Results 91 to 100 of about 28,630 (299)

Purification, refolding and autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica

, 2009
The cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence. To further characterize this cysteine proteinase, the recombinant full-length EhCP112 enzyme was expressed and ...
Quintas-Granados, Laura I., Orozco, Esther, Brieba, Luis G., Arroyo, Rossana, Ortega-Lopez, Jaime   +4 more
core   +1 more source

Ecological Adaptation Mechanisms Underlying Successful Plant Reproduction

Advanced Science, EarlyView.
During floral induction, various environmental and endogenous signals converge to regulate the florigen protein, which is transported from leaves to the SAM to initiate flowering. Within the SAM, a complex network of receptor kinases and small peptides orchestrates floral development with high spatiotemporal precision.
Hang Zhao, Shenghui Huo, Yue Xing, Shuxin Zhang, Guilin Li, Yihui Liu, Chongyang Jin, Fuguang Li, Xiaoyang Ge   +8 more
wiley   +1 more source

Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis

Advanced Science, EarlyView.
In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley   +1 more source

Evaluation of the Relationship between Cystatin C Level in Whole Saliva and Chronic Periodontitis [PDF]

Journal of Mashhad Dental School
Introduction: Chronic periodontitis is an infectious disease resulting in inflammation in tooth supporting tissues, advanced attachment loss and bone loss.
Mohammad Hassan Najafi Neshli, Morteza Taheri, Hooshang Rafatpanah, Amir Moeintaghavi, Majid Reza Mokhtari, Maryam Mohammadzade Rezae, Fateme Farazi   +6 more
doaj  

Dynamic Cap‐Mediated Substrate Access and Potent Inhibitor Design of Monkeypox Virus I7L Protease

Advanced Science
Monkeypox virus (MPXV), an orthopoxvirus that has long been endemic in Africa, has posed a significant global health threat since 2022. The I7L protease, a highly conserved cysteine proteinase essential for orthopoxvirus replication, represents a ...
Haixia Su, Guoqing Wu, Muya Xiong, Yuhang Wang, Junyuan Cao, Mengyuan You, Yingchun Xiang, Tianqing Nie, Minjun Li, Gengfu Xiao, Leike Zhang, Qiang Shao, Yechun Xu   +12 more
doaj   +1 more source

Establishment, optimization and validation of a fluorescence polarization-based high-throughput screening assay targeting cathepsin L inhibitors

SLAS Discovery
Cathepsin L (CTSL), a lysosomal cysteine proteinase, is primarily dedicated to the metabolic turnover of intracellular proteins. It is involved in various physiological processes and contributes to pathological conditions such as viral infection, tumor ...
Wenwen Zhou, Baoqing You, Xiaomeng Zhao, Shuyi Si, Yan Li, Jing Zhang   +5 more
doaj   +1 more source

Nanoscale Spatial Organization of ARC High‐ and Low‐Order Assemblies at Excitatory Synapses

Advanced Science, EarlyView.
ARC (Activity‐Regulated Cytoskeleton‐Associated protein) mediates synaptic plasticity by forming nanoscale assemblies in neurons. Using super‐resolution microscopy and time‐resolved anisotropy with targeted tagging, the study reveals low‐order ARC assemblies at synapses colocalizing with AMPARs, semi‐circular structures at endocytic zones, and 60–80 nm
Martina Damenti, Giovanna Coceano, Mariline Mendes Silva, Jonatan Alvelid, Chiara Sgattoni, Andrea Volpato, Lea Rems, Luciano A. Masullo, Eduard M. Unterauer, Rafal Kowalewski, Lucie Delemotte, Erdinc Sezgin, Ralf Jungmann, Ilaria Testa   +13 more
wiley   +1 more source

Endogenous resistance to insect pests in alfalfa: engineering for enhanced resistance [PDF]

, 1997
Alfalfa (Medicago sativa) is a valuable forage crop grown throughout the World. While the crop is resistant to attack by many insect pests, it is subject to potentially severe losses through the action of several specific pests, which are adapted to ...
Mazahery-Laghab, Hojjatollah, Mazahery-Laghab, H.   +1 more
core  

Inhibition of cysteine and aspartyl proteinases in the alfalfa weevil midgut with biochemical and plant-derived proteinase inhibitors

, 2000
Proteolytic activities in alfalfa weevil (Hypera postica) larval midguts have been characterized. Effects of pH, thiol activators, low-molecular weight inhibitors, and proteinase inhibitors (PIs) on general substrate hydrolysis by midgut extracts were ...
Wilhite, Stephen E, Joze Brzin, Smigocki, Ann C, Ann C Smigocki, Thomas C Elden, Brzin, Joze, Stephen E Wilhite, Elden, Thomas C   +7 more
core   +1 more source

Natural Variation of NAR5 Determines Nitrogenase Activity and the Yield in Soybean

Advanced Science, EarlyView.
This study identified NAR5, a gene encoding a subtilisin‐like protease, that regulates nitrogenase activity in soybean nodules. Overexpressing NAR5 delayed nodule senescence, enhancing nitrogenase activity, yield, and low‐nitrogen tolerance. The elite haplotype NAR5HapI‐1 linked to superior nitrogenase activity and greater seed weight has been ...
Chao Ma, Hanyu Zhao, Haojie Feng, Xulun Dong, Lin Chen, Mingliang Yang, Runyi Chen, Chengjun Lei, Chunyan Liu, Qingshan Chen, Dawei Xin, Jinhui Wang   +11 more
wiley   +1 more source