Results 151 to 160 of about 15,403 (255)

High-efficiency TadA cytosine base editors for precise modelling of human disease variants

Nature Biomedical Engineering
Many missense mutations identified in genetic testing are variants of uncertain significance (VUS), not yet classified as either benign or pathogenic. Systematic determination of their functional relevance is a pressing clinical need. CRISPR-mediated base editing can precisely introduce precise variants into model organisms for functional testing, but ...
Wei Qin, Sheng-Jia Lin, Yu Zhang, Kevin Huang, Cassidy Petree, Pratishtha Varshney, Gaurav K. Varshney   +6 more
openaire   +2 more sources

Transcriptomic and proteomic signatures underlying nymphal adaptation and foam production in the forage pest Mahanarva spectabilis

Insect Molecular Biology, EarlyView.
RNA‐seq and Batelli gland proteomics of fifth‐instar Mahanarva spectabilis nymphs reveal transcripts and proteins associated with xylem feeding, foam production and environmental interaction. Functional annotation identified genes involved in osmoregulation, detoxification, chemosensation and stress responses, while proteomic analysis confirmed ...
Monique da Silva Bonjour, Angelo José Rinaldi, Eulálio Gutemberg Dias dos Santos, Gabriely Teixeira Bhering Faria, Ana Márcia Escocard de Azevedo Manhães, Jorge Fernando Pereira, Alexander Machado Auad, Maria Goreti de Almeida Oliveira, Humberto Josué de Oliveira Ramos   +8 more
wiley   +1 more source

High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase

Nature Biotechnology
Base editors (BEs), covalent fusions of a cytosine or adenine deaminase with a nuclease-impaired CRISPR protein, mediate site-specific conversion of C:G to T:A (CBEs) or A:T to G:C (ABEs) in the genome. Existing BEs modify all cytosines or adenines within the editing window, which limits their precision.
Yuan Wu, Yu-Lan Xiao, Weixin Tang
openaire   +2 more sources

Genome assembly of Elcysma westwoodi provides insights into host‐plant cyanogenic glucoside detoxification and chemical defence gene evolution

Insect Molecular Biology, EarlyView.
A chromosome‐level genome of Elcysma westwoodi was assembled. The genome encodes 272 detoxification‐related genes. A single‐copy bCAS was retained for cyanide detoxification. CYP405A and CYP332A were recruited into cyanogenic defence evolution. UGT33A retained a conserved GT‐B fold and catalytic domains.
Minyoung Choi, Murtaza Khan, Juil Kim
wiley   +1 more source

Base Editing: Expanding the Types of DNA Damage Products Harnessed for Genome Editing [PDF]

Base editors are an innovative addition to the genome editing toolbox that introduced a new genome editing strategy to the field. Instead of using double-stranded DNA breaks, base editors utilize unique types of DNA damage products featuring nucleobase ...
Gu, Sifeng
core  

TadA-Derived Cytosine Base Editor for Precise Genome Editing in Zebrafish

Abstract CRISPR base editors are crucial for precise genome manipulation. Existing APOBEC-based cytosine base editors (CBEs), while powerful, exhibit indels and sequence context limitations, where TC-context preferences restrict effective editing of CC and GC motifs.
Wei Qin, Sheng-Jia Lin, Cassidy Petree, Pratishtha Varshney, Gaurav K. Varshney   +4 more
openaire   +1 more source

Integrative taxonomy reveals four new species of the armoured catfish genus Pareiorhina (Siluriformes: Loricariidae) from the upper Paraná River basin, Brazil

Journal of Fish Biology, EarlyView.
Abstract Four new species of the small‐sized armoured catfish genus Pareiorhina are described from mountain ranges in the Grande River drainage, upper Paraná River basin, based on morphological and molecular species delimitation methods. Molecular analyses based on the cytochrome oxidase subunit I (COI) marker recovered Pareiorhina as polyphyletic ...
Pedro L. C. Uzeda, Luana J. Sartori, Axel M. Katz, Felipe P. Ottoni, Wilson J. E. M. Costa, Francisco Langeani, Valter M. Azevedo‐Santos   +6 more
wiley   +1 more source

DNA extracted from boiled archival fish bones yields high‐quality whole‐genome sequencing data

Journal of Fish Biology, EarlyView.
Abstract Archival samples provide a unique source of organismal DNA, offering the potential to extend the temporal scale of genetic studies by decades to centuries. Fish hard structures, such as otoliths and scales, serve as records for fish collected during fisheries monitoring across a large spatiotemporal scale.
Jingyao Niu, Anti Vasemägi, María‐Eugenia López, Lilian Pukk, Magnus Huss, Anna Gårdmark   +5 more
wiley   +1 more source

Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids

Journal of Fish Biology, EarlyView.
Abstract Methods using environmental nucleic acids have become highly effective for monitoring aquatic biodiversity, with an array of suitable use cases, including metrics for fisheries assessment. Traditional methods for assessing fish populations often rely on invasive techniques with limited spatial and temporal coverage.
Ana Ramón‐Laca, Krista M. Nichols, Kim M. Parsons, Ramón Gallego, David R. Vieites, Ignacio Doadrio, Ana Riesgo   +6 more
wiley   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source