Results 1 to 10 of about 495,896 (276)

Combinatorial Avidity Selection of Mosaic Landscape Phages Targeted at Breast Cancer Cells—An Alternative Mechanism of Directed Molecular Evolution [PDF]

Viruses, 2019
Low performance of actively targeted nanomedicines required revision of the traditional drug targeting paradigm and stimulated the development of novel phage-programmed, self-navigating drug delivery vehicles.
Valery A. Petrenko, James W. Gillespie, Hai Xu, Tiffany O’Dell, Laura M. De Plano   +4 more
doaj   +2 more sources

A directed molecular evolution approach to improved immunogenicity of the HIV-1 envelope glycoprotein. [PDF]

PLoS ONE, 2011
A prophylactic vaccine is needed to slow the spread of HIV-1 infection. Optimization of the wild-type envelope glycoproteins to create immunogens that can elicit effective neutralizing antibodies is a high priority.
Sean X Du, Li Xu, Wenge Zhang, Susan Tang, Rebecca I Boenig, Helen Chen, Ellaine B Mariano, Michael B Zwick, Paul W H I Parren, Dennis R Burton, Terri Wrin, Christos J Petropoulos, John A Ballantyne, Michael Chambers, Robert G Whalen   +14 more
doaj   +2 more sources

DNA Damage Protection for Enhanced Bacterial Survival Under Simulated Low Earth Orbit Environmental Conditions in Escherichia coli

Frontiers in Microbiology, 2021
Some organisms have shown the ability to naturally survive in extreme environments, even outer space. Some of these have natural mechanisms to resist severe DNA damage from conditions such as ionizing and non-ionizing radiation, extreme temperatures, and
Jaume Puig, Jaume Puig, Nastassia Knödlseder, Nastassia Knödlseder, Jaume Quera, Jaume Quera, Jaume Quera, Manuel Algara, Manuel Algara, Manuel Algara, Marc Güell, Marc Güell   +11 more
doaj   +1 more source

Optimal Evolutionary Control for Artificial Selection on Molecular Phenotypes

Physical Review X, 2021
Controlling an evolving population is an important task in modern molecular genetics, including directed evolution for improving the activity of molecules and enzymes, in breeding experiments in animals and in plants, and in devising public health ...
Armita Nourmohammad, Ceyhun Eksin
doaj   +1 more source

Improved catalytic performance and molecular insight for lipoxygenase from Enterovibrio norvegicus via directed evolution

Frontiers in Bioengineering and Biotechnology, 2023
Lipoxygenase (LOX) holds significant promise for food and pharmaceutical industries. However, albeit its application has been hampered by low catalytic activity and suboptimal thermostability.
Bingjie Zhang, Huibing Chi, Juan Shen, Yang Tao, Zhaoxin Lu, Fengxia Lu, Ping Zhu   +6 more
doaj   +1 more source

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

eLife, 2022
Alteration of antibiotic binding sites through modification of ribosomal RNA (rRNA) is a common form of resistance to ribosome-targeting antibiotics. The rRNA-modifying enzyme Cfr methylates an adenosine nucleotide within the peptidyl transferase center,
Kaitlyn Tsai, Vanja Stojković, Lianet Noda-Garcia, Iris D Young, Alexander G Myasnikov, Jordan Kleinman, Ali Palla, Stephen N Floor, Adam Frost, James S Fraser, Dan S Tawfik, Danica Galonić Fujimori   +11 more
doaj   +1 more source

Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening

Bioresources and Bioprocessing, 2022
As an important monomer for bio-based nylons PA5X, cadaverine is mainly produced by enzymatic decarboxylation of L-lysine. A key issue with this process is the instability of L-lysine decarboxylase (CadA) during the reaction due to the dissociation of ...
Yang Xi, Lidan Ye, Hongwei Yu
doaj   +1 more source

Prediction of designer-recombinases for DNA editing with generative deep learning

Nature Communications, 2022
Design of recombinases with new target sites is usually achieved through cycles of directed molecular evolution. Here the authors report Recombinase Generator, RecGen, an algorithm for generation of designer-recombinases; they perform experimental ...
Lukas Theo Schmitt, Maciej Paszkowski-Rogacz, Florian Jug, Frank Buchholz   +3 more
doaj   +1 more source

Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle [version 1; referees: 2 approved]

F1000Research, 2015
In prokaryotes, horizontal gene transfer (HGT) has been regarded as an important evolutionary drive to acquire and retain beneficial genes for their survival in diverse ecologies.
Punita Sharma, Tanwee Das De, Swati Sharma, Ashwani Kumar Mishra, Tina Thomas, Sonia Verma, Vandana Kumari, Suman Lata, Namita Singh, Neena Valecha, Kailash Chand Pandey, Rajnikant Dixit   +11 more
doaj   +1 more source

Known Evolutionary Paths Are Accessible to Engineered ß-Lactamases Having Altered Protein Motions at the Timescale of Catalytic Turnover

Frontiers in Molecular Biosciences, 2020
The evolution of new protein functions is dependent upon inherent biophysical features of proteins. Whereas, it has been shown that changes in protein dynamics can occur in the course of directed molecular evolution trajectories and contribute to new ...
Lorea Alejaldre, Lorea Alejaldre, Lorea Alejaldre, Claudèle Lemay-St-Denis, Claudèle Lemay-St-Denis, Claudèle Lemay-St-Denis, Carles Perez Lopez, Ferran Sancho Jodar, Victor Guallar, Victor Guallar, Joelle N. Pelletier, Joelle N. Pelletier, Joelle N. Pelletier, Joelle N. Pelletier   +13 more
doaj   +1 more source