Results 11 to 20 of about 67,632 (263)

FibroPrep protocol for rapid enzyme-free isolation of primary dermal fibroblasts from adult mouse skin for biological assays [PDF]

STAR Protocols
Summary: Isolating primary dermal fibroblasts is often costly, time-consuming, has high contamination risk, and is inefficient, yielding low cell numbers and requiring long culture periods.
Shubhangi Chakraborty, Supratim Pradhan, Deneshraj Srinivasan, Budhaditya Mukherjee   +3 more
doaj   +2 more sources

The weakness of senescent dermal fibroblasts

Proceedings of the National Academy of Sciences, 2023
Skin is the largest human organ with easily noticeable biophysical manifestations of aging. As human tissues age, there is chronological accumulation of biophysical changes due to internal and environmental factors. Skin aging leads to decreased elasticity and the loss of dermal matrix integrity via degradation.
Lydia Rebehn, Samira Khalaji, Fenneke KleinJan, Anja Kleemann, Fabian Port, Patrick Paul, Constantin Huster, Ulla Nolte, Karmveer Singh, Lisa Kwapich, Jonas Pfeil, Taner Pula, Pamela Fischer-Posovszky, Karin Scharffetter-Kochanek, Kay-E. Gottschalk   +14 more
openaire   +2 more sources

Enhancement Of Dermal Fibroblast Isolation Method [PDF]

Serbian Journal of Experimental and Clinical Research, 2015
ABSTRACT Cultivated fibroblasts have been widely used in a large number of in vitro studies. Although they readily proliferate under cell culture conditions, improvements in methods for their isolation are necessary. Here, we present our modified enzyme digestion method and compare its efficiency with commonly used techniques.
Zaric, Milan, Nikolic I., Zelen I., Mitrovic M., Milosavljevic Z.   +4 more
openaire   +2 more sources

Involvement of Wnt Signaling in Dermal Fibroblasts [PDF]

The American Journal of Pathology, 2010
Pachydermoperiostosis (PDP) is a rare disease characterized by unique phenotypes of the skin and bone, such as thick skin, implying that it may be caused by dysregulation of mesenchymal cells. The aim of this study is to examine the roles of dermal fibroblasts in the pathogenesis of pachydermia in association with Wnt signaling. The numbers of cultured
Kenji, Kabashima, Jun-Ichi, Sakabe, Ryutaro, Yoshiki, Yasuhiko, Tabata, Kimitoshi, Kohno, Yoshiki, Tokura   +5 more
openaire   +2 more sources

Sphingolipids control dermal fibroblast heterogeneity

Science, 2022
Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, we measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling ...
Laura Capolupo, Irina Khven, Alex R. Lederer, Luigi Mazzeo, Galina Glousker, Sylvia Ho, Francesco Russo, Jonathan Paz Montoya, Dhaka R. Bhandari, Andrew P. Bowman, Shane R. Ellis, Romain Guiet, Olivier Burri, Johanna Detzner, Johannes Muthing, Krisztian Homicsko, François Kuonen, Michel Gilliet, Bernhard Spengler, Ron M. A. Heeren, G. Paolo Dotto, Gioele La Manno, Giovanni D’Angelo   +22 more
openaire   +5 more sources

Dermal fibroblast in cutaneous development and healing [PDF]

WIREs Developmental Biology, 2017
The skin is the largest organ of the body and is composed of two layers: the overlying epidermis and the underlying dermis. The dermal fibroblasts originate from distinct locations of the embryo and contain the positional identity and patterning information in the skin.
Venkata, Thulabandu, Demeng, Chen, Radhika P, Atit   +2 more
openaire   +2 more sources

Involvement of Aquaporin 1 in the Motility and in the Production of Fibrillin 1 and Type I Collagen of Cultured Human Dermal Fibroblasts

Cosmetics, 2022
Aminocarbonyl proteins increase with age in the dermal layer. Gene Chip analysis of mRNA expression in human dermal fibroblasts cultured on collagen gels treated with glyceraldehyde as an aminocarbonyl protein and on untreated collagen gels showed a ...
Kazuhisa Maeda, Shiori Yoshida
doaj   +1 more source

Calcification in dermal fibroblasts from a patient with GGCX syndrome accompanied by upregulation of osteogenic molecules.

PLoS ONE, 2017
Gamma-glutamyl carboxylase (GGCX) gene mutation causes GGCX syndrome (OMIM: 137167), which is characterized by pseudoxanthoma elasticum (PXE)-like symptoms and coagulation impairment.
Yumi Okubo, Ritsuko Masuyama, Akira Iwanaga, Yuta Koike, Yutaka Kuwatsuka, Tomoo Ogi, Yosuke Yamamoto, Yuichiro Endo, Hiroshi Tamura, Atsushi Utani   +9 more
doaj   +1 more source

Murine Dermal Fibroblast Isolation by FACS [PDF]

Journal of Visualized Experiments, 2016
Fibroblasts are the principle cell type responsible for secreting extracellular matrix and are a critical component of many organs and tissues. Fibroblast physiology and pathology underlie a spectrum of clinical entities, including fibroses in multiple organs, hypertrophic scarring following burns, loss of cardiac function following ischemia, and the ...
Graham G, Walmsley, Zeshaan N, Maan, Michael S, Hu, David A, Atashroo, Alexander J, Whittam, Dominik, Duscher, Ruth, Tevlin, Owen, Marecic, H Peter, Lorenz, Geoffrey C, Gurtner, Michael T, Longaker   +10 more
openaire   +2 more sources

DETERMINATION OF BIOCOMPATIBILITY AND CYTOTOXICITY OF POROUS TITANIUM-BASED MATERIALS IN EXPERIMENT

Наука и инновации в медицине, 2017
Aim - to evaluate the proliferative activity of dermal fibroblast cultures in the presence of composite materials based on titanium silicides in vitro. Materials and methods.
AV V Kolsanov, AN N Nikolaenko, VV V Ivanov, SA A Prikhodko, PV V Platonov   +4 more
doaj   +1 more source