Results 71 to 80 of about 3,184 (153)

Composition and adpatation of the E. coli RNA degradosome

, 2004
Although transcription and translation are recognized mechanisms for regulating gene expression, control over RNA stability can also accomplish this task. In E.
Prud’Homme Genereux, Annie
core   +1 more source

Flotillins in membrane trafficking and physiopathology

Biology of the Cell, Volume 117, Issue 1, January 2025.
Flotillin 1 and 2 scaffold sphingolipid‐rich membrane microdomains involved in endocytosis and endolysosome maturation through the generation of sphingosine 1‐phosphate (S1P) by sphingosine kinase 2 (SphK2). Hence they deregulate protein cargo trafficking and influence cell signaling and cellular invasion.
Stéphane Bodin, Hadeer Elhabashy, Ewan Macdonald, Dominic Winter, Cécile Gauthier‐Rouvière   +4 more
wiley   +1 more source

Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome.

, 2020
The method of co-immunoprecipitation (co-IP or pulldown) enables the identification of proteins interacting in macromolecular assemblies, through the purification of a key protein by affinity chromatography using specific antibodies immobilized on a ...
Ben F. Luisi, Steven W. Hardwick, Marilis V. Marques   +2 more
core   +2 more sources

The contribution of mRNA targeting to spatial protein localization in bacteria

The FEBS Journal, Volume 291, Issue 21, Page 4639-4659, November 2024.
mRNA‐based targeting serves as a back‐up strategy for the canonical signal sequence‐based protein targeting in bacteria. While signal sequence‐based targeting depends on dedicated protein‐targeting factors, mRNAs encoding inner membrane proteins can bind directly to protein translocases.
Wenkang Shang, Elisabeth Lichtenberg, Andreea Mihaela Mlesnita, Annegret Wilde, Hans‐Georg Koch   +4 more
wiley   +1 more source

Cyanobacterial RNA Helicase CrhR Localizes to the Thylakoid Membrane Region and Cosediments with Degradosome and Polysome Complexes in Synechocystis sptrain PCC 6803

, 2016
The cyanobacterium Synechocystis sp. strain PCC 6803 encodes a single DEAD box RNA helicase, CrhR, whose expression is tightly autoregulated in response to cold stress.
Whitford, Denise S., Owttrim, George W., Fahlman, Richard P., Rosana, Albert R. R.   +3 more
core   +1 more source

Biomolecular Condensates: From Bacterial Compartments to Incubator Spaces of Emergent Chemical Systems in Matter‐to‐Life Transitions

ChemSystemsChem, Volume 6, Issue 4, July 2024.
This review examines discoveries of biomolecular condensates as compartments in bacteria. Insights from bacterial cell biology and systems chemistry reveal intricate connections between chemical reactions and the control of condensate phase separation.
Wade E. Schnorr, W. Seth Childers
wiley   +1 more source

The DEAD-box RNA helicase CshA is required for fatty acid homeostasis in Staphylococcus aureus.

PLoS Genetics, 2020
Staphylococcus aureus is an opportunistic pathogen that can grow in a wide array of conditions: on abiotic surfaces, on the skin, in the nose, in planktonic or biofilm forms and can cause many type of infections. Consequently, S.
Vanessa Khemici, Julien Prados, Bianca Petrignani, Benjamin Di Nolfi, Elodie Bergé, Caroline Manzano, Caroline Giraud, Patrick Linder   +7 more
doaj   +1 more source

Escherichia coli strains with precise domain deletions in the ribonuclease RNase E can achieve greatly enhanced levels of membrane protein production

Protein Science, Volume 33, Issue 2, February 2024.
Abstract Escherichia coli is one of the most widely utilized hosts for production of recombinant membrane proteins (MPs). Bacterial MP production, however, is usually accompanied by severe toxicity and low‐level volumetric accumulation. In previous work, we had discovered that co‐expression of RraA, an inhibitor of the RNA‐degrading activity of RNase E,
Eleni Vasilopoulou, Tania Chroumpi, Georgios Skretas   +2 more
wiley   +1 more source

Pseudomonas aeruginosa enolase influences bacterial tolerance to oxidative stresses and virulence

Frontiers in Microbiology, 2016
Pseudomonas aeruginosa is a Gram negative opportunistic pathogenic bacterium, which causes acute and chronic infections. Upon entering host, bacteria alter global gene expression to adapt to host environment and avoid clearance by host.
Yuding Weng, Fei Chen, Yiwei Liu, Qiang Zhao, Xiaolei Pan, Chang Liu, Ronghao Chen, Zhihui Cheng, Shouguang Jin, Shouguang Jin, Yongxin Jin, Weihui Wu   +11 more
doaj   +1 more source

Recent insights into the world of dual‐function bacterial sRNAs

WIREs RNA, Volume 15, Issue 1, January/February 2024.
Dual‐function sRNAs are fascinating riboregulators that act both as base‐pairing antisense RNAs and as peptide‐coding mRNAs, offering the possibility to control target function at different regulatory levels via RNA–RNA interactions as well as protein–protein interactions.
Sebastian Benjamin Schnoor, Peter Neubauer, Matthias Gimpel   +2 more
wiley   +1 more source