Results 41 to 50 of about 691 (143)

Studies on Dextransucrase Part I

Journal of the agricultural chemical society of Japan, 1972
(1) Leucanostoc mesenteroides IAM 1046により,デキストランシュクラーゼを生産させるための培地,および培養条件を検討した. (2)上記菌株のデキストランシュクラーゼの部分的精製を行ない,硫安塩析およびDEAE-セルロースによるクロマトグラフィーを期いて,比活性が培養液の24～56倍の精製酵素を得た. (3)部分精製したデキストランシュクラーゼの酵素化学的性質を検討した.その結果,この酵素は作用最適pH 5.0,最適温度30°C, pH 4.0～7.5において安定であるが, 40°C以上にて速やかに失活するなどのことがわかった.またMichaelis恒数は30°C, pH 5.0で1.57×10-2 (M)であった.
Michio NIINOBE, Tsuneo KOBAYASHI
openaire   +2 more sources

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Mass Spectrometry Reviews, Volume 44, Issue 3, Page 213-453, May/June 2025.
Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022.
David J. Harvey
wiley   +1 more source

Purification and Characterization of an Extracellular Dextransucrase from Pediococcus pentosaceus Isolated from the Soil of North East India

Food Technology and Biotechnology, 2011
The extracellular dextransucrase produced from Pediococcus pentosaceus, a new isolate from the soil in Assam, India, was purified and characterized. The enzyme activity of cell-free supernatant was 3.4 U/mL and specific activity was 0.6 U/mg.
Damini Kothari, Seema Patel, Arun Goyal
doaj  

Recombinant Expression of Dextransucrase in Food-grade Lactic Acid Bacteria

Shipin gongye ke-ji
In order to study the synthesis of dextran in Lactobacillus and to analyze its structure, in this study, the dextransucrase DsrB was ligated with the Lactococcus lactis expression vector pNZ8148 plasmid vector with SP45 secretion signal peptide, and ...
Yuanhao PU, Kaige PENG, Xiaoyu LIU, Qingyong MENG, Fangkun ZHAO, Yaxin SANG   +5 more
doaj   +1 more source

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 21: Suitability of taxonomic units notified to EFSA until September 2024

EFSA Journal, Volume 23, Issue 1, January 2025.
Abstract The qualified presumption of safety (QPS) process was developed to provide a safety assessment approach for microorganisms intended for use in food or feed chains. In the period covered by this Statement, no new information was found that would change the status of previously recommended QPS taxonomic units (TUs).
EFSA Panel on Biological Hazards (BIOHAZ), Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panagiotis Skandamis, Elisabetta Suffredini, Marianne Chemaly, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Angela Botteon, Baptista Carolina, Barizzone Fulvio, Sandra Correia, Lieve Herman   +28 more
wiley   +1 more source

Hydroxyapatite: An Eco‐Friendly Material for Enzyme Immobilization

ChemPlusChem, Volume 89, Issue 8, August 2024.
This minireview provides a literature survey on the use of hydroxyapatite (HAP) as a sustainable and ecofriendly support for enzyme immobilization, a key technology for the design of stable, efficient and reusable biocatalysts. The different immobilization techniques used for obtaining biocatalysts based on HAP and HAP‐composites are considered and ...
Leonardo Gelati, Marco Rabuffetti, Maurizio Benaglia, Sebastiano Campisi, Antonella Gervasini, Giovanna Speranza, Carlo F. Morelli   +6 more
wiley   +1 more source

Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

Brazilian Archives of Biology and Technology, 2003
Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom.
Michelena Georgina L., Martínez Aidín, Bell Antonio, Carrera Emilia, Valencia Roxana   +4 more
doaj  

Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor

Brazilian Archives of Biology and Technology, 2011
The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type.
Seema Patel, Damini Kothari, Rishikesh Shukla, Debasish Das, Arun Goyal   +4 more
doaj   +1 more source

Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis

Advanced Materials, Volume 36, Issue 27, July 4, 2024.
Linear d‐glucans, simple yet versatile polysaccharides, shape nanostructures crucial for biomaterial development. Shifting from top‐down to bottom‐up synthesis using natural and engineered enzymes provides meticulous control over polymer structures, fostering diversification for macromolecular materials.
Chao Zhong, Bernd Nidetzky
wiley   +1 more source

Distribution of dextransucrase in Streptococcus mutans and observations on the effect of soluble dextran on dextransucrase activities [PDF]

Infection and Immunity, 1977
Total and insoluble dextransucrase activities were measured in cell-associated and supernatant fractions of Streptococcus mutans GS-5 grown in several media. Although the amount of cell-associated and supernatant activity varied greatly as a function of medium, the total activity appeared constant.
T J, Montville, C L, Cooney, A J, Sinskey   +2 more
openaire   +2 more sources