Results 151 to 160 of about 25,918 (263)

Comprehensive evaluation and prediction of editing outcomes for near-PAMless adenine and cytosine base editors

Communications Biology
Summary Base editors enable the direct conversion of target bases without inducing double-strand breaks, showing great potential for disease modeling and gene therapy. Yet, their applicability has been constrained by the necessity for specific protospacer adjacent motif (PAM).
Xiaoyu Zhou, Jingjing Gao, Liheng Luo, Changcai Huang, Jiayu Wu, Xiaoyue Wang   +5 more
openaire   +3 more sources

Optimizations of Cas12a‐ and Cas12i‐based adenine base editors for efficient precision editing in the plant genome

Journal of Integrative Plant Biology, EarlyView.
A strategy coupling high‐activity nucleases with dimeric TadA‐8e optimizes plant Cas12‐based adenine base editors, boosts editing efficiency, and provides precise editors for crop breeding and genomics research.
Xiaoshuang Liu, Shan Jin, Zhi Xiao, Chong Ma, Qing Wang, Huanhuan Wang, Ruohan Zhou, Dongfang Gu, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei   +11 more
wiley   +1 more source

High-efficiency TadA cytosine base editors for precise modelling of human disease variants

Nature Biomedical Engineering
Many missense mutations identified in genetic testing are variants of uncertain significance (VUS), not yet classified as either benign or pathogenic. Systematic determination of their functional relevance is a pressing clinical need. CRISPR-mediated base editing can precisely introduce precise variants into model organisms for functional testing, but ...
Wei Qin, Sheng-Jia Lin, Yu Zhang, Kevin Huang, Cassidy Petree, Pratishtha Varshney, Gaurav K. Varshney   +6 more
openaire   +2 more sources

Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications

Journal of Integrative Plant Biology, EarlyView.
Pentatricopeptide repeat proteins coordinate nucleus‐organelle communication by modulating RNA metabolism within chloroplasts and mitochondria. This review highlights how they control critical processes like photosynthesis, seed development, fertility restoration, and stress survival in crops, and explores their potential as programmable tools for RNA ...
Mingming Wu, Maohong Cai, Rongrong Zhai, Jing Ye, Guofu Zhu, Faming Yu, Shenghai Ye, Xiaoming Zhang   +7 more
wiley   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase

Nature Biotechnology
Base editors (BEs), covalent fusions of a cytosine or adenine deaminase with a nuclease-impaired CRISPR protein, mediate site-specific conversion of C:G to T:A (CBEs) or A:T to G:C (ABEs) in the genome. Existing BEs modify all cytosines or adenines within the editing window, which limits their precision.
Yuan Wu, Yu-Lan Xiao, Weixin Tang
openaire   +2 more sources

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source

TadA-Derived Cytosine Base Editor for Precise Genome Editing in Zebrafish

Abstract CRISPR base editors are crucial for precise genome manipulation. Existing APOBEC-based cytosine base editors (CBEs), while powerful, exhibit indels and sequence context limitations, where TC-context preferences restrict effective editing of CC and GC motifs.
Wei Qin, Sheng-Jia Lin, Cassidy Petree, Pratishtha Varshney, Gaurav K. Varshney   +4 more
openaire   +1 more source

DNA barcoding and geometric morphometry of tabanid flies (Diptera: Tabanidae) in Thailand and a new record of a Thai horse fly

Medical and Veterinary Entomology, EarlyView.
Tabanus tenens identified in this study is a newly recorded species in Thailand. DNA barcoding using the cox1 gene identified tabanid species in the present study. WGM data from different sites served as a reference for species identification. Abstract Tabanid flies are gaining high medical and veterinary importance due to their role as a vector of ...
Nantatchaporn Klaiklueng, Weluga Bootsongkorn, Patsharaporn T. Sarasombath, Pichet Ruenchit, Sirichit Wongkamchai   +4 more
wiley   +1 more source

Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides

Plant Biotechnology Journal, EarlyView.
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew, Aron Ferenczi, Marie Dannay, Cristina Ponce‐Lilly, Adam Kovac, Dávid Tóth, Szilvia Z. Tóth, Attila Molnar   +7 more
wiley   +1 more source