Peptide Conjugation via CuAAC ‘Click’ Chemistry
Molecules, 2013 The copper (I)-catalyzed alkyne azide 1,3-dipolar cycloaddition (CuAAC) or ‘click’ reaction, is a highly versatile reaction that can be performed under a variety of reaction conditions including various solvents, a wide pH and temperature range, and ...
Mariusz Skwarczynski +2 more
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Molecules, 2016 The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule labeling and detecting. Of all the bioorthogonal reactions that have been developed, the copper-catalyzed azide-alkyne cycloaddition (CuAAC) is the most widely ...
Zhiyuan Zhang
exaly +4 more sources
1,2,3-Triazole-linked DNA biosensors: molecular design to real-time applications [PDF]
RSC AdvancesMany aspects of modern life rely on rapid, accurate, and selective detection methodologies, especially for heavy toxins, pathogens, biomolecules, metal ions, and miRNAs.
Gaurav Bhatoe +4 more
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Advancements and applications of click chemistry in protein labeling and bioconjugation [PDF]
RSC AdvancesClick chemistry has emerged as a versatile and efficient chemical strategy for constructing complex molecules under mild conditions. Its core reaction is copper(i)-catalyzed azide–alkyne cycloaddition (CuAAC), which provides high yields ...
Usman Nazeer, Guoting Qin, Chengzhi Cai
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A SPAAC-Based Bioorthogonal Method for Verifying Protein Palmitoylation [PDF]
Bio-ProtocolPalmitoylation is a crucial post-translational modification, and bioorthogonal chemistry based on azide-alkyne cycloaddition is typically used to verify protein palmitoylation.
Yaoyu Zhang +6 more
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Pathway selection between click and acyl transfer reactions driven by aminoacyl phosphates [PDF]
Nature CommunicationsCovalent transformations in biology follow defined temporal sequences that regulate processes such as acylation and phosphorylation, yet achieving comparable temporal control in synthetic systems remains challenging.
Debjyoti Bhattacharjee +6 more
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Catalytic Enantioselective Construction of Planar Chiral Heterocyclic [2.2]Paracyclophanes Enabled by Copper-Catalyzed Desymmetric Azide-Alkyne Cycloaddition [PDF]
Nature CommunicationsThe construction of planar chiral [2.2]paracyclophanes (PCPs) is of great importance due to their intriguing photophysical and optoelectronic properties.
Zhuang-Zhuang Li +2 more
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Atroposelective interrupted CuAAC reaction using cyclic diaryliodoniums [PDF]
Nature CommunicationsCopper-catalyzed azide–alkyne cycloaddition (CuAAC) is a pivotal strategy for joining two fragments, including bioactive moieties under mild conditions.
Yuanyuan Li +3 more
doaj +2 more sources
Novel CuCLIP-seq for in situ covalently captured protein-binding RNAs [PDF]
Nature CommunicationsRNA binding proteins (RBPs) are multi-faceted proteins that interact with transcripts in various RNA driven processes and functions. However, in situ covalent capture techniques for screening authentic RNA substrates of RBPs remain challenging in terms ...
Xing Wang +11 more
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Click chemistry as a promising protocol for fluorogenic reactions [PDF]
Records of Pharmaceutical & Biomedical Sciences, 2023 The Nobel prize in chemistry has been awarded to the click chemistry approach in 2022 due to its significant impact on nearly all fields of chemical science.
Heba Aref +4 more
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