Results 1 to 10 of about 246,136 (236)

Active learning-assisted directed evolution. [PDF]

Nat Commun
Directed evolution (DE) is a powerful tool to optimize protein fitness for a specific application. However, DE can be inefficient when mutations exhibit non-additive, or epistatic, behavior.
Yang J, Lal RG, Bowden JC, Astudillo R, Hameedi MA, Kaur S, Hill M, Yue Y, Arnold FH.   +8 more
europepmc   +4 more sources

Directed Evolution of a Mycobacteriophage [PDF]

Antibiotics, 2019
Bacteriophages represent an alternative strategy to combat pathogenic bacteria. Currently, Mycobacterium tuberculosis infections constitute a major public health problem due to extensive antibiotic resistance in some strains.
María Cebriá-Mendoza, Rafael Sanjuán, Pilar Domingo-Calap   +2 more
doaj   +4 more sources

Directed evolution of hydrocarbon-producing enzymes. [PDF]

Biotechnol Biofuels Bioprod
Enzymes capable of catalysing the production of hydrocarbons hold promise for sustainable fuel synthesis. However, the native activities of these enzymes are often insufficient for their exploitation in industrial bioprocesses.
Nielsen JR, Kennerley J, Huang WE.
europepmc   +2 more sources

Directed evolution of drug-like Aβ conformation-specific antibodies. [PDF]

Front Immunol
Monoclonal antibodies that recognize conformational epitopes in protein aggregates are important for research, diagnostic, and therapeutic applications related to neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases. Unfortunately, it
Desai AA, Smith MD, Zupancic JM, Makowski EK, Zhang Y, Gerson JE, Moore SJ, Sutter AB, Ferris SP, Ivanova MI, Murphy GG, Paulson HL, Tessier PM.   +12 more
europepmc   +2 more sources

A chimeric viral platform for directed evolution in mammalian cells. [PDF]

Nat Commun
Directed evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Directed evolution platforms are primarily prokaryotic or yeast-based, and stable mammalian systems have been challenging to ...
Cole AJ, Denes CE, Moreno CL, Hunault L, Dobson T, Hesselson D, Neely GG.   +6 more
europepmc   +2 more sources

Optimisation strategies for directed evolution without sequencing. [PDF]

PLoS Comput Biol
Directed evolution can enable engineering of biological systems with minimal knowledge of their underlying sequence-to-function relationships. A typical directed evolution process consists of iterative rounds of mutagenesis and selection that are ...
James J, Towers S, Foerster J, Steel H.
europepmc   +2 more sources

Protein directed evolution

Revista de Química, 2008
Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational
Roberto Laos
doaj   +2 more sources

A risk based assessment approach for chemical mixtures from wastewater treatment plant effluents

Environment International, 2022
In this study, 56 effluent samples from 52 European wastewater treatment plants (WWTPs) were investigated for the occurrence of 499 emerging chemicals (ECs) and their associated potential risks to the environment.
Saskia Finckh, Liza-Marie Beckers, Wibke Busch, Eric Carmona, Valeria Dulio, Lena Kramer, Martin Krauss, Leo Posthuma, Tobias Schulze, Jaap Slootweg, Peter C. Von der Ohe, Werner Brack   +11 more
doaj   +1 more source

Cybernetics and Directed Evolution

Кібернетика та комп'ютерні технології, 2023
Results. The place and role of cybernetics methods for solving the global problem of directed evolution are considered. The author investigates the eventual phenomenon of the interaction of two coryphaei of Ukrainian science V.I.
Oleksandr Palagin
doaj   +1 more source

Directed Evolution Methods for Enzyme Engineering

Molecules, 2021
Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability.
Saurabh Rajendra Nirantar
doaj   +1 more source