Results 91 to 100 of about 14,888,742 (175)

Cryo-mesh: a simple alternative cryopreservation protocol

, 2017
BACKGROUND: The continued development of new cryopreservation protocols has improved post-cryogenic success rates for a wide variety of plant species.
E. Bunn (23318704), Bryn Funnekotter (23289208), Ricardo Mancera (23286790)   +2 more
core  

Cryo-EM data collection, refinement and validation statistics.

, 2020
Cryo-EM data collection, refinement and validation statistics.
Jörg Bürger (302968), Michael Kolbe (211045), Michele Lunelli (211042), Christian M. T. Spahn (5533475), Antje Kamprad (8486658), Thorsten Mielke (232927)   +5 more
core   +1 more source

Ultrafast Isolation of Synaptic Terminals From Rat Brain for Cryo-Electron Tomography Analysis

Bio-Protocol
Understanding the nanoscale organization and molecular rearrangement of synaptic components is critical for elucidating the mechanisms of synaptic transmission and plasticity.
Rong Sun, Qiangjun Zhou
doaj   +1 more source

Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling

Nature Communications
Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following fixation, staining, and sectioning, which limit resolution and introduce artifacts.
Benjamin C. Creekmore, Kathryn Kixmoeller, Ben E. Black, Edward B. Lee, Yi-Wei Chang   +4 more
doaj   +1 more source

Cryo-ET reveals a novel ATPase complex structure in B. burgdorferi.

, 2018
(A) A central section of a flagellar motor structure from WT cells. The structure was generated after alignment of the ATPase complex region, classification of the spoke region (see S2 Fig), and image refinement on the C-ring and ATPase complex region ...
Zhuan Qin (5949530), Jiagang Tu (342161), Md A. Motaleb (5949536), Tao Lin (124891), Chunhao Li (5949533), Jun Liu (42548), Steven J. Norris (124904)   +6 more
core   +1 more source

Latest developments in accurate and high-throughput correlative cryo-FIB milling for cryo-ET lamella production [PDF]

BIO Web of Conferences
Smeets Marit, Daviran Deniz, Teunisse Wessel   +2 more
doaj   +1 more source

Novel combination of integrated FLM with cryo-FIB allows targeted cryo-ET sample preparation from challenging samples [PDF]

BIO Web of Conferences
Smeets Marit, Zachej Samuel, Teunisse Wessel, Javůrek Jakub, Pinkas Dominik, Filimonenko Vlada   +5 more
doaj   +1 more source

In-cell cryo-electron tomography reveals differential effects of type I and type II kinase inhibitors on LRRK2 filament formation and microtubule association

eLife
Mutations in leucine-rich repeat kinase 2 (LRRK2) are a leading contributor to developing familial and idiopathic Parkinson’s disease (PD). Most PD-causing LRRK2 mutations increase the kinase activity, leading to increased phosphorylation of Rab GTPases,
Tamar Basiashvili, Joshua Hutchings, Siyu Chen, Eva P Karasmanis, William Alexander Flaherty, Andres E Leschziner, Elizabeth Villa   +6 more
doaj   +1 more source

Getting Started in Cryo-EM

, 2015
These video lectures by Professor Grant Jensen cover the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image ...
Jensen, Grant J.
core   +1 more source

Nanoscale analysis of human G1 and metaphase chromatin in situ

The EMBO Journal
The structure of chromatin at the nucleosome level inside cells is still incompletely understood. Here we present in situ electron cryotomography analyses of chromatin in both G1 and metaphase RPE-1 cells.
Jon Ken Chen, Tingsheng Liu, Shujun Cai, Weimei Ruan, Cai Tong Ng, Jian Shi, Uttam Surana, Lu Gan   +7 more
doaj   +1 more source