Peptide Conjugation via CuAAC ‘Click’ Chemistry [PDF]
Molecules, 2013 The copper (I)-catalyzed alkyne azide 1,3-dipolar cycloaddition (CuAAC) or ‘click’ reaction, is a highly versatile reaction that can be performed under a variety of reaction conditions including various solvents, a wide pH and temperature range, and ...
Abdullah A H Ahmad Fuaad +2 more
exaly +8 more sources
Atroposelective interrupted CuAAC reaction using cyclic diaryliodoniums [PDF]
Nature CommunicationsCopper-catalyzed azide–alkyne cycloaddition (CuAAC) is a pivotal strategy for joining two fragments, including bioactive moieties under mild conditions.
Yuanyuan Li +3 more
doaj +4 more sources
A CuAAC–Hydrazone–CuAAC Trifunctional Scaffold for the Solid-Phase Synthesis of Trimodal Compounds: Possibilities and Limitations [PDF]
Molecules, 2015 We present a trifunctional scaffold designed for the solid-phase synthesis of trimodal compounds. This scaffold holds two alkyne arms in a free and TIPS-protected form for consecutive CuAAC (copper(I)-catalyzed azide–alkyne cycloaddition), one Fmoc ...
Benjamin Fabre +4 more
doaj +3 more sources
A flow platform for degradation-free CuAAC bioconjugation [PDF]
Nature Communications, 2018 Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is a common bioconjugation technique; however oxidative degradation and residual copper may limit its use.
Marine Z. C. Hatit +5 more
doaj +7 more sources
Pathway selection between click and acyl transfer reactions driven by aminoacyl phosphates [PDF]
Nature CommunicationsCovalent transformations in biology follow defined temporal sequences that regulate processes such as acylation and phosphorylation, yet achieving comparable temporal control in synthetic systems remains challenging.
Debjyoti Bhattacharjee +6 more
doaj +2 more sources
Site-specific bioconjugation of a murine dihydrofolate reductase enzyme by copper(I)-catalyzed azide-alkyne cycloaddition with retained activity. [PDF]
PLoS ONE, 2014 Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) is an efficient reaction linking an azido and an alkynyl group in the presence of copper catalyst. Incorporation of a non-natural amino acid (NAA) containing either an azido or an alkynyl group into a ...
Sung In Lim +4 more
doaj +6 more sources
Molecules, 2016 The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule labeling and detecting. Of all the bioorthogonal reactions that have been developed, the copper-catalyzed azide-alkyne cycloaddition (CuAAC) is the most widely ...
Zhiyuan Zhang
exaly +3 more sources
Held out wings RNA binding activity in the cytoplasm during early spermatogenesis [PDF]
Communications BiologyHeld out wings (HOW) is an RNA-binding protein essential for spermatogenesis in Drosophila melanogaster. HOW is a signal transduction and activation of RNA (STAR) protein, regulating post-transcriptional gene expression.
Michaela Agapiou +7 more
doaj +2 more sources
Intermediates Stabilized by Tris(triazolylmethyl)amines in the CuAAC Reaction
Chemistry - A European Journal, 2017 AbstractTris(triazolylmethyl)amine ligands (TL) are widely used to accelerate the CuI‐catalyzed azide‐alkyne cycloaddition (CuAAC) reaction, but its mechanistic role remains unclear. Using electrospray ionization mass spectrometry, we detected for the first time the trinuclear TL‐CuI3‐acetylide and the dinuclear TL‐CuI2‐acetylide complexes in aqueous ...
Haoqing Chen, Chengzhi Cai, Siheng Li
exaly +4 more sources
AT-CuAAC Synthesis of Mechanically Interlocked Oligonucleotides [PDF]
Journal of the American Chemical Society, 2020 We present a simple strategy for the synthesis of main chain oligonucleotide rotaxanes with precise control over the position of the macrocycle. The novel DNA-based rotaxanes were analyzed to assess the effect of the mechanical bond on their properties.
Amanda Acevedo-Jake +7 more
openaire +5 more sources