Cut&tag: a powerful epigenetic tool for chromatin profiling [PDF]
Epigenetics, 2023 Analysis of transcription factors and chromatin modifications at the genome-wide level provides insights into gene regulatory processes, such as transcription, cell differentiation and cellular response.
Zhijun Fu +5 more
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Genome-wide profiling of histone H3K4me3 and H3K27me3 modifications in individual blastocysts by CUT&Tag without a solid support (NON-TiE-UP CUT&Tag) [PDF]
Scientific Reports, 2022 Individual analysis of the epigenome of preimplantation embryos is useful for characterizing each embryo and for investigating the effects of environmental factors on their epigenome.
Kazuki Susami +4 more
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CUT&Tag reveals unconventional G-quadruplex landscape in Mycobacterium tuberculosis in response to oxidative stress [PDF]
Nature CommunicationsMycobacterium tuberculosis (Mtb), the causative agent of tuberculosis, remains a global health threat due to increasing drug resistance and high mortality rates. To combat tuberculosis effectively, novel therapeutic targets are urgently needed.
Ilaria Maurizio +6 more
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YAP-mediated mechanotransduction in urinary bladder remodeling: Based on RNA-seq and CUT&Tag [PDF]
Frontiers in Genetics, 2023 Yes-associated protein (YAP) is an important transcriptional coactivator binding to transcriptional factors that engage in many downstream gene transcription. Partial bladder outlet obstruction (pBOO) causes a massive burden to patients and finally leads
Xingpeng Di +3 more
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Low-input CUT&Tag for efficient epigenomic profiling of zebrafish stage I oocytes [PDF]
Frontiers in Cell and Developmental BiologyHistone modification signatures mark sites of transcriptional regulatory elements and regions of gene activation and repression. These sites vary among cell types and undergo dynamic changes during development and in diseases.
Qianwen Zheng +5 more
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CUT&Tag for efficient epigenomic profiling of small samples and single cells [PDF]
Nature Communications, 2019 Understanding gene regulation will require mapping specific chromain features in a small number of cells at high resolution. Here the authors describe CUT&Tag, which uses antibody-mediated tethering of Tn5 transposase to a chromatin protein to generate ...
Hatice S. Kaya-Okur +7 more
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Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag [PDF]
STAR Protocols, 2021 Summary: Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury.
Yuefeng Li +11 more
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H3K4me3 CUT&Tag and Transcriptome Analysis Reveal the Epigenetic Regulatory Landscape in Mammary Gland Tissues of Yili Horses at Different Lactation Stages [PDF]
AnimalsH3K4me3, a well-established histone modification associated with active promoters, plays a critical role in orchestrating gene expression programs that govern mammary gland development and lactation.
Lingling Liu +4 more
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fCUT&Tag‐Seq: An optimized CUT&Tag‐based method for high‐resolution profiling of histone modifications and chromatin‐binding proteins in fungi [PDF]
mLifeHistone modifications and chromatin‐binding proteins play crucial roles in regulating gene expression in eukaryotes, with significant implications for fungal pathogenicity and development.
Haiting Wang +11 more
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CUT&Tag applied to zebrafish adult tail fins reveals a return of embryonic H3K4me3 patterns during regeneration [PDF]
Epigenetics & ChromatinRegenerative potential is governed by a complex process of transcriptional reprogramming, involving chromatin reorganization and dynamics in transcription factor binding patterns throughout the genome. The degree to which chromatin and epigenetic changes
Phu Duong +3 more
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