Genetic obesity increases pancreatic expression of mitochondrial proteins which regulate cholesterol efflux in BRIN-BD11 insulinoma cells [PDF]
, 2019 Pancreatic β-cells are sensitive to fluctuations in cholesterol content, which can damage the insulin secretion pathway, contributing to the aetiology of type 2 diabetes mellitus.
Caridis, Anna Maria +4 more
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Localization of sterols and oxysterols in mouse brain reveals distinct spatial cholesterol metabolism [PDF]
, 2020 Dysregulated cholesterol metabolism is implicated in a number of neurological disorders. Many sterols, including cholesterol and its precursors and metabolites, are biologically active and important for proper brain function. However, spatial cholesterol
Angelini, Roberto +10 more
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Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux [PDF]
Journal of Biological Chemistry, 2003 Cholesterol efflux from CHOP cells transfected with sterol 27-hydroxylase (CYP27A1) was compared with non-transfected and mock-transfected cells. Transfection caused expression of CYP27A1, formation of 27-hydroxycholesterol, and inhibition of cholesterol biosynthesis. Transfection enhanced cholesterol efflux to apolipoprotein A-I or human plasma by 2-3-
Genevieve, Escher +3 more
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Impaired release of Vitamin D in dysfunctional adipose tissue: New cues on Vitamin D supplementation in obesity [PDF]
, 2017 Context: Vitamin D accumulates in adipose tissue (AT) and vitamin D deficiency is frequent in obesity. Objective: We hypothesize that trafficking of vitamin D is altered in dysfunctional AT.
Azzena, Bruno +9 more
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CYP27A1 inhibits bladder cancer cells proliferation by regulating cholesterol homeostasis [PDF]
Cell Cycle, 2018 CYP27A1, an enzyme involved in regulating cellular cholesterol homeostasis, converts cholesterol into 27-hydroxycholesterol (27-HC). The relationship between CYP27A1 and cell proliferation was studied to determine the role of CYP27A1 in bladder cancer. The expression of CYP27A1 in three bladder cancer cell lines (T24, UM-UC-3 and 5637) were assessed by
Zhijuan Liang +10 more
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TSPO ligands promote cholesterol efflux and suppress oxidative stress and inflammation in choroidal endothelial cells [PDF]
, 2018 Choroidal endothelial cells supply oxygen and nutrients to retinal pigment epithelial (RPE) cells and photoreceptors, recycle metabolites, and dispose of metabolic waste through the choroidal blood circulation.
Bartholomew, Christopher +4 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2021 目的 研究CYP27A1对膀胱癌细胞的影响及其作用机制。方法 qRY-PCR检测膀胱癌细胞中AR和CYP27A1的mRNA水平;采用转染的实验技术使T24细胞稳定表达CYP27A1;MTT实验检测CYP27A1和27-HC对膀胱癌细胞增殖的影响;裸鼠移植瘤模型检测CYP27A1对膀胱癌细胞增殖的影响;ELIAE检测细胞上清液中27-HC的含量。结果 T24细胞中AR处于高表达水平CYP27A1处于低表达水平,5637细胞中AR处于低表达水平,CYP27A1处于高表达水平 ...
景中民, 朱海松, 徐海亮, 张超
doaj
Enzyme activity assay for cholesterol 27-hydroxylase in mitochondria
Journal of Lipid Research, 2006 Mitochondrial cholesterol 27-hydroxylase (CYP27A1) plays an important role in the maintenance of intracellular cholesterol homeostasis. Cholesterol delivery to the mitochondrial inner membrane is believed to be a rate-limiting step for the “acidic ...
Xiaobo Li +3 more
doaj +1 more source
Expression and localization of sterol 27-hydroxylase (CYP27A1) in monkey retina [PDF]
Experimental Eye Research, 2006 Sterol 27-hydroxylase (CYP27A1) is a mitochondrial P-450 enzyme with broad substrate specificity for C27 sterols including 7-ketocholesterol (7kCh). CYP27A1 is widely expressed in human tissues but has not been previously demonstrated in the retina.
Jung Wha, Lee +4 more
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Hormonal regulation of the human sterol 27-hydroxylase gene CYP27A1 [PDF]
Biochemical Journal, 2003 The mitochondrial sterol 27-hydroxylase (CYP27A1) is a multifunctional cytochrome P450 enzyme that catalyses important hydroxylations in the biosynthesis of bile acids and bioactivation of vitamin D3. Previous results [Babiker, Andersson, Lund, Xiu, Deeb, Reshef, Leitersdorf, Diczfalusy and Björkhem (1997) J. Biol. Chem.
Zufan, Araya +2 more
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