Results 161 to 170 of about 15,403 (255)

Optimizations of Cas12a‐ and Cas12i‐based adenine base editors for efficient precision editing in the plant genome

Journal of Integrative Plant Biology, EarlyView.
A strategy coupling high‐activity nucleases with dimeric TadA‐8e optimizes plant Cas12‐based adenine base editors, boosts editing efficiency, and provides precise editors for crop breeding and genomics research.
Xiaoshuang Liu, Shan Jin, Zhi Xiao, Chong Ma, Qing Wang, Huanhuan Wang, Ruohan Zhou, Dongfang Gu, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei   +11 more
wiley   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source

Natural variation in the promoter of OsPDX1.2 enhances vitamin B6 accumulation and cold tolerance in rice

Journal of Integrative Plant Biology, EarlyView.
Allelic variation in the promoter of the pyridoxal phosphate synthase OsPDX1.2 caused variation in the ACE‐box cis‐elements, resulting in differing OsPDX1.2 expression due to differences in binding affinity between the OsbZIP18 transcriptipn factor and the ACE‐box, contributing to variation in vitamin B6 levels and cold tolerance among rice varieties ...
Bi Wang, Qiu Yi Wang, Wen Jie Shi, Rong Hui Li, Xu Dong Zhang, Li Ping Wang, Shi Jia Xia, Kai Xi Chen, Jun Jie Zhou, Yang Yang Sun, Jing He, Alisdair R. Fernie, Ying Chun Han, Jun Zhou Li, Yu Heng Shi, Xian Qing Liu, Yuan Yuan Zhang, Jie Luo, Cheng Jin   +18 more
wiley   +1 more source

Developing next-generation tools for metabolic engineering in non-conventional yeast Yarrowia lipolytica

Blenner, MarkMetabolic engineering is a promising alternative to producing chemicals over chemical synthesis and plant extraction. Metabolic engineering primarily involves changing the flux to produce target molecules by changing the genetic information.
Ganesan, Vijaydev
core   +1 more source

Complete organellar genomes of Cystophora brownii: Insights into mitochondrial and plastid structure and evolution in brown algae

Journal of Phycology, EarlyView.
Abstract Cystophora (order Fucales) is one of the most diverse genera of habitat‐forming brown algae and is endemic to temperate reefs of Australia and New Zealand. Species in this genus form complex habitat structures that support diverse marine communities and contribute to coastal productivity. However, Cystophora spp.
Jane M. Edgeloe, Samuel Starko, Georgina V. Wood, Albert Pessarrodona, Antoine J. P. Minne, Thomas Wernberg   +5 more
wiley   +1 more source

Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg. [PDF]

J Biol Eng
Shon YJ, Baek D, Jin SB, Kim WJ, Jung GY, Lim HG.   +5 more
europepmc   +1 more source

Correction of pathogenic mitochondrial DNA in patient-derived disease models using mitochondrial base editors

Contains fulltext : 320593.pdf (Publisher’s version ) (Open Access)Mutations in the mitochondrial genome can cause maternally inherited diseases, cancer, and aging-related conditions.
Joore, I.P., Vermeij, W.P., Rodenburg, R.J.T., Jiménez-Curiel, E., Nagyova, E., Nieuwenhuis, E.E.S., Koppens, M.A.J., Shehata, S., van Hasselt, P.M., Fuchs, S.A., Smit, K, Castro-Alpízar, J., Levy, N., Schiffelers, R., Tang, Z., Muffels, I.   +15 more
core   +1 more source

A Single‐Nucleotide Substitution Generates a de Novo Promoter That Activates a Latent Metabolic Bypass in Escherichia coli

Molecular Microbiology, EarlyView.
A chromosomal mutation allows bypass of deletion of the Escherichia coli fabH gene. The bypass results from creation of a new promoter that drives increased madA transcription, resulting in increased synthesis of acetyl‐acyl carrier protein by decarboxylation of malonyl‐acyl carrier protein.
Qiaoqiao Guo, Qi Zou, Jilong Qin, Makrina Totsika, Yaoqin Hong, John E. Cronan   +5 more
wiley   +1 more source

DNA barcoding and geometric morphometry of tabanid flies (Diptera: Tabanidae) in Thailand and a new record of a Thai horse fly

Medical and Veterinary Entomology, EarlyView.
Tabanus tenens identified in this study is a newly recorded species in Thailand. DNA barcoding using the cox1 gene identified tabanid species in the present study. WGM data from different sites served as a reference for species identification. Abstract Tabanid flies are gaining high medical and veterinary importance due to their role as a vector of ...
Nantatchaporn Klaiklueng, Weluga Bootsongkorn, Patsharaporn T. Sarasombath, Pichet Ruenchit, Sirichit Wongkamchai   +4 more
wiley   +1 more source