Results 21 to 30 of about 246,136 (236)
CRISPR enables directed evolution in plants
Genome Biology, 2019 A proof-of-concept study has demonstrated the application of CRISPR-Cas9 for directed evolution in rice, engineering crops for desired traits.
Yingxiao Zhang, Yiping Qi
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Cis Elements: Added Boost to the Directed Evolution of Plant Genes
Journal of Pure and Applied Microbiology, 2022 To increase the expression of a native/foreign plant/bacterial gene, the complete network of cis-elements must be excavated to increase its biosynthetic yield, especially under industrial stress conditions.
Harisomayajula Valli +6 more
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CADEE: Computer-Aided Directed Evolution of Enzymes
IUCrJ, 2017 The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a ...
Beat Anton Amrein +5 more
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Directed evolution in mammalian cells [PDF]
Nature Methods, 2021 Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environment severely constrains the scope and functionality of the targets that can be evolved.
Samuel J. Hendel, Matthew D. Shoulders
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Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution
Synthetic and Systems Biotechnology, 2021 Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a
Shangshang Sun, Chun You
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Laboratory-Directed Protein Evolution [PDF]
Microbiology and Molecular Biology Reviews, 2005 SUMMARYSystematic approaches to directed evolution of proteins have been documented since the 1970s. The ability to recruit new protein functions arises from the considerable substrate ambiguity of many proteins. The substrate ambiguity of a protein can be interpreted as the evolutionary potential that allows a protein to acquire new specificities ...
Ling, Yuan +3 more
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F1000Research, 2015 In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma +11 more
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PAIDDE: A Permutation-Archive Information Directed Differential Evolution Algorithm
IEEE Access, 2022 Evolutionary algorithms have shown great successes in various real-world applications ranging in molecule to astronomy. As a mainstream evolutionary algorithm, differential evolution (DE) possesses the characteristics of simple algorithmic structure ...
Xiaosi Li +5 more
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Directing evolution in cells [PDF]
Nature Methods, 2020 A CRISPR–Cas9-based approach generates libraries of mutant proteins targeted to specific subcellular compartments for in situ directed evolution.
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Directed Evolution and Biocatalysis [PDF]
Angewandte Chemie International Edition, 2001 This review describes the current state of biocatalysis in the chemical industry. Although we recognize the advantages of chemical approaches, we suggest that the use of biological catalysis is about to expand dramatically because of the recent developments in the artificial evolution of genes that code for enzymes. For the first time it is possible to
Keith A., Powell +6 more
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