Results 11 to 20 of about 2,736 (203)

Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production [PDF]

BMC Biotechnology, 2018
Background Cyclodextrin glucanotransferase (CGTase) can transform L-ascorbic acid (L-AA, vitamin C) to 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), which shows diverse applications in food, cosmetic and pharmaceutical industries. Results In this study,
Yujia Jiang, Jie Zhou, Ruofan Wu, Fengxue Xin, Wenming Zhang, Yan Fang, Jiangfeng Ma, Weiliang Dong, Min Jiang   +8 more
doaj   +2 more sources

Safety evaluation of the food enzyme cyclomaltodextrin glucanotransferase from the non‐genetically modified Anoxybacillus caldiproteolyticus strain AE‐KCGT [PDF]

EFSA Journal
The food enzyme cyclomaltodextrin glucanotransferase ((1–4)‐α‐d‐glucan 4‐α‐d‐[(1–4)‐ α‐d‐glucano]‐transferase; EC 2.4.1.19) is produced with the non‐genetically modified Anoxybacillus caldiproteolyticus strain AE‐KCGT by Amano Enzyme Inc. The food enzyme
EFSA Panel on Food Enzymes (FEZ), Holger Zorn, José Manuel Barat Baviera, Claudia Bolognesi, Francesco Catania, Gabriele Gadermaier, Ralf Greiner, Baltasar Mayo, Alicja Mortensen, Yrjö Henrik Roos, Marize L. M. Solano, Monika Sramkova, Henk Van Loveren, Laurence Vernis, Magdalena Andryszkiewicz, Ana Gomes, Natalia Kovalkovicova, Yi Liu   +17 more
doaj   +2 more sources

The role of arginine 47 in the cyclization and coupling reactions of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 - Implications for product inhibition and product specificity [PDF]

, 2000
Cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) is used for the industrial production of cyclodextrins. Its application, however, is hampered by the limited cyclodextrin product specificity and the strong inhibitory effect of cyclodextrins on ...
Dijkhuizen, L, Dijkstra, B W, Uitdehaag, J C M, van der Veen, Bart A.   +3 more
core   +14 more sources

Did cyclodextrin glycosyltransferases evolve from α‐amylases? [PDF]

FEBS Letters, 1997
The hydrolytic enzymes, α‐amylases, and the cyclodextrin glycosyltransferases (CGTases) are key enzymes in the depolymerization of starch. These two groups of enzymes are evolutionarily related. We propose that the transferase activity is likely to have evolved from an ancestral hydrolase.
del-Rio, Gabriel, Morett, Enrique, Soberon, Xavier   +2 more
openaire   +2 more sources

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

Czech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco, Flávio Faria De Moraes, Natalia Sozza Bernardi, Mary Helen Palmuti Braga Vettori, Rubens Monti, Jonas Contiero   +5 more
doaj   +1 more source

Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]

, 1998
The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra, Bhat, Bradford, Brünger, Davies, Engh, Fujiwara, Haeckel, Harata, Jones, Joost C.M. Uitdehaag, Kabsch, Klein, Klein, Kleywegt, Knegtel, Knegtel, Koshland, Lamzin, Laskowski, Lawson, Lazzaroni, Lubbert Dijkhuizen, Monma, Nakamura, Nakamura, Norman, Pedersen, Penninga, Qian, Read, Reinetta M. Buitelaar, Richèle D. Wind, Sambrook, Sanger, Schmid, Starnes, Strokopytov, Strokopytov, Svensson, Takase, Takusagawa, Tronrud, Wind, Yanisch-Perron   +44 more
core   +2 more sources

Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells [PDF]

, 2017
Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control.
Ant, Thomas H., Charles, Philip D., Dowle, Adam A., Geoghegan, Vincent, Hester, Svenja, Larson, Tony, Rainey, Stephanie M., Sinkins, Steven P., Stainton, Kirsty, Thomas, Benjamin   +9 more
core   +3 more sources

Reassessment of Acarbose as a Transition State Analogue Inhibitor of Cyclodextrin Glycosyltransferase [PDF]

, 1998
The binding of several different active site mutants of Bacillus circulans cyclodextrin glycosyltransferase to the inhibitor acarbose has been investigated through measurement of Ki values.
Dijkstra, Bauke W.,, Mosi, Renee,, Ruiterkamp, Richard,, Sham, Howard,, Uitdehaag, Joost C.M.,, Withers, Stephen G.,   +5 more
core   +2 more sources

Engineering of cyclodextrin glycosyltransferase reaction and product specificity [PDF]

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2000
Many plants produce starch, a high molecular weight polymer of glucose, for storage as a carbon and energy source. These starch molecules are mostly found in seeds (e.g., wheats) or roots (e.g., potato) in the form of granules consisting of two types of glucan polymers: highly branched amylopectin and linear amylose.
, van der Veen BA, J C, Uitdehaag, B W, Dijkstra, L, Dijkhuizen   +3 more
openaire   +4 more sources

Trapping and Characterization of the Reaction Intermediate in Cyclodextrin Glycosyltransferase by Use of Activated Substrates and a Mutant Enzyme [PDF]

, 1997
Cyclodextrin glycosyltransferases (CGTases) catalyze the degradation of starch into linear or cyclic oligosaccharides via a glycosyl transfer reaction occurring with retention of anomeric configuration.
Dijkstra, Bauke W.,, He, Shouming,, Mosi, Renee,, Uitdehaag, Joost,, Withers, Stephen G.,   +4 more
core   +3 more sources