fast developed into a bona fide in situ structural biology ...
Lindsey N Young, Elizabeth Villa
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Cryo-electron tomography of enveloped viruses
Trends in Biochemical Sciences, 2022Viruses are macromolecular machineries that hijack cellular metabolism for replication. Enveloped viruses comprise a large variety of RNA and DNA viruses, many of which are notorious human or animal pathogens. Despite their importance, the presence of lipid bilayers in their assembly has made most enveloped viruses too pleomorphic to be reconstructed ...
Sai Li
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Statistical spatial analysis for cryo-electron tomography
Computer Methods and Programs in Biomedicine, 2022 Cryo-electron tomography (cryo-ET) is uniquely suited to precisely localize macromolecular complexes in situ, that is in a close-to-native state within their cellular compartments, in three-dimensions at high resolution. Point pattern analysis (PPA) allows quantitative characterization of the spatial organization of particles.
Wolfgang Baumeister +2 more
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On geometric artifacts in cryo electron tomography
Ultramicroscopy, 2016Single-tilt scheme is nowadays the prevalent acquisition geometry in electron tomography and subtomogram averaging experiments. Being an incomplete scheme that induces ill-posedness in the sense of the X-ray or Radon transform inverse problem, it introduces a number of artifacts that directly influence the quality of tomographic reconstructions. Though
Turoňová, B. +2 more
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Cryo-electron Tomography of Neurospora Mitochondria
Journal of Structural Biology, 2000Cryo-electron tomography was used to study the structural organization of whole frozen-hydrated mitochondria from Neurospora crassa. Unlike mitochondria from many other species and tissues, in this case the cristae form a three-dimensional network of interconnected lamellae.
Nicastro, D. +3 more
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Smart parallel automated cryo-electron tomography
Nature Methods, 2023Abstract In situ cryo electron tomography enables investigation of macromolecules in their native cellular environment. Data collection, however, requires an experienced operator and valuable microscope time to carefully select targets for tilt series acquisition.
Fabian Eisenstein +2 more
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Cryo‐electron microscopy and cryo‐electron tomography of nanoparticles
WIREs Nanomedicine and Nanobiotechnology, 2016Cryo‐transmission electron microscopy (cryo‐TEM or cryo‐EM) and cryo‐electron tomography (cryo‐ET) offer robust and powerful ways to visualize nanoparticles. These techniques involve imaging of the sample in a frozen‐hydrated state, allowing visualization of nanoparticles essentially as they exist in solution. Cryo‐TEM grid preparation can be performed
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2019
Classical structural biology approaches rely on highly purified molecules which are isolated from their neighbors far from the complex macromolecular interaction network of the cell (ex situ). We have seen breathtaking results of such isolated molecular structures at atomic or near-atomic resolution obtained by single-particle cryo-electron microscopy (
Jürgen Plitzko, Wolfgang P. Baumeister
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Classical structural biology approaches rely on highly purified molecules which are isolated from their neighbors far from the complex macromolecular interaction network of the cell (ex situ). We have seen breathtaking results of such isolated molecular structures at atomic or near-atomic resolution obtained by single-particle cryo-electron microscopy (
Jürgen Plitzko, Wolfgang P. Baumeister
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Cryo-electron tomography in biology and medicine
Annals of Anatomy - Anatomischer Anzeiger, 2009During the last six decades electron microscopy (EM) has been essential to ultra-structural studies of the cell to understand the fundamentals of cellular morphology and processes underlying diseases. More recently, electron tomography (ET) has emerged as a novel approach able to provide three-dimensional (3D) information on cells and tissues at ...
Roman I, Koning, Abraham J, Koster
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