Results 91 to 100 of about 3,302 (208)

The DNA damage response acts as a safeguardagainst harmful DNA–RNA hybrids ofdifferent origins [PDF]

, 2019
Despite playing physiological roles in specific situations, DNA–RNA hybrids threat genome integrity. To investigate how cells do counteract spontaneous DNA–RNA hybrids, here we screen an siRNA library covering 240 human DNA damage response (DDR) genes ...
Aguilera López, Andrés, Barroso Ceballos, Sonia Inés, García Rubio, María Luisa, Gómez González, Belén, Herrera Moyano, Emilia, Muñoz Sánchez, Sergio   +5 more
core   +1 more source

Polyphosphate: The “Dark Matter” of Bacterial Chromatin Structure

Molecular Microbiology, Volume 123, Issue 3, Page 279-293, March 2025.
Polyphosphate condensates and their contributions to nucleoid structure. Polyphosphate condensates form in the ribosome‐depleted nucleoid region of bacterial cells, particularly under stress (in this case Pseudomonas aeruginosa undergoing nitrogen starvation; adapted with permission from (Racki et al. 2017)). This Perspective explores the emerging view
Lisa R. Racki, Lydia Freddolino
wiley   +1 more source

Biophysical characterization of the Escherichia coli RNA degradosome

, 2022
In Escherichia coli, post-transcriptional regulation is a tightly controlled process facilitated by a multi-enzyme complex, the RNA degradosome. The core components of the RNA degradosome consist of the endoribonuclease RNase E, the DEAD-box helicase RhlB, the glycolytic enzyme enolase, and the exoribonuclease PNPase. The main scaffold component of the
openaire   +1 more source

Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome [PDF]

Nucleic Acids Research, 2014
The endoribonuclease RNase E is a key enzyme in RNA metabolism for many bacterial species. In Escherichia coli, RNase E contributes to the majority of RNA turnover and processing events, and the enzyme has been extensively characterized as the central component of the RNA degradosome assembly.
Voss, Jarrod E, Luisi, Ben F, Hardwick, Steven W   +2 more
openaire   +4 more sources

Simulating bacterial transcription and translation in a stochastic pi-calculus [PDF]

, 2006
International audienceStochastic simulation of genetic networks based on models in the stochastic pi-calculus is a promising recent approach. This paper contributes an extensible model of the central mechanisms of gene ex- pression i.e. transcription and
Kuttler, Celine
core   +1 more source

Membrane-association of mRNA decapping factors is independent of stress in budding yeast [PDF]

, 2016
Recent evidence has suggested that the degradation of mRNA occurs on translating ribosomes or alternatively within RNA granules called P bodies, which are aggregates whose core constituents are mRNA decay proteins and RNA.
A Hunt, A van Hoof, A-C Gavin, AE Frazier, AH Becker, AS Madrid, AS Spirin, C Kilchert, CB Brachmann, CJ Beckham, CJ Decker, D Teixeira, DA Mangus, DJ Gibbings, E Vergés, GM Rubin, H Strahl, H Zhang, J Coller, J LaCava, J Schindelin, J-H Yoon, JA Boon den, JA Toretsky, JE Hesketh, JE Wilhelm, K Shahbabian, KA Burke, KJ Bandyra, KJ Holmes, KM Kuhn, L Hatfield, L Huang, L Stalder, L Stalder, LEA Holmes, M Brengues, MP Ashe, N Cougot, R Parker, S Ghaemmaghami, S Huch, S Jagannathan, S Jonas, S Kadaba, S Kroschwald, T Nissan, T Nissan, T Sweet, TJ Sweet, U Sheth, V Balagopal, V Jouannet, V Khemici, V Pelechano, W Hu, W-K Huh, X Wang, Y Lin, Y Uesono, YS Lee, Z Li   +61 more
core   +1 more source

Effects of lipid membranes on RNA catalytic activity and stability

Biology of the Cell, Volume 117, Issue 2, February 2025.
This study explores how lipid membranes influence the activity and stability of natural ribozymes. We found that lipid membranes can both decrease and enhance ribozyme activity. We also observed that lipid membranes promote RNA degradation, depending on lipid composition and membrane fluidity.
Tomasz Czerniak, James P. Saenz
wiley   +1 more source

Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa [PDF]

, 2017
Polyphosphate (polyP) granule biogenesis is an ancient and ubiquitous starvation response in bacteria. Although the ability to make polyP is important for survival during quiescence and resistance to diverse environmental stresses, granule genesis is ...
Dieterle, Michael G., Jensen, Grant J., Newman, Dianne K., Racki, Lisa R., Sullivan, Meaghan C., Tocheva, Elitza I.   +5 more
core   +1 more source

A bacterial secreted translocator hijacks riboregulators to control type III secretion in response to host cell contact.

PLoS Pathogens, 2019
Numerous Gram-negative pathogens use a Type III Secretion System (T3SS) to promote virulence by injecting effector proteins into targeted host cells, which subvert host cell processes.
Maria Kusmierek, Jörn Hoßmann, Rebekka Witte, Wiebke Opitz, Ines Vollmer, Marcel Volk, Ann Kathrin Heroven, Hans Wolf-Watz, Petra Dersch   +8 more
doaj   +1 more source

Flotillins in membrane trafficking and physiopathology

Biology of the Cell, Volume 117, Issue 1, January 2025.
Flotillin 1 and 2 scaffold sphingolipid‐rich membrane microdomains involved in endocytosis and endolysosome maturation through the generation of sphingosine 1‐phosphate (S1P) by sphingosine kinase 2 (SphK2). Hence they deregulate protein cargo trafficking and influence cell signaling and cellular invasion.
Stéphane Bodin, Hadeer Elhabashy, Ewan Macdonald, Dominic Winter, Cécile Gauthier‐Rouvière   +4 more
wiley   +1 more source