Results 91 to 100 of about 1,193,866 (323)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Digestible lysine levels in low-protein diets supplemented with synthetic amino acids for nursery, growing, and finishing barrows

, 2012
peer-reviewed articleTo investigate the effect of lysine level in low-protein diets (LPD) an experiment was conducted with 36 barrows fed 174, 140 and 118 g/kg of crude protein (CP), and three digestible lysine levels.
Sánchez-Torres, M. T., Zamora, V., Nieto, R., Figueroa, J. L., Copado, J. M. F., Estrada, J., Cordero, J. L.   +6 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Performance and nitrogen balance of laying hens fed increasing levels of digestible lysine and arginine

Revista Brasileira de Zootecnia, 2012
The objective of this experiment was to evaluate the effect of two digestible lysine levels and four digestible arginine levels on laying hens from 24 to 48 weeks of age.
Fabyola Barros de Carvalho, José Henrique Stringhini, Maíra Silva Matos, Roberto Moraes Jardim Filho, Marcos Barcellos Café, Nadja Susana Mogyca Leandro, Maria Auxiliadora Andrade   +6 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Different criteria of feed formulation for broilers aged 43 to 49 days

Brazilian Journal of Poultry Science, 2004
An experiment was carried out to evaluate different formulated feeds for broilers from 43 to 49 days of age. Six hundred male Cobb broilers were distributed in a completely at random experimental design with 3 treatments (3 different criteria of feed ...
LF Araújo, OM Junqueira, CSS Araújo, DE Faria, MO Andreotti   +4 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase

Poultry Science, 2018
A total of 90 male Ross 308 broiler chicks were used in a digestibility and performance bioassay to explore the effect of reduction in dietary protein and digestible amino acids and inclusion of an exogenous mono‐component protease on amino acid ...
A. Cowieson, M. Toghyani, S. Kheravii, S. Wu, L. Romero, M. Choct   +5 more
semanticscholar   +1 more source

Effect of variety, growing region and growing season on digestible energy content of wheats grown in Western Australia for weaner pigs

, 2004
An experiment was conducted to examine the digestible energy (DE) content for weanling pigs in a cohort of wheats grown in Western Australia, and to establish relationships between DE content and their chemical composition.
Pluske, J.R., Mullan, B.P., Simmins, P.H., Kim, J.C.   +3 more
core  

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source