Commentary on 'Untargeted CUT&Tag reads are enriched at accessible chromatin and restrict identification of potential G4-forming sequences in G4-targeted CUT&Tag experiments'. [PDF]
Nucleic Acids ResIn a recent study (Nucleic Acids Research, Volume 53, Issue 14, 12 August 2025, gkaf678), Thompson and Byrd examine potential issues in the mapping of DNA G-quadruplexes (G4s) by CUT&Tag and related methods [1]. The authors conclude that detection of G4s in chromatin is significantly confounded by artefacts arising from background Tn5 enzymatic ...
Melidis L +5 more
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Automated CUT&Tag profiling of chromatin heterogeneity in mixed-lineage leukemia. [PDF]
Nat Genet, 2021 Abstract Acute myeloid and lymphoid leukemias often harbor chromosomal translocations involving the KMT2A gene, encoding the KMT2A lysine methyltransferase (also known as mixed-lineage leukemia-1), and produce in-frame fusions of KMT2A to other chromatin-regulatory proteins.
Janssens DH +7 more
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Untargeted CUT&Tag reads are enriched at accessible chromatin and restrict identification of potential G4-forming sequences in G4-targeted CUT&Tag experiments. [PDF]
Nucleic Acids ResAbstract G-quadruplex DNA structures (G4s) form within single-stranded DNA in nucleosome-free chromatin. G4s modulate gene expression and genomic stability, so high-throughput, genome-wide mapping of G4s has generated strong research interest and methodological innovation. Recently, the Cleavage Under Targets and Tagmentation (CUT&
Thompson MD, Byrd AK.
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The development and application of cleavage under targets and tagmentation (CUT&Tag) technology. [PDF]
J Biol MethodsThe regulation of chromatin structure and gene transcription in eukaryotic cells is an involved process mediated by histone modifications, chromatin-binding proteins, and transcription factors. Studying the distribution of histone modifications and transcription factors at the whole-genome level is crucial for understanding the mechanisms of gene ...
Xiong C, Wang J, Li X, Li G, Wang X.
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Genome-wide mapping of G-quadruplex structures with CUT&Tag. [PDF]
Nucleic Acids Res, 2022 Abstract Single-stranded genomic DNA can fold into G-quadruplex (G4) structures or form DNA:RNA hybrids (R loops). Recent evidence suggests that such non-canonical DNA structures affect gene expression, DNA methylation, replication fork progression and genome stability. When and how G4 structures form and are resolved remains unclear.
Lyu J +3 more
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Tn5 DNA Transposase in Multi-Omics Research
Methods and Protocols, 2023 Tn5 transposase use in biotechnology has substantially advanced the sequencing applications of genome-wide analysis of cells. This is mainly due to the ability of Tn5 transposase to efficiently transpose DNA essentially randomly into any target DNA ...
Dmitry Penkov +2 more
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Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
Bio-Protocol, 2021 We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and ...
Steven Henikoff +2 more
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Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation
eLife, 2020 Chromatin accessibility mapping is a powerful approach to identify potential regulatory elements. A popular example is ATAC-seq, whereby Tn5 transposase inserts sequencing adapters into accessible DNA (‘tagmentation’).
Steven Henikoff +3 more
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Efficient low-cost chromatin profiling with CUT&Tag. [PDF]
Nat Protoc, 2020 We recently introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling strategy in which antibodies are bound to chromatin proteins in situ in permeabilized nuclei. These antibodies are then used to tether the cut-and-paste transposase Tn5.
Kaya-Okur HS +4 more
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Review and Evaluate the Bioinformatics Analysis Strategies of ATAC-seq and CUT&Tag Data. [PDF]
Genomics Proteomics BioinformaticsAbstract Efficient and reliable profiling methods are essential to study epigenetics. Tn5, one of the first identified prokaryotic transposases with high DNA-binding and tagmentation efficiency, is widely adopted in different genomic and epigenomic protocols for high-throughputly exploring the genome and epigenome.
Cheng S, Miao B, Li T, Zhao G, Zhang B.
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