Results 151 to 160 of about 1,145 (182)

Tailored magnetic silica-immobilized D-allulose 3-epimerase with enhanced stability and recyclability for efficient D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie functional sweetener, is produced by the enzymatic conversion of d-fructose via D-allulose 3-epimerase (DAE) and holds significant market potential, particularly for individuals with obesity and diabetes. However, the limited reusability and stability of DAE have restricted its industrial application.
Ziqun, Xiao, Bo, Jiang, Longbei, Xiang, Ran, Zhang, Jingjing, Chen   +4 more
openaire   +2 more sources

Advances in the biosynthesis of D-allulose

World Journal of Microbiology and Biotechnology
D-allulose is a rare monosaccharide and a C-3 epimer of D-fructose. It has physiological functions, such as antihyperglycemic, obesity-preventing, neuroprotective, and reactive oxygen species (ROS) scavenging effects, making it an ideal sugar substitute. The synthesis methods for D-allulose include chemical synthesis and biosynthesis.
Yue, Zhang, Zhengsong, Zhou, Haoni, Luan, Xue, Zhang, Mengyu, Liu, Kuiming, Wang, Fei, Wang, Wei, Feng, Wei, Xu, Peng, Song   +9 more
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A study of D-allulose-associated reproductive toxicity in rats

Food and Chemical Toxicology, 2019
In this study, we assessed whether D-allulose was associated with reproductive toxicity in rats, assessing reproduction and offspring growth following gavage of parents with 0, 500, 1000, and 2000 mg/kg of this compound. Specifically, female rats were continuously dose from 2 weeks prior to mating until day 21 of lactation, while males were dose for ...
Hyejung, Kim, Chongjin, Park, Soonok, Sa, Iris, Case, Chunmei, Li, Yonglin, Gao, Hongbo, Wang, Jingwei, Tian   +7 more
openaire   +2 more sources

Engineering Escherichia coli for D-allulose biosynthesis from glycerol

Journal of Biotechnology
D-allulose, a naturally occurring monosaccharide, is present in small quantities in nature. It is considered a valuable low-calorie sweetener due to its low absorption in the digestive tract and zero energy for growth. Most of the recent efforts to produce D-allulose have focused on in vitro enzyme catalysis. However, microbial fermentation is emerging
Qiang, Guo, Zhen-Xing, Dong, Xuan, Luo, Ling-Jie, Zheng, Li-Hai, Fan, Hui-Dong, Zheng   +5 more
openaire   +2 more sources

Designable immobilization of D-allulose 3-epimerase on bimetallic organic frameworks based on metal ion compatibility for enhanced D-allulose production

International Journal of Biological Macromolecules
D-allulose, a low-calorie rare sugar catalyzed by D-allulose 3-epimerase (DAE), is highly sought after for its potential health benefits. However, poor reusability and stability of DAE limited its popularization in industrial applications. Although metal-organic frameworks (MOFs) offer a promising enzyme platform for enzyme immobilization, developing ...
Huayang, Tang, Yian, Chen, Dexun, Fan, Fengguang, Zhao, Shuangyan, Han   +4 more
openaire   +2 more sources

Inhibition of 3T3-L1 Adipocyte Differentiation by D-allulose

Biotechnology and Bioprocess Engineering, 2020
Obesity is a serious problem in modern society and its prevalence continues to increase worldwide, resulting in metabolic disorder related diseases. D-allulose, a sugar substitute, boasts a near-zero calorie value and regulates lipid accumulation. However, the molecular mechanism of D-allulose at the cellular level has not been fully elucidated.
Seohyun Moon, Yang Hee Kim, Kyungoh Choi
openaire   +1 more source

Elucidation of d-allulose recognition mechanism in ketose 3-epimerase

Journal of Bioscience and Bioengineering
d-Allulose is a low-calorie sweetener with multiple nutritional functions that can be produced through d-fructose isomerization by ketose 3-epimerase (KEase). l-Ribulose 3-epimerase from Arthrobacterglobiformis (AgLRE) is one of the most important enzymes that produce d-allulose; however, its substrate recognition mechanism is unknown.
Masahiro Watanabe, Yusuke Nakamichi, Shohei Mine   +2 more
openaire   +2 more sources

Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor

Biotechnology Journal
AbstractBackgroundD‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase).
Zijie Li, Yangfan Hu, Cheng Yu, Kangqing Fei, Liqun Shen, Yishi Liu, Hideki Nakanishi   +6 more
openaire   +2 more sources

Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Bioprocess and Biosystems Engineering
D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-DAE (Tm = 98.4 ± 0.7 ℃) from Thermotoga sp. was identified via in silico screening.
Ji-Dong, Shen, Bao-Ping, Xu, Te-Li, Yu, Yong-Xiang, Fei, Xue, Cai, Liang-Gang, Huang, Li-Qun, Jin, Zhi-Qiang, Liu, Yu-Guo, Zheng   +8 more
openaire   +2 more sources

One-pot production of d-allulose from inulin by a novel identified thermostable exoinulinase from Aspergillus piperis and Dorea sp. d-allulose 3-epimerase

Process Biochemistry, 2020
Abstract Inulin has been widely used as a cheap bioresource for producing many valuable products by enzymatic hydrolysis or microbial fermentation, such as high-fructose syrup and fructooligosaccharides. In this work, a one-pot two-enzyme reaction system was developed to produce d -allulose from inulin using A. piperis exoinulinase and Dorea sp. d -
Wen Li, Yingying Zhu, Xiangling Jiang, Wenli Zhang, Cuie Guang, Wanmeng Mu   +5 more
openaire   +1 more source