Results 11 to 20 of about 246,136 (236)
Nature, 2021 Abstract Directed evolution can rapidly achieve dramatic improvements in the properties of a protein or bestow entirely new functions on it. We have discovered a strong correlation between the probability of finding a productive mutation at a particular position of a protein and a chemical shift perturbation in Nuclear Magnetic Resonance ...
Sagar Bhattacharya +10 more
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Directed polymerase evolution [PDF]
FEBS Letters, 2013 Polymerases evolved in nature to synthesize DNA and RNA, and they underlie the storage and flow of genetic information in all cells. The availability of these enzymes for use at the bench has driven a revolution in biotechnology and medicinal research; however, polymerases did not evolve to function efficiently under the conditions required for some ...
Chen, Tingjian, Romesberg, Floyd E.
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RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
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Models for the directed evolution of bacterial allelopathy: bacteriophage lysins [PDF]
PeerJ, 2015 Microbes produce a variety of compounds that are used to kill or suppress other species. Traditional antibiotics have their origins in these natural products, as do many types of compounds being pursued today in the quest for new antibacterial drugs ...
James J. Bull +3 more
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Towards the Directed Evolution of Artificial Metalloenzymes
CHIMIA, 2021 Artificial metalloenzymes (ArMs) are a class of enzymes holding great promise. In contrast to natural enzymes, the core of ArMs is a synthetic metallocofactor, with potential for bio-orthogonal reactivity, incorporated within a host protein.
Jaicy Vallapurackel
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Directing Drug Evolution [PDF]
BioTechniques, 2017 Directed evolution is poised to change small molecule discovery and provide greater access to “drug space.” Sarah Webb looks into the evolving drug discovery landscape.
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Directed evolution: selection of the host organism
Computational and Structural Biotechnology Journal, 2012 Directed evolution has become a well-established tool for improving proteins and biological systems. A critical aspect of directed evolution is the selection of a suitable host organism for achieving functional expression of the target gene.
Azadeh Pourmir, Tyler W Johannes
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Evolution of a Student Directed Free Clinic
Journal of Regional Medical Campuses, 2023 This report summarizes the 20 year evolution of community engagement at a small regional rural campus. The process includes establishing a student directed free clinic and its transition through the wider availability of Medicaid expansion.
William J. Crump +3 more
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Recent Advances in Directed Yeast Genome Evolution
Journal of Fungi, 2022 Saccharomyces cerevisiae, as a Generally Recognized as Safe (GRAS) fungus, has become one of the most widely used chassis cells for industrial applications and basic research.
Zhen Yao, Qinhong Wang, Zongjie Dai
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Directed evolution of improved zinc finger methyltransferases. [PDF]
PLoS ONE, 2014 The ability to target DNA methylation toward a single, user-designated CpG site in vivo may have wide applicability for basic biological and biomedical research.
Brian Chaikind, Marc Ostermeier
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