Results 111 to 120 of about 3,123 (186)
Dcp2 Decaps m2,2,7GpppN-Capped RNAs, and Its Activity Is Sequence and Context Dependent†
, 2005 Hydrolysis of the mRNA cap plays a pivotal role in initiating and completing mRNA turnover. In nematodes, mRNA metabolism and cap-interacting proteins must deal with two populations of mRNAs, spliced leader trans-spliced mRNAs with a trimethylguanosine ...
Richard E. Davis
core
Biology Open, 2016 The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies.
Susanne Huch +5 more
doaj +1 more source
Down-regulation of Dcp2 mediates chronic nicotine-exposure-induced locomotor hyperactivity.
, 2012 A–C and F. Total RNA extracted from the heads of male flies was analyzed for Dcp2 or Dcp1 mRNA levels by relative qPCR. mRNA levels of the tested genes were normalized to rp49 mRNA. A.
Jing Ren (107777) +6 more
core +1 more source
Structural and Enzymatic Characterization of the Yeast mRNA Decapping Enzyme, Dcp2 [PDF]
, 2010 mRNA turnover represents a fundamental point of post-transcriptional control of gene expression in eukaryotes. Decapping is a highly regulated, irreversible step in mRNA decay and is involved in many different decay pathways.
Jones, Brittnee Noelle
core
Application of Mammalian Nudix Enzymes to Capped RNA Analysis
PharmaceuticalsFollowing the success of mRNA vaccines against COVID-19, mRNA-based therapeutics have now become a great interest and potential. The development of this approach has been preceded by studies of modifications found on mRNA ribonucleotides that influence ...
Maciej Lukaszewicz
doaj +1 more source
DCP1A, a MEK substrate, regulates the self-renewal and differentiation of mouse embryonic stem cells
Cell ReportsSummary: Mitogen-activated extracellular signal-regulated kinase (MEK) inhibitors are widely applied to maintain pluripotency, while prolonged MEK inhibition compromises the developmental potential of mouse embryonic stem cells (ESCs).
Jiayu Yu +12 more
doaj +1 more source
Conformational landscape and active conformation of the Dcp1:Dcp2 mRNA decapping complex
, 2017 Enzymes are dynamic molecular machines. Many insights into the molecular details of their function have been gained from crystal structures. But in the case of highly dynamic enzymes crystal structures are prone to packing artifacts.
Holdermann, I. +4 more
core
Loss of DCP2 impairs GAL1 transcript accumulation when induced from derepressive conditions.
, 2013 (A–C) Extended time course for analysis of GAL7 (A), GAL10 (B), and GAL1 (C) induction from derepressed conditions in dcp2Δ cells. Wild-type and dcp2Δ cells were grown in raffinose as above and were shifted to galactose to induce transcription of the GAL
Sara C. Cloutier (487136) +4 more
core +1 more source
Expression and immunolocalization of EhXRN2, EhDCP2 and EhCAF1.
, 2012 (A–B) Expression of 6xHis-tagged EhXRN2 (A) and EhDCP2 (B) proteins. Bacterial proteins were separated by 12% SDS-PAGE and the gels were stained with Coomassie blue. Lane 1: non-induced bacterial extracts; lane 2: IPTG-induced bacterial extracts. Western
César López-Camarillo (90937) +9 more
core +1 more source
Stress induces DCP2 translation via a stalling-dependent mechanism
The integrated stress response (ISR) globally suppresses protein synthesis while selectively permitting translation of a small subset of stress-responsive mRNAs, many of which contain upstream or overlapping open reading frames (uORFs/oORFs).
Marilena Neff +5 more
core +1 more source