Results 61 to 70 of about 41,955 (238)

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Advanced Science, EarlyView.
A species‐optimized CRISPR platform integrates efficient piggyBac delivery, genome‐scale sgRNA libraries, and parallel screening in two independently engineered Bactrocera dorsalis Cas9 cell lines. Cross‐line consensus analysis filters line‐specific effects, enriches candidates with reproducible in vivo phenotypes, and reveals conserved, species ...
Ziniu Li, Jiao Qiao, Qiuyuan Zhang, Peipei Liu, Chenjun Zheng, Qi Zhou, Mengqi Ji, Zhihui Zhu, Xiaoxue Li, Hongyu Zhang   +9 more
wiley   +1 more source

Increased Degradation Rates in the Components of the Mitochondrial Oxidative Phosphorylation Chain in the Cerebellum of Old Mice

Frontiers in Aging Neuroscience, 2018
Brain structures differ in the magnitude of age-related neuron loss with the cerebellum being more affected. An underlying cause could be an age-related decline in mitochondrial bioenergetics. Successful aging of mitochondria reflects a balanced turnover
Aurel Popa-Wagner, Aurel Popa-Wagner, Aurel Popa-Wagner, Raluca E. Sandu, Coman Cristin, Adriana Uzoni, Kevin A. Welle, Jennifer R. Hryhorenko, Sina Ghaemmaghami   +8 more
doaj   +1 more source

Loss of COX4I1 Leads to Combined Respiratory Chain Deficiency and Impaired Mitochondrial Protein Synthesis

Cells, 2021
The oxidative phosphorylation (OXPHOS) system localized in the inner mitochondrial membrane secures production of the majority of ATP in mammalian organisms. Individual OXPHOS complexes form supramolecular assemblies termed supercomplexes.
Kristýna Čunátová, David Pajuelo Reguera, Marek Vrbacký, Erika Fernández-Vizarra, Shujing Ding, Ian M. Fearnley, Massimo Zeviani, Josef Houštěk, Tomáš Mráček, Petr Pecina   +9 more
doaj   +1 more source

In vitro studies on cytochrome c biogenesis

, 2003
C-type cytochromes are essential for almost all organisms and are mainly involved in electron transport; they are characterised by the covalent attachment of heme to protein through two thioether bonds to a CXXCH peptide motif. This thesis describes the development of in vitro systems to establish chemical aspects of the process of cytochrome c ...
Daltrop, O, Daltrop, Oliver
openaire   +2 more sources

Photobiomodulation‑Engineered Extracellular Vesicles Enhance Neural Differentiation via UFL1‑Mediated UFMylation in Spinal Cord Injury

Advanced Science, EarlyView.
Photobiomodulated microglia release engineered extracellular vesicles that deliver UFL1 to the injured spinal cord. UFL1 competitively binds p53 with MDM2, inhibiting p53 ubiquitination and stabilizing p53 signaling. This dual mechanism simultaneously promotes anti‐inflammatory microglial polarization and directs neural stem cell differentiation toward
Yunxiao Fang, Zuomeng Wu, Ruocheng Guo, Cancan Wang, Yue Qin, Yixiang Dong, Aoyun Li, Ao Liu, Chongyu Jia, Tianyu Han, Peiwen Song, Lei Chen, Cailiang Shen   +12 more
wiley   +1 more source

COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis

eLife, 2018
Cytochrome c oxidase of the mitochondrial oxidative phosphorylation system reduces molecular oxygen with redox equivalent-derived electrons. The conserved mitochondrial-encoded COX1- and COX2-subunits are the heme- and copper-center containing core ...
Abhishek Aich, Cong Wang, Arpita Chowdhury, Christin Ronsör, David Pacheu-Grau, Ricarda Richter-Dennerlein, Sven Dennerlein, Peter Rehling   +7 more
doaj   +1 more source

Biogenesis of cbb3-type cytochrome c oxidase in Rhodobacter capsulatus

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012
The cbb(3)-type cytochrome c oxidases (cbb(3)-Cox) constitute the second most abundant cytochrome c oxidase (Cox) group after the mitochondrial-like aa(3)-type Cox. They are present in bacteria only, and are considered to represent a primordial innovation in the domain of Eubacteria due to their phylogenetic distribution and their similarity to nitric ...
Ekici, Seda, Pawlik, Grzegorz, Lohmeyer, Eva, Koch, Hans-Georg, Daldal, Fevzi   +4 more
openaire   +4 more sources

Investigations of the Evolved Molecular Basis for Terpenoid Biosynthesis in Marine Sponges

Advanced Science, EarlyView.
Confirming and extending a previous observation in another Bubarida sponge, genomic and functional analyses of A. cavernosa reveal that sponges retain the mevalonate pathway and employ single α‐domain T1TSs and UbiA‐type TSs for terpenoid biosynthesis. The absence of T1TSs clustering with other biosynthetic genes tentatively suggests, based on limited ...
Fangyan Chen, Bao Chen, Wenhui Zhang, Guifang Sun, Yao Zhao, Jianpeng Zhang, Baofu Xu   +6 more
wiley   +1 more source

Mmicular mechanisms of cytochrome c biogenesis: three distinct systems [PDF]

Molecular Microbiology, 1998
The past 10 years have heralded remarkable progress in the understanding of the biogenesis of c‐type cytochromes. The hallmark of c‐type cytochrome synthesis is the covalent ligation of haem vinyl groups to two cysteinyl residues of the apocytochrome (at a Cys–Xxx–Yyy–Cys–His signature motif).
R, Kranz, R, Lill, B, Goldman, G, Bonnard, S, Merchant   +4 more
openaire   +2 more sources

Enhancing Meat Quality Through Dietary Fiber: Insights Into the Gut Microbiota‐Derived Short‐Chain Fatty Acids‐Muscle Axis

Animal Research and One Health, EarlyView.
Dietary fiber (DF) modulates meat quality through the gut‐muscle axis by reshaping gut microbiota, improving intestinal integrity, reducing inflammation, and regulating muscle metabolism. These mechanisms influence muscle fiber characteristics and post‐mortem biochemistry, thereby enhancing tenderness, juiciness, color, flavor, and nutritional value of
Zeshan Zulfiqar, Muhammad Arslan Asif, Layla Al‐Mitib, Nabeela Ashiq, Haojie Wang, Yalei Cui, Boshuai Liu, Shucheng Huang, Yinghua Shi   +8 more
wiley   +1 more source