Results 31 to 40 of about 589,705 (297)

Reconstruction of cysteine biosynthesis using engineered cysteine-free enzymes [PDF]

Scientific Reports, 2018
AbstractAmino acid biosynthesis pathways observed in nature typically require enzymes that are made with the amino acids they produce. For example, Escherichia coli produces cysteine from serine via two enzymes that contain cysteine: serine acetyltransferase (CysE) and O-acetylserine sulfhydrylase (CysK/CysM).
Fujishima, Kosuke, Wang, Kendrick M., Palmer, Jesse A., Abe, Nozomi, Nakahigashi, Kenji, Endy, Drew, Rothschild, Lynn J.   +6 more
openaire   +2 more sources

NLRP3 Sensing of Diverse Inflammatory Stimuli Requires Distinct Structural Features

Frontiers in Immunology, 2020
The NLRP3 inflammasome is central to host defense and implicated in various inflammatory diseases and conditions. While the favored paradigm of NLRP3 inflammasome activation stipulates a unifying signal intermediate that de-represses NLRP3, this view has
Tabassum Rahman, Abhinit Nagar, Ellen B. Duffy, Kendi Okuda, Neal Silverman, Jonathan A. Harton   +5 more
doaj   +1 more source

Fragmentation of protonated ions of peptides containing cysteine, cysteine sulfinic acid, and cysteine sulfonic acid [PDF]

Journal of the American Society for Mass Spectrometry, 2004
The oxidation of the sulfhydryl group in cysteine to sulfenic acid, sulfinic acid, and sulfonic acid in proteins is important in a number of enzymatic processes. In this study we examined the fragmentation of four peptides containing cysteine, cysteine sulfinic acid (Cys-SO(2)H), and cysteine sulfonic acid (Cys-SO(3)H) in an ion-trap mass spectrometer.
Wang, Yinsheng, Vivekananda, Shetty, Men, Lijie, Zhang, Qibin   +3 more
openaire   +2 more sources

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

Beilstein Journal of Nanotechnology, 2019
This study was designed to evaluate the nano–bio interactions between endogenous biothiols (cysteine and glutathione) with biomedically relevant, metallic nanoparticles (silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs)), in order to assess the
Barbara Pem, Igor M. Pongrac, Lea Ulm, Ivan Pavičić, Valerije Vrček, Darija Domazet Jurašin, Marija Ljubojević, Adela Krivohlavek, Ivana Vinković Vrček   +8 more
doaj   +1 more source

Synergistic Regulation of Nitrogen and Sulfur on Redox Balance of Maize Leaves and Amino Acids Balance of Grains

Frontiers in Plant Science, 2020
As a primary food crop, maize is widely grown around the world. However, the deficiency of essential amino acids, such as lysine, tryptophan, and methionine, results in poor nutritional quality of maize.
Shuoran Liu, Shuai Cui, Xue Zhang, Yin Wang, Guohua Mi, Qiang Gao   +5 more
doaj   +1 more source

The Anti-Fungal Activity of Nitropropenyl Benzodioxole (NPBD), a Redox-Thiol Oxidant and Tyrosine Phosphatase Inhibitor

Antibiotics, 2022
Phylogenetically diverse fungal species are an increasing cause of severe disease and mortality. Identification of new targets and development of new fungicidal drugs are required to augment the effectiveness of current chemotherapy and counter ...
Gina Nicoletti, Kylie White
doaj   +1 more source

Refining the structure−activity relationships of 2-phenylcyclopropane carboxylic acids as inhibitors of O-acetylserine sulfhydrylase isoforms

Journal of Enzyme Inhibition and Medicinal Chemistry, 2019
The lack of efficacy of current antibacterials to treat multidrug resistant bacteria poses a life-threatening alarm. In order to develop enhancers of the antibacterial activity, we carried out a medicinal chemistry campaign aiming to develop inhibitors ...
Joana Magalhães, Nina Franko, Giannamaria Annunziato, Marco Pieroni, Roberto Benoni, Anna Nikitjuka, Andrea Mozzarelli, Stefano Bettati, Anna Karawajczyk, Aigars Jirgensons, Barbara Campanini, Gabriele Costantino   +11 more
doaj   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Thiol Reductases in Deinococcus Bacteria and Roles in Stress Tolerance

Antioxidants, 2022
Deinococcus species possess remarkable tolerance to extreme environmental conditions that generate oxidative damage to macromolecules. Among enzymes fulfilling key functions in metabolism regulation and stress responses, thiol reductases (TRs) harbour ...
Arjan de Groot, Laurence Blanchard, Nicolas Rouhier, Pascal Rey   +3 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source