Results 91 to 100 of about 1,001 (132)

22R‐ but not 22S‐hydroxycholesterol is recruited for diosgenin biosynthesis

The Plant Journal, 2021
SUMMARYDiosgenin is an important compound in the pharmaceutical industry and it is biosynthesized in several eudicot and monocot species, herein represented by fenugreek (a eudicot), and Dioscorea zingiberensis (a monocot). Formation of diosgenin can be achieved by the early C22,16‐oxidations of cholesterol followed by a late C26‐oxidation.
Chen Zhou, Yuhui Yang, Jingyi Tian, Yihan Wu, Faliang An, Changfu Li, Yansheng Zhang   +6 more
openaire   +2 more sources

Metabolic engineering of the diosgenin biosynthesis pathway in Trigonella foenum-graceum hairy root cultures

Plant Science, 2022
Diosgenin as a triterpene with numbers of pharmaceutical applications has been identified in Trigonella foenum-graceum. In this survey, in order to scale up the amount of diosgenin in Fenugreek as a promising alternative of yam, ∆24-reductase as a rate limiting enzyme in diosgenin biosynthesis pathway has been overexpressed by utilizing pBI121 ...
Ahmad, Nasiri, Sajad, Rashidi-Monfared, Amin, Ebrahimi, Nargues, Falahi Charkhabi, Ahmad, Moieni   +4 more
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Biosynthesis of diosgenin in Dioscorea tubers

Archives of Biochemistry and Biophysics, 1961
Abstract The incorporation of radioacetate into diosgenin by homogenates of Dioscorea floribunda has been demonstrated by rigorous purification of the isolated sapogenin. Mevalonic acid is not incorporated into diosgenin to any significant extent under these conditions.
E, HEFTMANN, R D, BENNETT, J, BONNER
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Biosynthesis and Translocation of Diosgenin in Costus speciosus

Journal of Plant Physiology, 1987
Summary Radio-actively labelled free and conjugated diosgenin was isolated from different parts such as stem, leaf, seeds, flowers, and rhizomes of Costus speciosus after feeding 14C mevalonate, diosgenin, and diosgenin glycoside through different parts in separate sets of experiments.
Anand Akhila, Madan M. Gupta
openaire   +1 more source

Biosynthesis of diosgenin from 26-hydroxycholesterol in dioscorea floribunda

Phytochemistry, 1970
Abstract 26-Hydroxycholesterol-26- 14 C was converted by a Dioscorea floribunda plant to diosgenin. This result suggests that oxygenation at C-26 may be the first step in the biosynthesis of diosgenin from cholesterol. No diosgenin was formed from kryptogenin-26- 14 C by D.
Raymond D. Bennett, Erich Heftmann, Raymond A. Joly   +2 more
openaire   +1 more source

Deciphering the network of cholesterol biosynthesis in Paris polyphylla laid a base for efficient diosgenin production in plant chassis

Metabolic Engineering, 2023
Cholesterol serves as a key precursor for many high-value chemicals such as plant-derived steroidal saponins and steroidal alkaloids, but a plant chassis for effective biosynthesis of high levels of cholesterol has not been established. Plant chassis have significant advantages over microbial chassis in terms of membrane protein expression, precursor ...
Xue Yin, Jia Liu, Chengxi Kou, Jiaojiao Lu, He Zhang, Wei Song, Yuhua Li, Zheyong Xue, Xin Hua   +8 more
openaire   +2 more sources

[Pathway design and key enzyme analysis of diosgenin biosynthesis].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2021
As a naturally occurring steroid sapogenin, diosgenin acts as the precursor of hundreds of steroid medicines, and thereby has important medicinal value. Currently, industrial production of diosgenin relies primarily on chemical extraction from plant materials.
Zhongyi, Sun, Peng, Zhao, Xizhen, Ge, Pingfang, Tian   +3 more
openaire   +1 more source

Improving diosgenin production and its biosynthesis in Trigonella foenum-graecum L. hairy root cultures

Industrial Crops and Products, 2020
Abstract Induction of hairy roots stemmed from Agrobacterium rhizogenes transformation, is a key step to reach mass production of secondary metabolites in medicinal plants. Accordingly, this study is aimed to investigate potential impact of different A.
Farnaz Zolfaghari, Sajad Rashidi-Monfared, Ahmad Moieni, Davar Abedini, Amin Ebrahimi   +4 more
openaire   +1 more source