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Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Bioprocess and Biosystems Engineering
D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-DAE (Tm = 98.4 ± 0.7 ℃) from Thermotoga sp. was identified via in silico screening.
Ji-Dong, Shen   +8 more
openaire   +2 more sources

Elucidation of d-allulose recognition mechanism in ketose 3-epimerase

Journal of Bioscience and Bioengineering
d-Allulose is a low-calorie sweetener with multiple nutritional functions that can be produced through d-fructose isomerization by ketose 3-epimerase (KEase). l-Ribulose 3-epimerase from Arthrobacterglobiformis (AgLRE) is one of the most important enzymes that produce d-allulose; however, its substrate recognition mechanism is unknown.
Masahiro Watanabe   +2 more
openaire   +2 more sources

Enhancement of the d-Allulose 3-Epimerase Expression in Bacillus subtilis through Both Transcriptional and Translational Regulations.

Journal of Agricultural and Food Chemistry
d-Allulose, a functional bulk sweetener, has recently attracted increasing attention because of its low-caloric-ness properties and diverse health effects.
Wenli Zhang   +5 more
semanticscholar   +1 more source

Enhancing enzyme immobilization: Fabrication of biosilica-based organic-inorganic composite carriers for efficient covalent binding of D-allulose-3-epimerase.

International Journal of Biological Macromolecules
D-allulose, an ideal low-calorie sweetener, is primarily produced through the isomerization of d-fructose using D-allulose 3-epimerase (DAE; EC 5.1.3.30).
Ziqun Xiao   +3 more
semanticscholar   +1 more source

Simultaneous Enhancement of the Thermostability and Catalytic Activity of D-Allulose 3-Epimerase from Clostridium bolteae ATTC BAA-613 Based on the "Back to Consensus Mutations" Hypothesis.

Journal of Agricultural and Food Chemistry
D-Allulose is a high value rare sugar with multiple physiological functions and commercial potential that can be enzymatically synthesized from D-fructose by D-allulose 3-epimerase (DAEase).
Zhiqi Wang   +5 more
semanticscholar   +1 more source

Enhancing Stability and Catalytic Activity of d-Allulose 3-Epimerase through Multistrategy Computational Design and Cross-Regional Advantageous Mutations.

Journal of Agricultural and Food Chemistry
d-Allulose 3-epimerase (DAEase) derived from Clostridium bolteae has excellent properties in the catalytic production of d-allulose, a rare sugar with unique biological functions. However, the industrial application of C. bolteae DAEase (Cb-DAEase) for d-
Zhiqi Wang   +4 more
semanticscholar   +1 more source

Bioproduction of Rare d-Allulose from d-Glucose via Borate-Assisted Isomerization.

Journal of Agricultural and Food Chemistry
d-Allulose is a low-calorie functional rare sugar with excellent processing suitability and unique physiological efficacy. d-Allulose is primarily produced from d-fructose through enzymatic epimerization, facing the constraints of a low conversion yield ...
Xiaofang Xie, Dejian Huang, Zhaofeng Li
semanticscholar   +1 more source

Rational design improves both thermostability and activity of a new D-tagatose 3-epimerase from Kroppenstedtia eburnean to produce D-allulose.

Enzyme and Microbial Technology
D-allulose is a naturally occurring rare sugar and beneficial to human health. However, the efficient biosynthesis of D-allulose remains a challenge. Here, we mined a new D-tagatose 3-epimerase from Kroppenstedtia eburnean (KeDt3e) with high catalytic ...
Dingyu Guo   +10 more
semanticscholar   +1 more source

Bioproduction of D‐allulose: Properties , applications, purification, and future perspectives

Comprehensive Reviews in Food Science and Food Safety, 2021
Mengli Li, Bo Jiang, Tao Zhang
exaly  

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