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Cellular Cryo-Electron Tomography
2023Electron tomography at cryogenic temperatures is becoming a prominent tool in cell biology. It provides three-dimensional detailed information on cellular organization. Advanced image processing of tomogram sub-volumes allows near-atomic in-cell macromolecules structure determination.
Judernatz, Jo H. +2 more
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Alignment of Cryo-Electron Tomography Datasets
2010Data acquisition of cryo-electron tomography (CET) samples described in previous chapters involves relatively imprecise mechanical motions: the tilt series has shifts, rotations, and several other distortions between projections. Alignment is the procedure of correcting for these effects in each image and requires the estimation of a projection model ...
Fernando, Amat +5 more
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Cellular Nanoimaging by Cryo Electron Tomography
2012Cryo electron tomography is a technique that allows visualization of biological specimens in three dimensions with nanometer resolution. For cryo immobilized life sciences samples it can reveal cellular morphology, the shape of membranous structures, and depict internal macromolecular arrangements and large proteins.
Roman I, Koning, Abraham J, Koster
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Dynamics of antibodies from cryo-electron tomography
Biophysical Chemistry, 2005The issue of protein dynamics and its implications in the biological function of proteins are arousing greater and greater interest in molecular biology. In cryo-electron tomography experiments one takes several snapshots of a given biological macromolecule.
L. Bongini +5 more
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Zernike phase contrast cryo-electron tomography
Journal of Structural Biology, 2010Cryo-tomography in the electron microscope is unique in its ability to provide high-resolution, three-dimensional structural information about cells, organelles and macromolecules in a nearly native, frozen-hydrated state. However, the phase-contrast imaging method used in conventional cryo-electron tomography fails to faithfully represent the full ...
R. Danev +3 more
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Getting Started with In Situ Cryo-Electron Tomography
2020Cryo-electron tomography (cryo-ET) is an extremely powerful tool which is used to image cellular features in their close-to-native environment at a resolution where both protein structure and membrane morphology can be revealed. Compared to conventional electron microscopy methods for biology, cryo-ET does not include the use of potentially artifact ...
Daniel, Serwas, Karen M, Davies
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Cryo-electron tomography of nanoparticle transmigration into liposome
Journal of Structural Biology, 2009Nanoparticle transport across cell membrane plays a crucial role in the development of drug delivery systems as well as in the toxicity response induced by nanoparticles. As hydrophilic nanoparticles interact with lipid membranes and are able to induce membrane perturbations, hypothetic mechanisms based on membrane curvature or hole formation have been
Le Bihan, Olivier +9 more
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Cryo-Electron Tomography of Cellular Microtubules
2010Microtubules are intrinsically dynamic structures. In the cellular environment many proteins and protein complexes are associated with microtubules that influence or functionalize microtubule dynamics. Therefore, investigation of the structure and dynamics of microtubules with their associated complexes inside the cellular environment lies at the heart
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Labeling systems for cryo-electron tomography
Current Opinion in Structural BiologyThe unrealized goal of cryo-electron tomography (cryo-ET) is to visualize every protein within its cellular context. Such capability would enable molecular resolution mapping of three-dimensional protein topography and structure determination within a native context.
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Cryo-Electron Microscopy and Cryo-Electron Tomography of Viruses
2023Daniel Luque, José R. Castón
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