Results 161 to 170 of about 2,736 (203)

Deciphering external chain length and cyclodextrin production with starch catalyzed by cyclodextrin glycosyltransferase

Carbohydrate Polymers, 2022
Cyclodextrins (CDs) are yielded by cyclodextrin glycosyltransferase (CGTase) using starches or their derivatives as substrates. However, how starch fine structure affects it production is still ambiguous. This study aimed to decipher the relevance between the external chain length (ECL) and CD production.
Hangyan, Ji, Yuxiang, Bai, Yixi, Liu, Yanli, Wang, Xiaobei, Zhan, Jie, Long, Long, Chen, Chao, Qiu, Zhengyu, Jin   +8 more
openaire   +2 more sources

Interference of Cyclodextrins with Amylolytic Activity Assays of Cyclodextrin Glycosyltransferases

Starch - Stärke, 1997
AbstractCyclodextrin glycosyltransferases (CGTases) catalyse the cyclisation of starch dextrins to cyclodextrins and concomitantly show amylolytic activity which is commonly determined by the starch‐iodine assay. In the present study it was found that cyclodextrins interfere with this assay. Adding cyclodextrins in concentrations ranging from 0.01mM to
Lars Haastrup Pedersen, Hans J. S. Christensen, Flemming Mathiesen, Kim L. Larsen, Wolfgang Zimmermann   +4 more
openaire   +3 more sources

Specific alpha-cyclodextrin production by a novel thermostable cyclodextrin glycosyltransferase

Applied Microbiology and Biotechnology, 1988
A novel bacterial isolate, Bacillus amyloliquefaciens strain AL 35, produced high yields of a cyclodextrin glycosyltransferase (CGT'ase) when grown in a submerged culture. The stability of CGT'ase to high temperature and alkaline pH enabled processing for cyclodextrin production to be carried out at 60° C and pH 9.0.
Ernest K. C. Yu, Hiroyuki Aoki, Masanaru Misawa   +2 more
openaire   +1 more source

Study of cyclodextrin production using cyclodextrin glycosyltransferase immobilized on chitosan

Journal of Chemical Technology & Biotechnology, 1989
AbstractCyclodextrin glycosyltransferase (CGTase EC.3.2.1.19) was immobilized on chitosan through covalent bonding using glutaratdehyde. Various characteristics of immobilized CGTase (ICGTase) such as the pH—activity curve, the temperature—activity curve, pH and thermal stability, batch re‐usability and continuous operation stability were evaluated ...
Chin‐Ping Yang, Chein‐Shyong Su
openaire   +1 more source

Cyclodextrins are likely to induce cyclodextrin glycosyltransferase production inBacillus macerans

Folia Microbiologica, 1998
Cyclodextrin glycosyltransferase (CGTase) activity was monitored inBacillus macerans culture fluids up to 56 h incubation time using glucose (G1), maltose (G2), maltotriose (G3), maltoheptaose (G7), α-,β-,γ-cyclodextrins (CDs) and soluble starch as carbon sources. Highest maximum specific growth rates (µmax) were observed with glucose, γ-CD and soluble
I. Pócsi, N. Nógrády, A. Lipták, A. Szentirmai   +3 more
openaire   +1 more source

Chain Length Specifity of Cyclodextrin Glycosyltransferase

Starch - Stärke, 1992
AbstractBacillus macerans CGTase recognizes the terminal 6 glucose units of the non‐reducing end of α‐(1→4)‐glucans. Analysis of the chain‐length distributions produced by the action of CGTase on well‐defined maltodextrins gave information on the enzyme's specifity in regard of the size of the transferred glucanosyl‐moiety.It was found that in the ...
Dirk Vetter, Werner Thorn
openaire   +1 more source

BIOPROSPECTING FOR CYCLODEXTRIN GLYCOSYLTRANSFERASE PRODUCING BACTERIA

INDIAN DRUGS, 2014
Cyclodextrin glycosyltransferase- (CGTase), (EC.2.4.1.19) producing micro-organisms were isolated from different sources of soil (paddy plantation, potato plantation, garden and Malpe beach) from places in and around Udupi, India. A potential isolate MBS 24 was identified as Virgibacillus pantothenticus.
Y. Rajesh, K Narayanan, V. M. Subrahmanyam, J. Venkata Rao   +3 more
openaire   +1 more source

Purification of Cyclodextrin Glycosyltransferase by Immunochromatography

Starch - Stärke, 1997
AbstractCyclodextrin glycosyltransferase (CGTase) was produced in recombinant Escherichia coli which contains the cgt gene of Bacillus macerans in the pTCGT1 plasmid. Antiserum against CGTase was prepared in rabbits. Anti‐CGTase IgG in Serum was purified using an immobilized Protein A affinity column and an immobilized E.
Nam Soo Han, Bernard Y. Tao
openaire   +1 more source

Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase.

European journal of biochemistry, 2003
Cyclodextrin glycosyltransferase (CGTase) uses an alpha-retaining double displacement mechanism to catalyze three distinct transglycosylation reactions. To investigate these reactions as catalyzed by the CGTase from Thermoanaerobacterium thermosulfurigenes the enzyme was overproduced (8 mg.L(-1) culture) using Bacillus subtilis as a host.
Hans, Leemhuis, Bauke W, Dijkstra, Lubbert, Dijkhuizen   +2 more
openaire   +1 more source

Biochemical properties and cyclodextrin production profiles of isoforms of cyclodextrin glycosyltransferase

Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2010
Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch to cyclodextrin (CD), an important host molecule for the study of host–guest interactions. CGTase from Paenibacillus sp. RB01 and its recombinant form showed the same isoform pattern.
Wanchai Yenpetch, Kanoktip Packdibamrung, Wolfgang Zimmermann, Piamsook Pongsawasdi   +3 more
openaire   +1 more source