Results 121 to 130 of about 691 (143)
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Inhibition of Dextransucrase by Excess Substrate
Nature, 1958HEHRE1, working with the enzyme dextransucrase, showed that a plot of initial velocity v against substrate concentration deviated from linearity above a sucrose concentration of 200 mM. This was tentatively explained on the basis of a decrease in concentration of water in the stronger sucrose solutions.
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Production of Leucrose by Dextransucrase
Starch - Stärke, 1998Dextransucrase can be used to synthesize the disaccharide leucrose from sucrose and fructose under appropriate reaction conditions. HPLC methods to analyze the reaction solutions and an enzyme activity test were developed to determine reaction kinetics and by-products in detail. As a result, a separate acceptor binding site at the enzyme was postulated
Klaus Buchholz +2 more
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Activation and inhibition of dextransucrase by calcium
Biochimica et Biophysica Acta (BBA) - General Subjects, 1986Initial rate kinetics of dextran synthesis by dextransucrase (sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, EC 2.4.1.5) from Leuconostoc mesenteroides NRRL B-512F showed that below 1 mM, Ca2+ activated the enzyme by increasing Vmax and decreasing the Km for sucrose. Above 1 mM, Ca2+ was a weak competitive inhibitor (Ki = 59 mM). Although it
A W, Miller, J F, Robyt
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Immobilization of dextransucrase on functionalized TiO2 supports
International Journal of Biological Macromolecules, 2018The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation ...
Miljković, Miona G. +8 more
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Oligosaccharide synthesis by dextransucrase: new unconventional acceptors
Carbohydrate Research, 2002The acceptor reactions of dextransucrase offer the potential for a targeted synthesis of a wide range of di-, tri- and higher oligosaccharides by the transfer of a glucosyl group from sucrose to the acceptor. We here report on results which show that the synthetic potential of this enzyme is not restricted to 'normal' saccharides.
Kristin, Demuth +2 more
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The hydrolysis of sucrose by dextransucrase
Carbohydrate Research, 1983Abstract Dextransucrase was shown to catalyze the hydrolysis of sucrose. The hydrolytic activity was found to be directly correlatable with dextransucrase activity on poly-(acrylamide) disc-gel electrophoresis. In studies on the hydrolysis of sucrose and formation of dextran as a function of time and substrate concentration, the two activities were ...
Gary A. Luzio, Robert M. Mayer
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Unconventional immobilization of dextransucrase with alginate
Enzyme and Microbial Technology, 1995Abstract An unconventional immobilization of dextransucrase via entrapment in alginate is described, which provides high activity yield and good operational stability. This method is normally restricted to the immobilization of complete cells or parts of cells. Experiments were designed to provide evidence for the immobilization mechanism.
Astrid Reischwitz +2 more
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Kinetics of leucrose formation from sucrose by dextransucrase
Biotechnology and Bioengineering, 1994AbstractLeucrose formation from sucrose and fructose by dextransucrase is of practical interest. It has been investigated at different experimental conditions, including the influence of temperature on reaction rate and selectivity. Under appropriate conditions high product yield can be obtained.
M, Böker, H J, Jördening, K, Buchholz
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Proteolytic modification of Leuconostoc mesenteroides B-512F dextransucrase
Antonie van Leeuwenhoek, 2005Multiple active lower molecular weight forms from Leuconostoc mesenteroides B512F dextransucrase have been reported. It has been suggested that they arise from proteolytic processing of a 170 kDa precursor. In this work, the simultaneous production of proteases and dextransucrase was studied in order to elucidate the dextransucrase proteolytic ...
Martha, Argüello-Morales +5 more
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Interpretation of dextransucrase inhibition at high sucrose concentrations
Carbohydrate Research, 1993When acceptor reactions were carried out at high sucrose concentrations (> or = 200 mM), dextran synthesis was inhibited and the acceptor reactions were increased. A model, based on the known mechanisms of dextran synthesis and acceptor reactions, is proposed to explain the inhibition of dextran synthesis and the increase in the acceptor products at ...
A, Tanriseven, J F, Robyt
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