Results 1 to 10 of about 216 (112)

Basis for rapid efflux of biosynthetic desmosterol from cells [PDF]

Journal of Lipid Research, 1998
Previous work shows that the efflux of biosynthetic desmosterol from cells is three times more efficient than that of cholesterol. To explain this difference, we labeled CHO-K1 cells with [3H]acetate precursor and measured sterols in the whole cells ...
Jane Ellen Phillips, Wendi V. Rodrigueza, William J. Johnson   +2 more
doaj   +3 more sources

Oxygenation of desmosterol and cholesterol in cell cultures.

Journal of Lipid Research, 1990
In order to determine whether hydration of the delta 24 bond of desmosterol contributes to the formation of the regulatory oxysterol, 25-hydroxycholesterol, [3H]desmosterol was incubated with two cultured cell lines and the labeled products were analyzed.
SE Saucier, AA Kandutsch, AK Gayen, JA Nelson, TA Spencer   +4 more
doaj   +3 more sources

Desmosterolosis and desmosterol homeostasis in the developing mouse brain

Journal of Inherited Metabolic Disease, 2019
AbstractCholesterol serves as a building material for cellular membranes and plays an important role in cellular metabolism. The brain relies on its own cholesterol biosynthesis, which starts during embryonic development. Cholesterol is synthesized from two immediate precursors, desmosterol and 7‐dehydrocholesterol (7‐DHC).
Thiago C. Genaro-Mattos, Ned A. Porter, Karoly Mirnics   +2 more
exaly   +4 more sources

The influence of cholesterol precursor – desmosterol – on artificial lipid membranes

Biochimica Et Biophysica Acta - Biomembranes, 2015
The disorders in cholesterol biosynthesis pathway and various diseases manifest in the accumulation of cholesterol precursors in the human tissues and cellular membranes. In this paper the effect of desmosterol--one of cholesterol precursors--on model lipid membranes was studied.
Michał Flasiński, Paweł Wydro, Katarzyna Hąc-Wydro   +2 more
exaly   +4 more sources

Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility

Frontiers in Cell and Developmental Biology, 2021
Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol content.
María de las Mercedes Carro, Rafael R. A. Ramírez-Vasquez, Daniel A. Peñalva, Daniel A. Peñalva, Jorgelina Buschiazzo, Federico A. Hozbor   +5 more
doaj   +1 more source

Desmosterol in Human Nonalcoholic Steatohepatitis [PDF]

Hepatology, 2013
Dysregulation of the cholesterol synthesis pathway and accumulation of cholesterol in the liver are linked to the pathogenesis of nonalcoholic steatohepatitis (NASH). Therefore, we investigated the association of serum and liver levels of cholesterol precursors with NASH.
Kaminska Dorota, Gylling Helena Kristiin, Kuusisto Johanna, Pihlajamäki Jussi, Laakso Markku, Simonen Marko, Käkelä Pirjo, Kärjä Vesa Juhani, Männistö Ville, Gylling Helena, Pihlajamäki Jussi, Kuusisto Maria, Laakso Markku, Simonen Marko, Käkelä Pirjo, Venesmaa Sari, Kärjä Vesa, Männistö Ville, Gylling Helena, Gylling Helena   +19 more
openaire   +2 more sources

Characterisation of a desmosterol reductase involved in phytosterol dealkylation in the silkworm, Bombyx mori. [PDF]

PLoS ONE, 2011
Most species of invertebrate animals cannot synthesise sterols de novo and many that feed on plants dealkylate phytosterols (mostly C(29) and C(28)) yielding cholesterol (C(27)). The final step of this dealkylation pathway involves desmosterol reductase (
Leonora F Ciufo, Patricia A Murray, Anu Thompson, Daniel J Rigden, Huw H Rees   +4 more
doaj   +1 more source

Identification and Characterization of 24-Dehydrocholesterol Reductase (DHCR24) in the Two-Spotted Cricket, Gryllus bimaculatus

Insects, 2021
Arthropods, including insects, convert sterols into cholesterol due to the inability to synthesise cholesterol de novo. 24-dehydrocholesterol reductase (DHCR24) plays an important role in the conversion. Not only involving the cholesterol biosynthesis in
Yin Shan Isa Mack, Masatoshi Dehari, Nobukatsu Morooka, Shinji Nagata   +3 more
doaj   +1 more source

Generation and validation of a conditional knockout mouse model for desmosterolosis

Journal of Lipid Research, 2021
: The enzyme 3β-hydroxysterol-Δ24 reductase (DHCR24, EC 1.3.1.72) catalyzes the conversion of desmosterol to cholesterol and is obligatory for post-squalene cholesterol synthesis.
Babunageswararao Kanuri, Vincent Fong, Sithara Raju Ponny, Ranjuna Weerasekera, Kirthi Pulakanti, Kriya S. Patel, Roman Tyshynsky, Shailendra B. Patel   +7 more
doaj   +1 more source

Desmosterol accumulation in users of amiodarone [PDF]

Journal of Internal Medicine, 2017
AbstractBackgroundAmiodarone is an effective and widely used antiarrhythmic drug with many possible adverse effects including hypercholesterolaemia and hepatotoxicity.ObjectiveOur aim was to evaluate how long‐term amiodarone treatment affects cholesterol metabolism.MethodsThe study population consisted of 56 cardiac patients, of whom 20 were on ...
Lampi A. -M., Gylling H., Lehtonen J., Kupari M., Simonen P., Stenman U. -H., Piironen V., Lampi A. -M., Gylling H., Lehtonen J., Kupari M., Simonen P., Stenman U. -H., Piironen V.   +13 more
openaire   +2 more sources