Results 21 to 30 of about 1,372,167 (289)
Recent Advances in Directed Yeast Genome Evolution
Journal of Fungi, 2022 Saccharomyces cerevisiae, as a Generally Recognized as Safe (GRAS) fungus, has become one of the most widely used chassis cells for industrial applications and basic research.
Zhen Yao, Qinhong Wang, Zongjie Dai
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Directed evolution of improved zinc finger methyltransferases. [PDF]
PLoS ONE, 2014 The ability to target DNA methylation toward a single, user-designated CpG site in vivo may have wide applicability for basic biological and biomedical research.
Brian Chaikind, Marc Ostermeier
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Directed enzyme evolution: climbing fitness peaks one amino acid at a time [PDF]
, 2009 Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse.
Arnold, Frances H., Tracewell, Cara A.
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CRISPR enables directed evolution in plants
Genome Biology, 2019 A proof-of-concept study has demonstrated the application of CRISPR-Cas9 for directed evolution in rice, engineering crops for desired traits.
Yingxiao Zhang, Yiping Qi
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Cis Elements: Added Boost to the Directed Evolution of Plant Genes
Journal of Pure and Applied Microbiology, 2022 To increase the expression of a native/foreign plant/bacterial gene, the complete network of cis-elements must be excavated to increase its biosynthetic yield, especially under industrial stress conditions.
Harisomayajula Valli +6 more
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Evolution of ethnocentrism on undirected and directed Barabási-Albert networks [PDF]
, 2009 Using Monte Carlo simulations, we study the evolution of contigent cooperation and ethnocentrism in the one-move game. Interactions and reproduction among computational agents are simulated on undirected and directed Barabási-\ud Albert (BA) networks. We
Albert +50 more
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CADEE: Computer-Aided Directed Evolution of Enzymes
IUCrJ, 2017 The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a ...
Beat Anton Amrein +5 more
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Directed evolution in mammalian cells [PDF]
Nature Methods, 2021 Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environment severely constrains the scope and functionality of the targets that can be evolved.
Samuel J. Hendel, Matthew D. Shoulders
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Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution
Synthetic and Systems Biotechnology, 2021 Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a
Shangshang Sun, Chun You
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In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6 [PDF]
, 2009 Enzymes of the AlkB and CYP153 families catalyze the first step in the catabolism of medium-chain-length alkanes, selective oxidation of the alkane to the 1-alkanol, and enable their host organisms to utilize alkanes as carbon sources.
Arnold, Frances H. +3 more
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