Results 141 to 150 of about 1,101 (168)

Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose

Food Chemistry, 2023
D-Allulose, as low-calorie rare sugar, possessed several notable biological activities and was biosynthesized by D-allulose 3-epimerase (DAEase). Here, CcDAE from Clostridium cellulolyticum was successfully immobilization via covalent attachment (RI-CcDAE), and Resin-SpyCatcher/SpyTag-CcDAE modular (DI-CcDAE).
Xin, Gao, Cancan, Wei, Hongbin, Qi, Chao, Li, Fuping, Lu, Hui-Min, Qin   +5 more
openaire   +2 more sources

D-allulose 3-epimerase for low-calorie D-allulose synthesis: microbial production, characterization, and applications

Critical Reviews in Biotechnology
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization ...
Xiaofang Xie, Caiming Li, Xiaofeng Ban, Hongshun Yang, Zhaofeng Li   +4 more
openaire   +2 more sources

d-Allulose enhances postprandial fat oxidation in healthy humans

Nutrition, 2017
d-Allulose, a C-3 epimer of d-fructose, has been reported to decrease body weight and adipose tissue weight in animal studies and is expected to be a potent antiobese sweetener. Our animal study suggested that one of the mechanisms of d-allulose's antiobesity function is an increase in energy expenditure.
Tomonori, Kimura, Akane, Kanasaki, Noriko, Hayashi, Takako, Yamada, Tetsuo, Iida, Yasuo, Nagata, Kazuhiro, Okuma   +6 more
openaire   +2 more sources

D-allulose, a versatile rare sugar: recent biotechnological advances and challenges

Critical Reviews in Food Science and Nutrition, 2021
D-Allulose is the C-3 epimer of D-fructose, and widely regarded as a promising substitute for sucrose. It's an excellent low-calorie sweetener, with 70% sweetness of sucrose, 0.4 kcal/g dietary energy, and special physiological functions. It has been approved as GRAS by the U.S. Food and Drug Administration, and is allowed to be excluded from total and
Wenli Zhang, Ding Chen, Jiajun Chen, Wei Xu, Qiuming Chen, Hao Wu, Cuie Guang, Wanmeng Mu   +7 more
openaire   +2 more sources

Insulin mimetic effect of D‐allulose on apolipoprotein A‐I gene

Journal of Food Biochemistry, 2022
Several nutrients modulate the transcriptional activity of the apolipoprotein A-I (apo A-I) gene. To determine the influence of rare sugars on apo A-I expression in hepatic (HepG2) and intestinal derived (Caco-2) cell lines, apo A-I, albumin, and SP1 were quantified with enzyme immunoassay and Western blots while mRNA levels were quantified with real ...
Michael J. Haas, Shrina Parekh, Poonam Kalidas, Angela Richter, Firas Warda, Norman C. W. Wong, Masaaki Tokuda, Arshag D. Mooradian   +7 more
openaire   +2 more sources

Bioproduction of D‐allulose: Properties , applications, purification, and future perspectives

Comprehensive Reviews in Food Science and Food Safety, 2021
Abstract D‐allulose is the C‐3 epimer of D‐fructose, which rarely exists in nature, and can be biosynthesized from D‐fructose by the catalysis of D‐psicose 3‐epimerase. D‐allulose is safe for human consumption and was recently approved by the United States Food and Drug Administration for food applications. It is not only able be used
Mengying Hu, Mengli Li, Bo Jiang, Tao Zhang   +3 more
openaire   +2 more sources

Tailored magnetic silica-immobilized D-allulose 3-epimerase with enhanced stability and recyclability for efficient D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie functional sweetener, is produced by the enzymatic conversion of d-fructose via D-allulose 3-epimerase (DAE) and holds significant market potential, particularly for individuals with obesity and diabetes. However, the limited reusability and stability of DAE have restricted its industrial application.
Ziqun, Xiao, Bo, Jiang, Longbei, Xiang, Ran, Zhang, Jingjing, Chen   +4 more
openaire   +2 more sources

Advances in the biosynthesis of D-allulose

World Journal of Microbiology and Biotechnology
D-allulose is a rare monosaccharide and a C-3 epimer of D-fructose. It has physiological functions, such as antihyperglycemic, obesity-preventing, neuroprotective, and reactive oxygen species (ROS) scavenging effects, making it an ideal sugar substitute. The synthesis methods for D-allulose include chemical synthesis and biosynthesis.
Yue, Zhang, Zhengsong, Zhou, Haoni, Luan, Xue, Zhang, Mengyu, Liu, Kuiming, Wang, Fei, Wang, Wei, Feng, Wei, Xu, Peng, Song   +9 more
openaire   +2 more sources

A study of D-allulose-associated reproductive toxicity in rats

Food and Chemical Toxicology, 2019
In this study, we assessed whether D-allulose was associated with reproductive toxicity in rats, assessing reproduction and offspring growth following gavage of parents with 0, 500, 1000, and 2000 mg/kg of this compound. Specifically, female rats were continuously dose from 2 weeks prior to mating until day 21 of lactation, while males were dose for ...
Hyejung, Kim, Chongjin, Park, Soonok, Sa, Iris, Case, Chunmei, Li, Yonglin, Gao, Hongbo, Wang, Jingwei, Tian   +7 more
openaire   +2 more sources

Engineering Escherichia coli for D-allulose biosynthesis from glycerol

Journal of Biotechnology
D-allulose, a naturally occurring monosaccharide, is present in small quantities in nature. It is considered a valuable low-calorie sweetener due to its low absorption in the digestive tract and zero energy for growth. Most of the recent efforts to produce D-allulose have focused on in vitro enzyme catalysis. However, microbial fermentation is emerging
Qiang, Guo, Zhen-Xing, Dong, Xuan, Luo, Ling-Jie, Zheng, Li-Hai, Fan, Hui-Dong, Zheng   +5 more
openaire   +2 more sources