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Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose

Enzyme and Microbial Technology, 2021
Abstract • Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g ...
Yi-Ting Jin, Z-Q Liu, Yu-Guo Zheng
exaly   +3 more sources

Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose

Food Chemistry, 2023
D-Allulose, as low-calorie rare sugar, possessed several notable biological activities and was biosynthesized by D-allulose 3-epimerase (DAEase). Here, CcDAE from Clostridium cellulolyticum was successfully immobilization via covalent attachment (RI-CcDAE), and Resin-SpyCatcher/SpyTag-CcDAE modular (DI-CcDAE).
Xin, Gao   +5 more
exaly   +3 more sources

Characterization of a Recombinant d-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in d-Allulose Production

Molecular Biotechnology, 2021
In recent years, with the increasing public health awareness, low-calorie rare sugars have received more attention on a global scale. D-Allulose, the C-3 epimer of D-fructose, is a representative rare sugar. It displays high sweetness and excellent physiological functions, but only provides a caloric value of 0.4 kcal/g. D-Allulose 3-epimerase (DAEase)
Jiajun Chen   +6 more
openaire   +2 more sources

Role of ‘D-allulose’ in a starch based composite gel matrix

open access: yesCarbohydrate Polymers, 2020
Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage. In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy ...
Esmanur Ilhan   +2 more
exaly   +4 more sources

D-allulose 3-epimerase for low-calorie D-allulose synthesis: microbial production, characterization, and applications

Critical Reviews in Biotechnology
D-allulose, an epimer of D-fructose at C-3 position, is a low-calorie rare sugar with favorable physiochemical properties and special physiological functions, which displays promising perspectives in the food and pharmaceutical industries. Currently, D-allulose is extremely sparse in nature and is predominantly biosynthesized through the isomerization ...
Zhaofeng Li
exaly   +3 more sources

d-Allulose enhances postprandial fat oxidation in healthy humans

Nutrition, 2017
d-Allulose, a C-3 epimer of d-fructose, has been reported to decrease body weight and adipose tissue weight in animal studies and is expected to be a potent antiobese sweetener. Our animal study suggested that one of the mechanisms of d-allulose's antiobesity function is an increase in energy expenditure.
Tomonori, Kimura   +6 more
openaire   +2 more sources

D-allulose, a versatile rare sugar: recent biotechnological advances and challenges

Critical Reviews in Food Science and Nutrition, 2021
D-Allulose is the C-3 epimer of D-fructose, and widely regarded as a promising substitute for sucrose. It's an excellent low-calorie sweetener, with 70% sweetness of sucrose, 0.4 kcal/g dietary energy, and special physiological functions. It has been approved as GRAS by the U.S. Food and Drug Administration, and is allowed to be excluded from total and
Wenli Zhang   +7 more
openaire   +2 more sources

Insulin mimetic effect of D‐allulose on apolipoprotein A‐I gene

Journal of Food Biochemistry, 2022
Several nutrients modulate the transcriptional activity of the apolipoprotein A-I (apo A-I) gene. To determine the influence of rare sugars on apo A-I expression in hepatic (HepG2) and intestinal derived (Caco-2) cell lines, apo A-I, albumin, and SP1 were quantified with enzyme immunoassay and Western blots while mRNA levels were quantified with real ...
Michael J. Haas   +7 more
openaire   +2 more sources

Bioproduction of D‐allulose: Properties , applications, purification, and future perspectives

Comprehensive Reviews in Food Science and Food Safety, 2021
Abstract D‐allulose is the C‐3 epimer of D‐fructose, which rarely exists in nature, and can be biosynthesized from D‐fructose by the catalysis of D‐psicose 3‐epimerase. D‐allulose is safe for human consumption and was recently approved by the United States Food and Drug Administration for food applications. It is not only able be used
Mengying Hu   +3 more
openaire   +2 more sources

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