Results 131 to 140 of about 2,135 (155)

Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae

open access: yesBiochemical and Biophysical Research Communications, 2020
Cyclic-di-GMP (c-di-GMP) synthesized by diguanylate cyclases has been an important and ubiquitous secondary messenger in almost all bacterial systems. In Vibrio cholerae, c-di-GMP plays an intricate role in the production of the exopolysaccharide matrix,
Yvette Roske   +2 more
exaly   +4 more sources

Natural diversity provides a broad spectrum of cyanobacteriochrome-based diguanylate cyclases

open access: yesPlant Physiology, 2021
Abstract Cyanobacteriochromes (CBCRs) are spectrally diverse photosensors from cyanobacteria distantly related to phytochromes that exploit photoisomerization of linear tetrapyrrole (bilin) chromophores to regulate associated signaling output domains. Unlike phytochromes, a single CBCR domain is sufficient for photoperception.
Matthew Blain-Hartung   +2 more
exaly   +3 more sources

Structural analysis of an oxygen-regulated diguanylate cyclase

Acta Crystallographica Section D Biological Crystallography, 2015
Cyclic di-GMP is a bacterial second messenger that is involved in switching between motile and sessile lifestyles. Given the medical importance of biofilm formation, there has been increasing interest in understanding the synthesis and degradation of cyclic di-GMPs and their regulation in various bacterial pathogens.
Miroslaw Tarnawski   +2 more
openaire   +3 more sources

Characterization of a diguanylate cyclase from Shewanella woodyi with cyclase and phosphodiesterase activities

Molecular BioSystems, 2010
Abstract Cyclic-di-GMP signaling is used by many bacteria to control biofilm formation. As biofilm formation is an important survival strategy for many bacteria, the synthesis and degradation of cyclic-di-GMP is tightly regulated by enzymes containing domains with conserved GGDEF and EAL sequence motifs, respectively.
Niu, Liu, Taemee, Pak, Elizabeth M, Boon
openaire   +2 more sources

Structural characterization of a putative diguanylate cyclase conserved in hyperthermophiles

Biochemical and Biophysical Research Communications, 2019
C-di-GMP, bis-(3'-5')-cyclic dimeric guanosine monophosphate, is a key signaling molecule that regulates many important physiological processes in bacteria. C-di-GMP is synthesized by diguanylate cyclase (DGC) containing the homodimeric GGDEF domain. There are many uncharacterized hypothetical proteins annotated as a putative DGC in bacteria including ...
Kyung Wook Been   +3 more
openaire   +2 more sources

Bacterial diguanylate cyclases: Structure, function and mechanism in exopolysaccharide biofilm development

Biotechnology Advances, 2015
The ubiquitous bacterial cyclic di-guanosine monophosphate (c-di-GMP) emerges as an important messenger for the control of many bacterial cellular functions including virulence, motility, bioluminescence, cellulose biosynthesis, adhesion, secretion, community behaviour, biofilm formation and cell differentiation. The synthesis of this cyclic nucleotide
Duu-Jong Lee
exaly   +3 more sources

Expression, purification and characterization of diguanylate cyclase from Rhodococcus ruber

Protein Expression and Purification, 2019
Diguanylate cyclases (DGCs) were responsible for the synthesis of second messenger cyclic di-guanosine monophosphate (c-di-GMP), which were involved in various physiological activities of bacterial species. Here, a full-length DGC from Rhodococcus ruber SD3 fused with glutathione-S-transferase (GST) was expressed in E.
Sufang, Kuang   +3 more
exaly   +3 more sources

In Vitro Measurement of Gas-Dependent and Redox-Sensitive Diguanylate Cyclase Activity

2023
Bacteria sense and respond to gaseous ligand changes in the environment to regulate a multitude of behaviors, including the production of the secondary messengers cyclic di-GMP. Gas sensing can be difficult to measure due to the high concentration of the oxygen in the atmosphere, particularly in redox-sensitive systems.
Nushrat J, Hoque   +2 more
openaire   +2 more sources

Oligoribonuclease is a central feature of cyclic diguanylate signaling inPseudomonas aeruginosa

open access: yesProceedings of the National Academy of Sciences of the United States of America, 2015
International audienceThe second messenger cyclic diguanylate (c-di-GMP) controls diverse cellular processes among bacteria. Diguanylate cyclases synthesize c-di-GMP, whereas it is degraded by c-di-GMP-specific phosphodiesterases (PDEs).
Trevor E Randall   +2 more
exaly   +2 more sources

Determining Diguanylate Cyclase Activity (Radioactive Assay)

2017
Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.
openaire   +2 more sources

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