Advanced Science, EarlyView.This study identifies FXYD1 as an epigenetically silenced tumor suppressor in breast cancer. DNA methylation turns off the gene FXYD1 in breast cancer, and low levels predict worse outcomes. Restoring FXYD1 limits breast cancer cells proliferation and metastasis. In the nucleus, FXYD1 recruits the E3 ligase MAEA to K63‐ubiquitinate DDX5 for proteasomal
Ping Wen +11 more
wiley +1 more source
DNA methylation modification is associated with gonadal differentiation in Monopterus albus
Cell & Bioscience, 2020 Background Both testis and ovary can be produced sequentially in an individual with the same genome when sex reversal occurs in the teleost Monopterus albus, and epigenetic modification is supposed to be involved in gonadal differentiation.
Xin Wang +6 more
doaj +1 more source
TET2 inhibits differentiation of embryonic stem cells but does not overcome methylation-induced gene silencing [PDF]
, 2014 TET2 is a methylcytosine dioxygenase that is frequently mutated in myeloid malignancies, notably myelodysplasia and acute myeloid leukemia. TET2 catalyses the conversion of 5′-methylcytosine to 5′-hydroxymethylcytosine within DNA and has been implicated ...
Chevassut, Timothy +2 more
core +3 more sources
One‐Step Glycoengineering of NK Cells With High‐Affinity Siglec Ligands for Cancer Immunotherapy
Advanced Science, EarlyView.This study develops an engineered NmCSS enzyme with improved activity for bulky group modified sialic acids, enabling efficient synthesis of structurally diverse sialoside analogs in a single step. Glycan microarray screening identified Siglec high‐affinity ligands, which were incorporated onto natural killer (NK) cells via a one‐pot enzymatic system ...
Shuai Hu +8 more
wiley +1 more source
PLoS ONE, 2020 Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya +8 more
doaj +1 more source
Newly synthesized APOBEC3G is incorporated into HIV virions, inhibited by HIV RNA, and subsequently activated by RNase H. [PDF]
PLoS Pathogens, 2007 APOBEC3G (A3G) is a potent antiretroviral deoxycytidine deaminase that, when incorporated into HIV virions, hypermutates nascent viral DNA formed during reverse transcription.
Vanessa B Soros +2 more
doaj +1 more source
Selective in vivo damage by "visible" light of BrdU-containing mitochondrial DNA in a thymidine kinase-deficient mouse cell line with persistent mitochondrial enzyme activity [PDF]
, 1975 The selective incorporation of 5-bromodeoxyuridine (BrdU) into mitochondrial DNA (mit-DNA) in the LM(TK-) Cl ID cell line, a thymidine kinase-deficient derivative of L, fibrobla8ts with persistent mitochondrial enzyme activity, has been utilized to ...
Attardi, G., Croizat, B.
core
FHL2 regulates hematopoietic stem cell functions under stress conditions. [PDF]
, 2015 FHL2, a member of the four and one half LIM domain protein family, is a critical transcriptional modulator. Here, we identify FHL2 as a critical regulator of hematopoietic stem cells (HSCs) that is essential for maintaining HSC self-renewal under ...
Chen, J +12 more
core +2 more sources
Polyelectrolyte Design Principles for Electrophoretic Drug Delivery
Advanced Science, EarlyView.Structure–property–function mapping of polyelectrolytes reveals how composition and nanoscale order control on‐demand electrophoretic drug transport. Charge density quantified under implant‐relevant conditions shows that encapsulation limits swelling, enabling high effective charge density.
Helena Saarela Unemo +8 more
wiley +1 more source
Total DNA methylation in the brain in response to decitabine treatment in female rats
European Pharmaceutical Journal, 2019 Hypomethylating agent decitabine is being used in the treatment of certain types of leukaemia in combination with other anticancer drugs. Aberrant DNA methylation has been suggested to occur in pathological states including depression.
Balagova L. +3 more
doaj +1 more source