Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag. [PDF]
PLoS ONE, 2021 Epigenome research has employed various methods to identify the genomic location of proteins of interest, such as transcription factors and histone modifications.
Yasuhiro Fujiwara +5 more
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Multi-CUT&Tag to simultaneously profile multiple chromatin factors [PDF]
STAR Protocols, 2022 Summary: Genome-wide chromatin mapping approaches typically focus on one protein at a time. We recently developed multi-CUT&Tag, which enables simultaneous mapping of multiple chromatin proteins in the same single cells or pools of cells.
Sneha Gopalan, Thomas G. Fazzio
doaj +2 more sources
GoPeaks: histone modification peak calling for CUT&Tag [PDF]
Genome Biology, 2022 Genome-wide mapping of histone modifications is critical to understanding transcriptional regulation. CUT&Tag is a new method for profiling histone modifications, offering improved sensitivity and decreased cost compared with ChIP-seq.
William M. Yashar +8 more
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CUT&Tag for High‐Resolution Epigenomic Profiling From a Low Amount of Arabidopsis Tissue [PDF]
Plant DirectBackground The genome‐wide profiling of chromatin states that are defined by different histone posttranslational modifications, known as epigenomic profiling, is crucial for understanding the epigenetic regulations of gene expression, both in animal and ...
Yixuan Fu +2 more
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Epigenomic analysis of formalin-fixed paraffin-embedded samples by CUT&Tag [PDF]
Nature Communications, 2023 For more than a century, formalin-fixed paraffin-embedded (FFPE) sample preparation has been the preferred method for long-term preservation of biological material.
Steven Henikoff +8 more
doaj +2 more sources
Rapid and Low-Input Profiling of Histone Marks in Plants Using Nucleus CUT&Tag [PDF]
Frontiers in Plant Science, 2021 Characterizing genome-wide histone posttranscriptional modifications and transcriptional factor occupancy is crucial for deciphering their biological functions.
Weizhi Ouyang +8 more
doaj +2 more sources
Efficient chromatin profiling of H3K4me3 modification in cotton using CUT&Tag [PDF]
Plant Methods, 2020 Background In 2019, Kaya-Okur et al. reported on the cleavage under targets and tagmentation (CUT&Tag) technology for efficient profiling of epigenetically modified DNA fragments.
Xiaoyuan Tao +3 more
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Af-CUT&Tag: a sensitive and antibody-free chromatin profiling method using genetically encoded tags and high-affinity binders fused to Tn5 [PDF]
Nature CommunicationsConventional chromatin profiling techniques are often limited by antibody availability and performance. Here, we introduce Af-CUT&Tag, a target antibody-free method that overcomes these limitations by using CRISPR-integrated peptide tags (HiBiT/ALFA-tag)
Xindong Wang +14 more
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CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks [PDF]
Nature CommunicationsDNA-protein interactions have traditionally been profiled via chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq). Cleavage Under Targets & Tagmentation (CUT&Tag) is a rapidly expanding technique that enables the profiling of ...
Leyla Abbasova +7 more
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CUT&Tag and DiBioCUT&Tag enable investigation of the AT-rich epigenome of Plasmodium falciparum from low-input samples [PDF]
Cell Reports: MethodsSummary: Phenotypic variation between malaria parasites is a major contributor to the pathogen’s success, facilitated by heritable yet dynamic changes in (hetero)chromatin structure.
Jonas Gockel +4 more
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