Results 141 to 150 of about 25,918 (263)

Cytosine Base Editor-Mediated Multiplex Genome Editing to Accelerate Discovery of Novel Antibiotics in Bacillus subtilis and Paenibacillus polymyxa. [PDF]

Front Microbiol, 2021
Kim MS, Kim HR, Jeong DE, Choi SK.
europepmc   +1 more source

Gene Editing for Haemophilia—The Next Frontier

Haemophilia, EarlyView.
ABSTRACT The recently approved haemophilia A and B gene therapies via adeno‐associated virus (AAV) showed a promising therapeutic response after a single injection, but there are still limitations, including the potential loss of transgene expression and restriction in adults.
Mirko Pinotti, Gregory A. Newby, Sundar Selvaraj, Steven W. Pipe   +3 more
wiley   +1 more source

Cytosolic delivery of bacterial metabolites by riboflavin transporters promotes MR1 antigen presentation and MAIT cell recognition

Immunology &Cell Biology, EarlyView.
VitBAg antigen presentation is promoted through a cytosolic pathway that reaches ligand‐receptive MR1 to present to MAIT cells, like the riboflavin transport pathway. Riboflavin solute carrier transporters can promote this MR1 presentation, but they are not essential for this role, suggesting redundant pathways to uptake VitBAg.
Sebastian Cruz‐Gomez, Hui Jing Lim, Brunda Nijagal, Yuting Yang, Nicole Lau, Jeffrey YW Mak, David P Fairlie, Hayley Newton, Mariolina Salio, Jose A Villadangos, Hamish EG McWilliam   +10 more
wiley   +1 more source

Integrative taxonomy reveals four new species of the armoured catfish genus Pareiorhina (Siluriformes: Loricariidae) from the upper Paraná River basin, Brazil

Journal of Fish Biology, EarlyView.
Abstract Four new species of the small‐sized armoured catfish genus Pareiorhina are described from mountain ranges in the Grande River drainage, upper Paraná River basin, based on morphological and molecular species delimitation methods. Molecular analyses based on the cytochrome oxidase subunit I (COI) marker recovered Pareiorhina as polyphyletic ...
Pedro L. C. Uzeda, Luana J. Sartori, Axel M. Katz, Felipe P. Ottoni, Wilson J. E. M. Costa, Francisco Langeani, Valter M. Azevedo‐Santos   +6 more
wiley   +1 more source

Monitoring of haematopoietic stem cell mobilization by targeted DNA methylation analysis for the British Journal of Haematology

British Journal of Haematology, EarlyView.
Wouter H. G. Hubens, Anke Diehlmann, Patrick Wuchter, Wolfgang Wagner   +3 more
wiley   +1 more source

DNA extracted from boiled archival fish bones yields high‐quality whole‐genome sequencing data

Journal of Fish Biology, EarlyView.
Abstract Archival samples provide a unique source of organismal DNA, offering the potential to extend the temporal scale of genetic studies by decades to centuries. Fish hard structures, such as otoliths and scales, serve as records for fish collected during fisheries monitoring across a large spatiotemporal scale.
Jingyao Niu, Anti Vasemägi, María‐Eugenia López, Lilian Pukk, Magnus Huss, Anna Gårdmark   +5 more
wiley   +1 more source

Efficient Single Base Editing in Mouse Using Cytosine Base Editor 4

, 2019
Most human genetic disease arises from point mutations. These genetic diseases can theoretically be corrected by gene therapy but clinic practice is still far from mature. Nearly half of the pathogenic single-nucleotide polymorphisms (SNPs) are caused by G:C>A:T or T:A>C:G base changes.
Adlat, Salah, Yang, Ping, Chen, Yang, Sah, Rajiv Kumar, Oo, Zin Mar, Myint, May Zun Zaw, Hayel, Farooq, Bahadar, Noor, Al-Azab, Mahmoud, Bah, Fatoumata Binta, Zhang, Luqing, Zheng, Yaowu, Feng, Xuechao   +12 more
openaire  

Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids

Journal of Fish Biology, EarlyView.
Abstract Methods using environmental nucleic acids have become highly effective for monitoring aquatic biodiversity, with an array of suitable use cases, including metrics for fisheries assessment. Traditional methods for assessing fish populations often rely on invasive techniques with limited spatial and temporal coverage.
Ana Ramón‐Laca, Krista M. Nichols, Kim M. Parsons, Ramón Gallego, David R. Vieites, Ignacio Doadrio, Ana Riesgo   +6 more
wiley   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

An innovative approach using CRISPR-ribonucleoprotein packaged in virus-like particles to generate genetically engineered mouse models

Nature Communications
Genetically engineered mouse models (GEMMs) are crucial for investigating disease mechanisms, developing therapeutic strategies, and advancing fundamental biological research. While CRISPR gene editing has greatly facilitated the creation of these models,
Tae Yeong Jeong, Da Eun Yoon, Sol Pin Kim, Jiyun Yang, Soo-Yeon Lim, Sungjin Ok, Sungjin Ju, Jeongeun Park, Su Bin Lee, Soo-Ji Park, Sanghun Kim, Hyunji Lee, Daekee Lee, Soo Kyung Kang, Seung Eun Lee, Hyeon Soo Kim, Je Kyung Seong, Kyoungmi Kim   +17 more
doaj   +1 more source