Engineering of factors determining α‐amylase and cyclodextrin glycosyltransferase specificity in the cyclodextrin glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]
European Journal of Biochemistry, 1998 The starch‐degrading enzymes α‐amylase and cyclodextrin glycosyltransferase (CGTase) are functionally and structurally closely related, with CGTases containing two additional domains (called D and E) compared to the three domains of α‐amylases (A, B and C). Amino acid residue 196 (Thermoanaerobacteriumthermosulfurigenes EM1 CGTase numbering) occupies a
Wind, RD, Buitelaar, RM, Dijkhuizen, L
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EFSA Journal, 2023 The food enzyme cyclomaltodextrin glucanotransferase ((1‐4)‐α‐d‐glucan:(1‐4)‐α‐d‐glucan 4‐α‐d‐[(1‐4)‐α‐d‐glucano]‐transferase; EC 2.4.1.19) is produced with the non‐genetically modified bacteria Anoxybacillus caldiproteolyticus strain TCM3‐539 by ...
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) +20 more
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Physico-chemical properties of corn starch modified with cyclodextrin glycosyltransferase [PDF]
, 2016 Cyclodextrin glycosyltransferase (CGTase) has been used to produce cyclodextrins (CDs) from starches, but their ability to modify starches has been barely explored.
Durá, Ángela, Rosell, Cristina M.
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Brazilian Archives of Biology and Technology, 2004 The production of beta- and gamma -CD by CGTase from Bacillus firmus was studied, in regard to the effect of the source and concentration of starch on the yield of CD and CGTase activity.
Ilma Hiroko Higuti +2 more
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Comparison of porous starches obtained from different enzyme types and levels [PDF]
, 2016 The objective was to compare the action of different hydrolases for producing porous corn starches. Amyloglucosidase (AMG), α-amylase (AM), cyclodextrin-glycosyltransferase (CGTase) and branching enzyme (BE) were tested using a range of concentrations ...
Benavent Gil, Yaiza, Rosell, Cristina M.
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Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase
European Journal of Biochemistry, 2002 Cyclodextrin glycosyltransferase (CGTase) uses an α‐retaining double displacement mechanism to catalyze three distinct transglycosylation reactions. To investigate these reactions as catalyzed by the CGTase from Thermoanaerobacterium thermosulfurigenes the enzyme was overproduced (8 mg·L−1 culture) using Bacillus subtilis as a host.
Leemhuis, Hans +2 more
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Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli
BMC Biotechnology, 2018 Background Cyclodextrin glycosyltransferases (CGTases) catalyze the synthesis of cyclodextrins, which are circular α-(1,4)-linked glucans used in many applications in the industries related to food, pharmaceuticals, cosmetics, chemicals, and agriculture,
Lei Wang, Sheng Chen, Jing Wu
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A Cyclodextrin-Based Controlled Release System in the Simulation of In Vitro Small Intestine
Molecules, 2020 A novel cyclodextrin (CD)-based controlled release system was developed in the small intestine to control the rate of drug release, on the premise of enteric-coated tablets.
Danni Zheng +4 more
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Molecular cloning of a cyclodextrin glucanotransferase gene from alkalophilic Bacillus sp. TS1-1 and characterization of the recombinant enzyme [PDF]
, 2006 A cyclodextrin glucanotransferase (CGTase) gene from Bacillus sp. TS1-1 was isolated and cloned into Escherichia coli. Starting from TTG codon, there was an open reading frame composed of 2163 bp (721 amino acids).
Abdul Rashid, Noor Aini +4 more
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Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the γ‐cyclodextrin production [PDF]
European Journal of Biochemistry, 1998 Bacterial cyclodextrin glycosyltransferases use starch to produce cyclic maltooligosaccharides (cyclodextrins) which are of interest in various applications. The cyclization reaction gives rise to a spectrum of ring sizes consisting of predominantly six to eight glucosyl units. Using the enzyme from Bacillus circulans strain no. 8, binding studies have
G, Parsiegla, A K, Schmidt, G E, Schulz
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